MicroRNAs and their regulatory networks in Chinese Gushi chicken abdominal adipose tissue during postnatal late development
Abstract Background Abdominal fat is the major adipose tissue in chickens. The growth status of abdominal fat during postnatal late development ultimately affects meat yield and quality in chickens. MicroRNAs (miRNAs) are endogenous small noncoding RNAs that regulate gene expression at the post-tran...
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| Format: | Article |
| Language: | English |
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BMC
2019-10-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-019-6094-2 |
| _version_ | 1828481934670430208 |
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| author | Yi Chen Yinli Zhao Wenjiao Jin Yuanfang Li Yanhua Zhang Xuejie Ma Guirong Sun Ruili Han Yadong Tian Hong Li Xiangtao Kang Guoxi Li |
| author_facet | Yi Chen Yinli Zhao Wenjiao Jin Yuanfang Li Yanhua Zhang Xuejie Ma Guirong Sun Ruili Han Yadong Tian Hong Li Xiangtao Kang Guoxi Li |
| author_sort | Yi Chen |
| collection | DOAJ |
| description | Abstract Background Abdominal fat is the major adipose tissue in chickens. The growth status of abdominal fat during postnatal late development ultimately affects meat yield and quality in chickens. MicroRNAs (miRNAs) are endogenous small noncoding RNAs that regulate gene expression at the post-transcriptional level. Studies have shown that miRNAs play an important role in the biological processes involved in adipose tissue development. However, few studies have investigated miRNA expression profiles and their interaction networks associated with the postnatal late development of abdominal adipose tissue in chickens. Results We constructed four small RNA libraries from abdominal adipose tissue obtained from Chinese domestic Gushi chickens at 6, 14, 22, and 30 weeks. A total of 507 known miRNAs and 53 novel miRNAs were identified based on the four small RNA libraries. Fifty-one significant differentially expressed (SDE) miRNAs were identified from six combinations by comparative analysis, and the expression patterns of these SDE miRNAs were divided into six subclusters by cluster analysis. Gene ontology enrichment analysis showed that the SDE miRNAs were primarily involved in the regulation of fat cell differentiation, regulation of lipid metabolism, regulation of fatty acid metabolism, and unsaturated fatty acid metabolism in the lipid metabolism- or deposition-related biological process categories. In addition, we constructed differentially expressed miRNA–mRNA interaction networks related to abdominal adipose development. The results showed that miRNA families, such as mir-30, mir-34, mir-199, mir-8, and mir-146, may have key roles in lipid metabolism, adipocyte proliferation and differentiation, and cell junctions during abdominal adipose tissue development in chickens. Conclusions This study determined the dynamic miRNA transcriptome and characterized the miRNA–mRNA interaction networks in Gushi chicken abdominal adipose tissue for the first time. The results expanded the number of known miRNAs in abdominal adipose tissue and provide novel insights and a valuable resource to elucidate post-transcriptional regulation mechanisms during postnatal late development of abdominal adipose tissue in chicken. |
| first_indexed | 2024-12-11T08:04:41Z |
| format | Article |
| id | doaj.art-dff27b40f2024f44b7b9456dbe5218a9 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-11T08:04:41Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-dff27b40f2024f44b7b9456dbe5218a92022-12-22T01:15:01ZengBMCBMC Genomics1471-21642019-10-0120111810.1186/s12864-019-6094-2MicroRNAs and their regulatory networks in Chinese Gushi chicken abdominal adipose tissue during postnatal late developmentYi Chen0Yinli Zhao1Wenjiao Jin2Yuanfang Li3Yanhua Zhang4Xuejie Ma5Guirong Sun6Ruili Han7Yadong Tian8Hong Li9Xiangtao Kang10Guoxi Li11College of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Biological Engineering, Henan University of TechnologyCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityAbstract Background Abdominal fat is the major adipose tissue in chickens. The growth status of abdominal fat during postnatal late development ultimately affects meat yield and quality in chickens. MicroRNAs (miRNAs) are endogenous small noncoding RNAs that regulate gene expression at the post-transcriptional level. Studies have shown that miRNAs play an important role in the biological processes involved in adipose tissue development. However, few studies have investigated miRNA expression profiles and their interaction networks associated with the postnatal late development of abdominal adipose tissue in chickens. Results We constructed four small RNA libraries from abdominal adipose tissue obtained from Chinese domestic Gushi chickens at 6, 14, 22, and 30 weeks. A total of 507 known miRNAs and 53 novel miRNAs were identified based on the four small RNA libraries. Fifty-one significant differentially expressed (SDE) miRNAs were identified from six combinations by comparative analysis, and the expression patterns of these SDE miRNAs were divided into six subclusters by cluster analysis. Gene ontology enrichment analysis showed that the SDE miRNAs were primarily involved in the regulation of fat cell differentiation, regulation of lipid metabolism, regulation of fatty acid metabolism, and unsaturated fatty acid metabolism in the lipid metabolism- or deposition-related biological process categories. In addition, we constructed differentially expressed miRNA–mRNA interaction networks related to abdominal adipose development. The results showed that miRNA families, such as mir-30, mir-34, mir-199, mir-8, and mir-146, may have key roles in lipid metabolism, adipocyte proliferation and differentiation, and cell junctions during abdominal adipose tissue development in chickens. Conclusions This study determined the dynamic miRNA transcriptome and characterized the miRNA–mRNA interaction networks in Gushi chicken abdominal adipose tissue for the first time. The results expanded the number of known miRNAs in abdominal adipose tissue and provide novel insights and a valuable resource to elucidate post-transcriptional regulation mechanisms during postnatal late development of abdominal adipose tissue in chicken.http://link.springer.com/article/10.1186/s12864-019-6094-2Abdominal adipose tissueChickenInteraction networksMicroRNA |
| spellingShingle | Yi Chen Yinli Zhao Wenjiao Jin Yuanfang Li Yanhua Zhang Xuejie Ma Guirong Sun Ruili Han Yadong Tian Hong Li Xiangtao Kang Guoxi Li MicroRNAs and their regulatory networks in Chinese Gushi chicken abdominal adipose tissue during postnatal late development BMC Genomics Abdominal adipose tissue Chicken Interaction networks MicroRNA |
| title | MicroRNAs and their regulatory networks in Chinese Gushi chicken abdominal adipose tissue during postnatal late development |
| title_full | MicroRNAs and their regulatory networks in Chinese Gushi chicken abdominal adipose tissue during postnatal late development |
| title_fullStr | MicroRNAs and their regulatory networks in Chinese Gushi chicken abdominal adipose tissue during postnatal late development |
| title_full_unstemmed | MicroRNAs and their regulatory networks in Chinese Gushi chicken abdominal adipose tissue during postnatal late development |
| title_short | MicroRNAs and their regulatory networks in Chinese Gushi chicken abdominal adipose tissue during postnatal late development |
| title_sort | micrornas and their regulatory networks in chinese gushi chicken abdominal adipose tissue during postnatal late development |
| topic | Abdominal adipose tissue Chicken Interaction networks MicroRNA |
| url | http://link.springer.com/article/10.1186/s12864-019-6094-2 |
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