Phytochemicals Profiling, Antimicrobial Activity and Mechanism of Action of Essential Oil Extracted from Ginger (<i>Zingiber officinale</i> Roscoe cv. Bentong) against <i>Burkholderia glumae</i> Causative Agent of Bacterial Panicle Blight Disease of Rice
Essential oils protect plants, and due to their natural origin, there is much interest in using them as antimicrobial agents. The purpose of this study was to determine the phytochemical constituents of ginger essential oil (GEO), antimicrobial activity, and mode of action against <i>Burkholde...
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MDPI AG
2022-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/11/1466 |
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| author | Mahesh Tiran Gunasena Amara Rafi Syazwan Afif Mohd Zobir Mohd Zobir Hussein Asgar Ali Abdulaziz Bashir Kutawa Mohd Aswad Abdul Wahab Mohd Roslan Sulaiman Fariz Adzmi Khairulmazmi Ahmad |
| author_facet | Mahesh Tiran Gunasena Amara Rafi Syazwan Afif Mohd Zobir Mohd Zobir Hussein Asgar Ali Abdulaziz Bashir Kutawa Mohd Aswad Abdul Wahab Mohd Roslan Sulaiman Fariz Adzmi Khairulmazmi Ahmad |
| author_sort | Mahesh Tiran Gunasena |
| collection | DOAJ |
| description | Essential oils protect plants, and due to their natural origin, there is much interest in using them as antimicrobial agents. The purpose of this study was to determine the phytochemical constituents of ginger essential oil (GEO), antimicrobial activity, and mode of action against <i>Burkholderia glumae</i> (<i>Bg</i>). In addition, the volatile active compounds (AIs) were studied using GC-MS, FTIR, and Raman spectroscopy. A total of 45 phytochemical components were detected and the most prevalent bioactive compounds were Geranial, 1,8-Cineole, Neral, Camphene, α-Zingiberene, and α-Farnesene. Furthermore, it was found that the most dominant terpenes in GEO were monoterpenes. The diameter zone of inhibition values varied from 7.1 to 15 mm depending on the concentration tested. In addition, the MIC and MBC values were 112.5 µL/mL. Faster killing time and lower membrane potential were observed in 1xMIC treatment compared to 0.5xMIC treatment, whereas the control had the maximum values. From observations of various images, it was concluded that the mode of action of GEO affected the cytoplasmic membrane, causing it to lose its integrity and increase its permeability. Therefore, the antibacterial study and mechanism of action revealed that GEO is very effective in suppressing the growth of <i>B. glumae</i>. |
| first_indexed | 2024-03-10T00:59:06Z |
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| id | doaj.art-dfeab125cae2430d8ec493649161ce0f |
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| language | English |
| last_indexed | 2024-03-10T00:59:06Z |
| publishDate | 2022-05-01 |
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| series | Plants |
| spelling | doaj.art-dfeab125cae2430d8ec493649161ce0f2023-11-23T14:39:07ZengMDPI AGPlants2223-77472022-05-011111146610.3390/plants11111466Phytochemicals Profiling, Antimicrobial Activity and Mechanism of Action of Essential Oil Extracted from Ginger (<i>Zingiber officinale</i> Roscoe cv. Bentong) against <i>Burkholderia glumae</i> Causative Agent of Bacterial Panicle Blight Disease of RiceMahesh Tiran Gunasena0Amara Rafi1Syazwan Afif Mohd Zobir2Mohd Zobir Hussein3Asgar Ali4Abdulaziz Bashir Kutawa5Mohd Aswad Abdul Wahab6Mohd Roslan Sulaiman7Fariz Adzmi8Khairulmazmi Ahmad9Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, MalaysiaDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, MalaysiaDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, MalaysiaInstitute of Nanoscience and Nanotechnology, Universiti Putra Malaysia, Serdang 43400, MalaysiaCentre of Excellence for Postharvest Biotechnology (CEPB), School of Biosciences, University of Nottingham Malaysia, Semenyih 43500, MalaysiaDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, MalaysiaDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, MalaysiaDepartment of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, MalaysiaInstitute