PROTON-PUMPING AND NITROGEN METABOLISM IN RICE UNDER HUMID TROPIC CONDITIONS
In rice plants, it is important to recognize N metabolism efficiency parameters in order to design strategies for improvements, increasing grain protein and enhancing people nutrition quality. Two rice varieties grown under humid tropic conditions were evaluated: Piaui, a landrace adapted to low N a...
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| Format: | Article |
| Language: | English |
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Universidad Autónoma de Yucatán
2012-12-01
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| Series: | Tropical and Subtropical Agroecosystems |
| Subjects: | |
| Online Access: | http://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/1211 |
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| author | Rodrigo Grazinoli Garrido Fabiola de S.R.G. Garrido Sonia Regina de Souza Manlio Silvestre Fernandes |
| author_facet | Rodrigo Grazinoli Garrido Fabiola de S.R.G. Garrido Sonia Regina de Souza Manlio Silvestre Fernandes |
| author_sort | Rodrigo Grazinoli Garrido |
| collection | DOAJ |
| description | In rice plants, it is important to recognize N metabolism efficiency parameters in order to design strategies for improvements, increasing grain protein and enhancing people nutrition quality. Two rice varieties grown under humid tropic conditions were evaluated: Piaui, a landrace adapted to low N availability, and reduced light supply, and IAC-47, an improved variety. The assay was carried out in controlled conditions of 24oC and 12/12 hour light/dark periods (200 µE.m-2.s-1) using a growth chamber, simulating humid tropic environment. Rice plants were grown under 0.1 and 1.0 mM NH4+-N, in a Hoagland & Arnon nutrient solution, pH 5.5, up to 26 days after germination. The activity of PM H+-ATPases in the plasma membrane, vacuole VH+-ATPases, H+-PPases as well as GS, GOGAT, and GDH were determined. PM H+-ATPase presented higher activity in IAC-47 roots. On the other hand, Piaui roots showed an enhanced microsomal protein content and VH+-ATPases activity, which apparently allows this variety to absorb N as well as the improved one. Increases in the cytosolic ammonium would result in high GDH deamination in Piaui variety at 1.0 mM NH4+-N. Meanwhile, GS increases were observed in IAC-47 shoots, together with GOGAT increases at same treatment. |
| first_indexed | 2024-04-12T05:13:12Z |
| format | Article |
| id | doaj.art-dff09dd7f60a4ce1803323e749719760 |
| institution | Directory Open Access Journal |
| issn | 1870-0462 |
| language | English |
| last_indexed | 2024-04-12T05:13:12Z |
| publishDate | 2012-12-01 |
| publisher | Universidad Autónoma de Yucatán |
| record_format | Article |
| series | Tropical and Subtropical Agroecosystems |
| spelling | doaj.art-dff09dd7f60a4ce1803323e7497197602022-12-22T03:46:42ZengUniversidad Autónoma de YucatánTropical and Subtropical Agroecosystems1870-04622012-12-01153521PROTON-PUMPING AND NITROGEN METABOLISM IN RICE UNDER HUMID TROPIC CONDITIONSRodrigo Grazinoli Garrido0Fabiola de S.R.G. Garrido1Sonia Regina de Souza2Manlio Silvestre Fernandes3Institute of Forensic Genetic Research - IPPGF/DGPTC/PCERJEnvironmental and Administrative Science Department, ITR, UFRRJChemistry Department, ICE, UFRRJSoils Department, IA, UFRRJIn rice plants, it is important to recognize N metabolism efficiency parameters in order to design strategies for improvements, increasing grain protein and enhancing people nutrition quality. Two rice varieties grown under humid tropic conditions were evaluated: Piaui, a landrace adapted to low N availability, and reduced light supply, and IAC-47, an improved variety. The assay was carried out in controlled conditions of 24oC and 12/12 hour light/dark periods (200 µE.m-2.s-1) using a growth chamber, simulating humid tropic environment. Rice plants were grown under 0.1 and 1.0 mM NH4+-N, in a Hoagland & Arnon nutrient solution, pH 5.5, up to 26 days after germination. The activity of PM H+-ATPases in the plasma membrane, vacuole VH+-ATPases, H+-PPases as well as GS, GOGAT, and GDH were determined. PM H+-ATPase presented higher activity in IAC-47 roots. On the other hand, Piaui roots showed an enhanced microsomal protein content and VH+-ATPases activity, which apparently allows this variety to absorb N as well as the improved one. Increases in the cytosolic ammonium would result in high GDH deamination in Piaui variety at 1.0 mM NH4+-N. Meanwhile, GS increases were observed in IAC-47 shoots, together with GOGAT increases at same treatment.http://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/1211Oryza sativaAmmoniumV-ATPaseP-ATPaseV-PPaseGSGOGATGDH |
| spellingShingle | Rodrigo Grazinoli Garrido Fabiola de S.R.G. Garrido Sonia Regina de Souza Manlio Silvestre Fernandes PROTON-PUMPING AND NITROGEN METABOLISM IN RICE UNDER HUMID TROPIC CONDITIONS Tropical and Subtropical Agroecosystems Oryza sativa Ammonium V-ATPase P-ATPase V-PPase GS GOGAT GDH |
| title | PROTON-PUMPING AND NITROGEN METABOLISM IN RICE UNDER HUMID TROPIC CONDITIONS |
| title_full | PROTON-PUMPING AND NITROGEN METABOLISM IN RICE UNDER HUMID TROPIC CONDITIONS |
| title_fullStr | PROTON-PUMPING AND NITROGEN METABOLISM IN RICE UNDER HUMID TROPIC CONDITIONS |
| title_full_unstemmed | PROTON-PUMPING AND NITROGEN METABOLISM IN RICE UNDER HUMID TROPIC CONDITIONS |
| title_short | PROTON-PUMPING AND NITROGEN METABOLISM IN RICE UNDER HUMID TROPIC CONDITIONS |
| title_sort | proton pumping and nitrogen metabolism in rice under humid tropic conditions |
| topic | Oryza sativa Ammonium V-ATPase P-ATPase V-PPase GS GOGAT GDH |
| url | http://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/1211 |
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