Saponin-Derived Silver Nanoparticles from <i>Phoenix dactylifera</i> (Ajwa Dates) Exhibit Broad-Spectrum Bioactivities Combating Bacterial Infections
The emergence of antibiotic resistance poses a serious threat to humankind, emphasizing the need for alternative antimicrobial agents. This study focuses on investigating the antibacterial, antibiofilm, and anti-quorum-sensing (anti-QS) activities of saponin-derived silver nanoparticles (AgNPs-S) ob...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/9/1415 |
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| author | Mohd Adnan Arif Jamal Siddiqui Syed Amir Ashraf Mohammad Saquib Ashraf Sarah Owdah Alomrani Mousa Alreshidi Bektas Tepe Manojkumar Sachidanandan Corina Danciu Mitesh Patel |
| author_facet | Mohd Adnan Arif Jamal Siddiqui Syed Amir Ashraf Mohammad Saquib Ashraf Sarah Owdah Alomrani Mousa Alreshidi Bektas Tepe Manojkumar Sachidanandan Corina Danciu Mitesh Patel |
| author_sort | Mohd Adnan |
| collection | DOAJ |
| description | The emergence of antibiotic resistance poses a serious threat to humankind, emphasizing the need for alternative antimicrobial agents. This study focuses on investigating the antibacterial, antibiofilm, and anti-quorum-sensing (anti-QS) activities of saponin-derived silver nanoparticles (AgNPs-S) obtained from Ajwa dates (<i>Phoenix dactylifera</i> L.). The design and synthesis of these novel nanoparticles were explored in the context of developing alternative strategies to combat bacterial infections. The Ajwa date saponin extract was used as a reducing and stabilizing agent to synthesize AgNPs-S, which was characterized using various analytical techniques, including UV–Vis spectroscopy, Fourier transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM). The biosynthesized AgNPs-S exhibited potent antibacterial activity against both Gram-positive and Gram-negative bacteria due to their capability to disrupt bacterial cell membranes and the leakage of nucleic acid and protein contents. The AgNPs-S effectively inhibited biofilm formation and quorum-sensing (QS) activity by interfering with QS signaling molecules, which play a pivotal role in bacterial virulence and pathogenicity. Furthermore, the AgNPs-S demonstrated significant antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and cytotoxicity against small lung cancer cells (A549 cells). Overall, the findings of the present study provide valuable insights into the potential use of these nanoparticles as alternative therapeutic agents for the design and development of novel antibiotics. Further investigations are warranted to elucidate the possible mechanism involved and safety concerns when it is used in vivo, paving the way for future therapeutic applications in combating bacterial infections and overcoming antibiotic resistance. |
| first_indexed | 2024-03-10T23:06:55Z |
| format | Article |
| id | doaj.art-dff34aaf4511420dbe7cff3f6ceed561 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T23:06:55Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-dff34aaf4511420dbe7cff3f6ceed5612023-11-19T09:17:27ZengMDPI AGAntibiotics2079-63822023-09-01129141510.3390/antibiotics12091415Saponin-Derived Silver Nanoparticles from <i>Phoenix dactylifera</i> (Ajwa Dates) Exhibit Broad-Spectrum Bioactivities Combating Bacterial InfectionsMohd Adnan0Arif Jamal Siddiqui1Syed Amir Ashraf2Mohammad Saquib Ashraf3Sarah Owdah Alomrani4Mousa Alreshidi5Bektas Tepe6Manojkumar Sachidanandan7Corina Danciu8Mitesh Patel9Department of Biology, College of Science, University of Ha’il, Ha’il 55473, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Ha’il 55473, Saudi ArabiaMedical and Diagnostic Research Centre, University of Ha’il, Ha’il 55473, Saudi ArabiaDepartment of Medical Laboratory Science, College of Applied Medical Sciences, Riyadh ELM University, Riyadh 12734, Saudi ArabiaDepartment of Biology, College of Science and Arts, Najran University, Najran 66252, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Ha’il 55473, Saudi