of Plantation Studies (IKP), Universiti Putra Malaysia, Serdang 43400, MalaysiaDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, MalaysiaEssential oils protect plants, and due to their natural origin, there is much interest in using them as antimicrobial agents. The purpose of this study was to determine the phytochemical constituents of ginger essential oil (GEO), antimicrobial activity, and mode of action against <i>Burkholderia glumae</i> (<i>Bg</i>). In addition, the volatile active compounds (AIs) were studied using GC-MS, FTIR, and Raman spectroscopy. A total of 45 phytochemical components were detected and the most prevalent bioactive compounds were Geranial, 1,8-Cineole, Neral, Camphene, α-Zingiberene, and α-Farnesene. Furthermore, it was found that the most dominant terpenes in GEO were monoterpenes. The diameter zone of inhibition values varied from 7.1 to 15 mm depending on the concentration tested. In addition, the MIC and MBC values were 112.5 µL/mL. Faster killing time and lower membrane potential were observed in 1xMIC treatment compared to 0.5xMIC treatment, whereas the control had the maximum values. From observations of various images, it was concluded that the mode of action of GEO affected the cytoplasmic membrane, causing it to lose its integrity and increase its permeability. Therefore, the antibacterial study and mechanism of action revealed that GEO is very effective in suppressing the growth of <i>B. glumae</i>.https://www.mdpi.com/2223-7747/11/11/1466antimicrobial activitybioactive compounds<i>Burkholderia glumae</i>gas chromatographyginger essential oil |
| spellingShingle | Mahesh Tiran Gunasena Amara Rafi Syazwan Afif Mohd Zobir Mohd Zobir Hussein Asgar Ali Abdulaziz Bashir Kutawa Mohd Aswad Abdul Wahab Mohd Roslan Sulaiman Fariz Adzmi Khairulmazmi Ahmad Phytochemicals Profiling, Antimicrobial Activity and Mechanism of Action of Essential Oil Extracted from Ginger (<i>Zingiber officinale</i> Roscoe cv. Bentong) against <i>Burkholderia glumae</i> Causative Agent of Bacterial Panicle Blight Disease of Rice Plants antimicrobial activity bioactive compounds <i>Burkholderia glumae</i> gas chromatography ginger essential oil |
| title | Phytochemicals Profiling, Antimicrobial Activity and Mechanism of Action of Essential Oil Extracted from Ginger (<i>Zingiber officinale</i> Roscoe cv. Bentong) against <i>Burkholderia glumae</i> Causative Agent of Bacterial Panicle Blight Disease of Rice |
| title_full | Phytochemicals Profiling, Antimicrobial Activity and Mechanism of Action of Essential Oil Extracted from Ginger (<i>Zingiber officinale</i> Roscoe cv. Bentong) against <i>Burkholderia glumae</i> Causative Agent of Bacterial Panicle Blight Disease of Rice |
| title_fullStr | Phytochemicals Profiling, Antimicrobial Activity and Mechanism of Action of Essential Oil Extracted from Ginger (<i>Zingiber officinale</i> Roscoe cv. Bentong) against <i>Burkholderia glumae</i> Causative Agent of Bacterial Panicle Blight Disease of Rice |
| title_full_unstemmed | Phytochemicals Profiling, Antimicrobial Activity and Mechanism of Action of Essential Oil Extracted from Ginger (<i>Zingiber officinale</i> Roscoe cv. Bentong) against <i>Burkholderia glumae</i> Causative Agent of Bacterial Panicle Blight Disease of Rice |
| title_short | Phytochemicals Profiling, Antimicrobial Activity and Mechanism of Action of Essential Oil Extracted from Ginger (<i>Zingiber officinale</i> Roscoe cv. Bentong) against <i>Burkholderia glumae</i> Causative Agent of Bacterial Panicle Blight Disease of Rice |
| title_sort | phytochemicals profiling antimicrobial activity and mechanism of action of essential oil extracted from ginger i zingiber officinale i roscoe cv bentong against i burkholderia glumae i causative agent of bacterial panicle blight disease of rice |
| topic | antimicrobial activity bioactive compounds <i>Burkholderia glumae</i> gas chromatography ginger essential oil |
| url | https://www.mdpi.com/2223-7747/11/11/1466 |
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