Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum Dots
<p>Abstract</p> <p>Nanoparticles were recently reported to be able to improve both efficiency and specificity in polymerase chain reaction (PCR). Here, CdTe QDs were introduced into multi-PCR systems. It was found that an appropriate concentration of CdTe QDs could enhance the perf...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2011-01-01
|
| Series: | Nanoscale Research Letters |
| Subjects: | |
| Online Access: | http://www.nanoscalereslett.com/content/6/1/51 |
| _version_ | 1797719254875766784 |
|---|---|
| author | Liang Gaofeng Ma Chao Zhu Yanliang Li Shuchun Shao Youhua Wang Yong Xiao Zhongdang |
| author_facet | Liang Gaofeng Ma Chao Zhu Yanliang Li Shuchun Shao Youhua Wang Yong Xiao Zhongdang |
| author_sort | Liang Gaofeng |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Nanoparticles were recently reported to be able to improve both efficiency and specificity in polymerase chain reaction (PCR). Here, CdTe QDs were introduced into multi-PCR systems. It was found that an appropriate concentration of CdTe QDs could enhance the performance of multi-PCR by reducing the formation of nonspecific products in the complex system, but an excessive amount of CdTe QDs could suppress the PCR. The effects of QDs on PCR can be reversed by increasing the polymerase concentration or by adding bovine serum albumin (BSA). The mechanisms underlying these effects were also discussed. The results indicated that CdTe QDs could be used to optimize the amplification products of the PCR, especially in the multi-PCR system with different primers annealing temperatures, which is of great significance for molecular diagnosis.</p> |
| first_indexed | 2024-03-12T09:02:11Z |
| format | Article |
| id | doaj.art-dfe6a60fda7c47369c234993080b8233 |
| institution | Directory Open Access Journal |
| issn | 1931-7573 1556-276X |
| language | English |
| last_indexed | 2024-03-12T09:02:11Z |
| publishDate | 2011-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-dfe6a60fda7c47369c234993080b82332023-09-02T15:37:20ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2011-01-016151Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum DotsLiang GaofengMa ChaoZhu YanliangLi ShuchunShao YouhuaWang YongXiao Zhongdang<p>Abstract</p> <p>Nanoparticles were recently reported to be able to improve both efficiency and specificity in polymerase chain reaction (PCR). Here, CdTe QDs were introduced into multi-PCR systems. It was found that an appropriate concentration of CdTe QDs could enhance the performance of multi-PCR by reducing the formation of nonspecific products in the complex system, but an excessive amount of CdTe QDs could suppress the PCR. The effects of QDs on PCR can be reversed by increasing the polymerase concentration or by adding bovine serum albumin (BSA). The mechanisms underlying these effects were also discussed. The results indicated that CdTe QDs could be used to optimize the amplification products of the PCR, especially in the multi-PCR system with different primers annealing temperatures, which is of great significance for molecular diagnosis.</p>http://www.nanoscalereslett.com/content/6/1/51PCRQuantum dotsSpecificity |
| spellingShingle | Liang Gaofeng Ma Chao Zhu Yanliang Li Shuchun Shao Youhua Wang Yong Xiao Zhongdang Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum Dots Nanoscale Research Letters PCR Quantum dots Specificity |
| title | Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum Dots |
| title_full | Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum Dots |
| title_fullStr | Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum Dots |
| title_full_unstemmed | Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum Dots |
| title_short | Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum Dots |
| title_sort | enhanced specificity of multiplex polymerase chain reaction via cdte quantum dots |
| topic | PCR Quantum dots Specificity |
| url | http://www.nanoscalereslett.com/content/6/1/51 |
| work_keys_str_mv | AT lianggaofeng enhancedspecificityofmultiplexpolymerasechainreactionviacdtequantumdots AT machao enhancedspecificityofmultiplexpolymerasechainreactionviacdtequantumdots AT zhuyanliang enhancedspecificityofmultiplexpolymerasechainreactionviacdtequantumdots AT lishuchun enhancedspecificityofmultiplexpolymerasechainreactionviacdtequantumdots AT shaoyouhua enhancedspecificityofmultiplexpolymerasechainreactionviacdtequantumdots AT wangyong enhancedspecificityofmultiplexpolymerasechainreactionviacdtequantumdots AT xiaozhongdang enhancedspecificityofmultiplexpolymerasechainreactionviacdtequantumdots |