ArabiaDepartment of Molecular Biology and Genetics, Faculty of Science and Literature, Kilis 7 Aralik University, TR-79000 Kilis, TurkeyMedical and Diagnostic Research Centre, University of Ha’il, Ha’il 55473, Saudi ArabiaDepartment of Pharmacognosy, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaResearch and Development Cell, Department of Biotechnology, Parul Institute of Applied Sciences, Parul University, Vadodara 391760, IndiaThe emergence of antibiotic resistance poses a serious threat to humankind, emphasizing the need for alternative antimicrobial agents. This study focuses on investigating the antibacterial, antibiofilm, and anti-quorum-sensing (anti-QS) activities of saponin-derived silver nanoparticles (AgNPs-S) obtained from Ajwa dates (<i>Phoenix dactylifera</i> L.). The design and synthesis of these novel nanoparticles were explored in the context of developing alternative strategies to combat bacterial infections. The Ajwa date saponin extract was used as a reducing and stabilizing agent to synthesize AgNPs-S, which was characterized using various analytical techniques, including UV–Vis spectroscopy, Fourier transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM). The biosynthesized AgNPs-S exhibited potent antibacterial activity against both Gram-positive and Gram-negative bacteria due to their capability to disrupt bacterial cell membranes and the leakage of nucleic acid and protein contents. The AgNPs-S effectively inhibited biofilm formation and quorum-sensing (QS) activity by interfering with QS signaling molecules, which play a pivotal role in bacterial virulence and pathogenicity. Furthermore, the AgNPs-S demonstrated significant antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and cytotoxicity against small lung cancer cells (A549 cells). Overall, the findings of the present study provide valuable insights into the potential use of these nanoparticles as alternative therapeutic agents for the design and development of novel antibiotics. Further investigations are warranted to elucidate the possible mechanism involved and safety concerns when it is used in vivo, paving the way for future therapeutic applications in combating bacterial infections and overcoming antibiotic resistance.https://www.mdpi.com/2079-6382/12/9/1415antibiofilmanti-quorum sensingcytotoxicityantibacterialantibiotic resistancesaponins |
| spellingShingle | Mohd Adnan Arif Jamal Siddiqui Syed Amir Ashraf Mohammad Saquib Ashraf Sarah Owdah Alomrani Mousa Alreshidi Bektas Tepe Manojkumar Sachidanandan Corina Danciu Mitesh Patel Saponin-Derived Silver Nanoparticles from <i>Phoenix dactylifera</i> (Ajwa Dates) Exhibit Broad-Spectrum Bioactivities Combating Bacterial Infections Antibiotics antibiofilm anti-quorum sensing cytotoxicity antibacterial antibiotic resistance saponins |
| title | Saponin-Derived Silver Nanoparticles from <i>Phoenix dactylifera</i> (Ajwa Dates) Exhibit Broad-Spectrum Bioactivities Combating Bacterial Infections |
| title_full | Saponin-Derived Silver Nanoparticles from <i>Phoenix dactylifera</i> (Ajwa Dates) Exhibit Broad-Spectrum Bioactivities Combating Bacterial Infections |
| title_fullStr | Saponin-Derived Silver Nanoparticles from <i>Phoenix dactylifera</i> (Ajwa Dates) Exhibit Broad-Spectrum Bioactivities Combating Bacterial Infections |
| title_full_unstemmed | Saponin-Derived Silver Nanoparticles from <i>Phoenix dactylifera</i> (Ajwa Dates) Exhibit Broad-Spectrum Bioactivities Combating Bacterial Infections |
| title_short | Saponin-Derived Silver Nanoparticles from <i>Phoenix dactylifera</i> (Ajwa Dates) Exhibit Broad-Spectrum Bioactivities Combating Bacterial Infections |
| title_sort | saponin derived silver nanoparticles from i phoenix dactylifera i ajwa dates exhibit broad spectrum bioactivities combating bacterial infections |
| topic | antibiofilm anti-quorum sensing cytotoxicity antibacterial antibiotic resistance saponins |
| url | https://www.mdpi.com/2079-6382/12/9/1415 |
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