PURPLE GUINEA GRASS: PRETREATMENT AND ETHANOL FERMENTATION
Treatment with dilute sulfuric acid (H2SO4) or calcium hydroxide (Ca(OH)2) at 121C and 103.4 kPa was used to improve the efficiency of the cellulose digestion of purple guinea grass. Cellulase hydrolysis of the dilute H2SO4-pretreated purple guinea grass under optimized conditions (6% (w/v) in 3% (...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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North Carolina State University
2012-02-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_07_2_1891_Ratsamee_ALSKT_Purple_Guinea_Grass_Pretreat_Ethanol/1468 |
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| author | Suvapatr Ratsamee, Ancharida Akaracharanya, Natchanun Leepipatpiboon , Teerapatr Srinorakutara, Vichien Kitpreechavanich, Vasana Tolieng |
| author_facet | Suvapatr Ratsamee, Ancharida Akaracharanya, Natchanun Leepipatpiboon , Teerapatr Srinorakutara, Vichien Kitpreechavanich, Vasana Tolieng |
| author_sort | Suvapatr Ratsamee, |
| collection | DOAJ |
| description | Treatment with dilute sulfuric acid (H2SO4) or calcium hydroxide (Ca(OH)2) at 121C and 103.4 kPa was used to improve the efficiency of the cellulose digestion of purple guinea grass. Cellulase hydrolysis of the dilute H2SO4-pretreated purple guinea grass under optimized conditions (6% (w/v) in 3% (w/v) H2SO4 for 30 min) yielded a slightly higher level of reducing sugars than that from the Ca(OH)2 pretreatment under optimized conditions (6% (w/v) in 4% (w/v) Ca(OH)2 for 5 min). However, the level of glucose released from the Ca(OH)2-pretreated purple guinea grass was slightly higher than that from the dilute H2SO4 pretreatment. Ethanol fermentation, via the separate hydrolysis and fermentation (SHF) process using Saccharomyces cerevisiae, of the Ca(OH)2-pretreated purple guinea grass and then hydrolyzed with commercial cellulase (9 PFU/g, dry wt.) for 6 h yielded ethanol at 0.44 g/g glucose (0.21 g/g cellulose) within 48 h, while that from the simultaneous saccharification and fermentation process yielded 14.3% less ethanol at 0.18 g/g cellulose within 96 h (including the 6 h saccharification time). The ethanol yield from the SHF process increased 1.14-fold to 0.497 g/g glucose (0.24 g/g cellulose) when the fermentation was performed in a 5 L fermentor. |
| first_indexed | 2024-04-12T12:56:24Z |
| format | Article |
| id | doaj.art-dfe5436d0567423494cee5ac017e210e |
| institution | Directory Open Access Journal |
| issn | 1930-2126 |
| language | English |
| last_indexed | 2024-04-12T12:56:24Z |
| publishDate | 2012-02-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-dfe5436d0567423494cee5ac017e210e2022-12-22T03:32:19ZengNorth Carolina State UniversityBioResources1930-21262012-02-017218911906PURPLE GUINEA GRASS: PRETREATMENT AND ETHANOL FERMENTATIONSuvapatr Ratsamee,Ancharida Akaracharanya,Natchanun Leepipatpiboon, Teerapatr Srinorakutara,Vichien Kitpreechavanich,Vasana ToliengTreatment with dilute sulfuric acid (H2SO4) or calcium hydroxide (Ca(OH)2) at 121C and 103.4 kPa was used to improve the efficiency of the cellulose digestion of purple guinea grass. Cellulase hydrolysis of the dilute H2SO4-pretreated purple guinea grass under optimized conditions (6% (w/v) in 3% (w/v) H2SO4 for 30 min) yielded a slightly higher level of reducing sugars than that from the Ca(OH)2 pretreatment under optimized conditions (6% (w/v) in 4% (w/v) Ca(OH)2 for 5 min). However, the level of glucose released from the Ca(OH)2-pretreated purple guinea grass was slightly higher than that from the dilute H2SO4 pretreatment. Ethanol fermentation, via the separate hydrolysis and fermentation (SHF) process using Saccharomyces cerevisiae, of the Ca(OH)2-pretreated purple guinea grass and then hydrolyzed with commercial cellulase (9 PFU/g, dry wt.) for 6 h yielded ethanol at 0.44 g/g glucose (0.21 g/g cellulose) within 48 h, while that from the simultaneous saccharification and fermentation process yielded 14.3% less ethanol at 0.18 g/g cellulose within 96 h (including the 6 h saccharification time). The ethanol yield from the SHF process increased 1.14-fold to 0.497 g/g glucose (0.24 g/g cellulose) when the fermentation was performed in a 5 L fermentor.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_07_2_1891_Ratsamee_ALSKT_Purple_Guinea_Grass_Pretreat_Ethanol/1468Purple guinea grassEthanolPretreatment |
| spellingShingle | Suvapatr Ratsamee, Ancharida Akaracharanya, Natchanun Leepipatpiboon , Teerapatr Srinorakutara, Vichien Kitpreechavanich, Vasana Tolieng PURPLE GUINEA GRASS: PRETREATMENT AND ETHANOL FERMENTATION BioResources Purple guinea grass Ethanol Pretreatment |
| title | PURPLE GUINEA GRASS: PRETREATMENT AND ETHANOL FERMENTATION |
| title_full | PURPLE GUINEA GRASS: PRETREATMENT AND ETHANOL FERMENTATION |
| title_fullStr | PURPLE GUINEA GRASS: PRETREATMENT AND ETHANOL FERMENTATION |
| title_full_unstemmed | PURPLE GUINEA GRASS: PRETREATMENT AND ETHANOL FERMENTATION |
| title_short | PURPLE GUINEA GRASS: PRETREATMENT AND ETHANOL FERMENTATION |
| title_sort | purple guinea grass pretreatment and ethanol fermentation |
| topic | Purple guinea grass Ethanol Pretreatment |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_07_2_1891_Ratsamee_ALSKT_Purple_Guinea_Grass_Pretreat_Ethanol/1468 |
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