α-Mangostin Mediated Pharmacological Modulation of Hepatic Carbohydrate Metabolism in Diabetes Induced Wistar Rat
Garcinia mangostana L. (Fruit) has been commonly used as folklore drug in the treatment of various types of diseases. The present experiment was designed to evaluate the potential effect of α-mangostin mediated pharmacological modulation of hepatic carbohydrate metabolism in streptozotocin (STZ) ind...
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SpringerOpen
2016-09-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2314853516300622 |
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| author | Vikas Kumar Prakash Chandra Bhatt Gaurav Kaithwas Mohd Rashid F.A. Al-abbasi Jalaluddin A.J. Khan Firoz Anwar Amita Verma |
| author_facet | Vikas Kumar Prakash Chandra Bhatt Gaurav Kaithwas Mohd Rashid F.A. Al-abbasi Jalaluddin A.J. Khan Firoz Anwar Amita Verma |
| author_sort | Vikas Kumar |
| collection | DOAJ |
| description | Garcinia mangostana L. (Fruit) has been commonly used as folklore drug in the treatment of various types of diseases. The present experiment was designed to evaluate the potential effect of α-mangostin mediated pharmacological modulation of hepatic carbohydrate metabolism in streptozotocin (STZ) induced diabetic rats. Oral glucose tolerance test (OGTT) was performed in normoglycemic rats. Single intraperitoneal injection of STZ (60 mg/kg, body weight) was used for induction the diabetes in Swiss albino (Wistar strain) rats. The rats were divided into different groups. Blood glucose level, body weight, insulin, glycated hemoglobin and hemoglobin levels were recorded at regular intervals. Biochemical parameters, liver enzymes, lipid profile, antioxidant parameters and inflammatory cytokine mediators were also scrutinized. Histopathology study of kidney, pancreas and liver were performed. The result of OGTT study depicted the better utilization of glucose in experimental rats. STZ induced diabetic rats treated with α-mangostin (25, 50 and 100 mg/kg, p.o.) and glibenclamide depicted the decline in the level of blood glucose; enhanced body weight and showed the better utilization of glucose by different organs. STZ induced diabetic rats treated with α-mangostin illustrated the increased level of plasma insulin, hemoglobin, hexokinase, HDL, total protein, SOD, CAT, GSH and declined level of glycated hemoglobin, fructose-1-6-biphosphatase, glucose-6-Phosphatase, TC, TG, LDL, VLDL, CRE, BUN, SGOT, SGPT, ALP and LPO at effective dose dependent manners. Histological study showed the inflamed blood vessels in diabetic kidney, which was less in α-mangostin treated rats; diabetic pancreatic showed the complete damage of β cells, islets, aciini and producing necrosis, but all damage was less obvious in α-mangostin treating group rats; diabetic liver showed the damage of hepatocytes as well as central vein but was less in treated groups. Considering the above results, α-mangostin shows potential to develop a medicine for diabetes, hyperlipidemia, renal and hepatic protection as combinational or mono-therapy. |
| first_indexed | 2024-12-10T16:05:54Z |
| format | Article |
| id | doaj.art-dff4ea687dc14cbc837f9a5a6e5464b7 |
| institution | Directory Open Access Journal |
| issn | 2314-8535 |
| language | English |
| last_indexed | 2024-12-10T16:05:54Z |
| publishDate | 2016-09-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-dff4ea687dc14cbc837f9a5a6e5464b72022-12-22T01:42:15ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85352016-09-015325527610.1016/j.bjbas.2016.07.001α-Mangostin Mediated Pharmacological Modulation of Hepatic Carbohydrate Metabolism in Diabetes Induced Wistar RatVikas Kumar0Prakash Chandra Bhatt1Gaurav Kaithwas2Mohd Rashid3F.A. Al-abbasi4Jalaluddin A.J. Khan5Firoz Anwar6Amita Verma7Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institute of Agriculture, Technology & Sciences, Allahabad, Uttar Pradesh 211007, IndiaCentre for Advanced Research in Pharmaceutical Sciences, Microbial and Pharmaceutical Biotechnology Laboratory, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, IndiaDepartment of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University (Central University), Vidya Vihar, Rai Bareli Road, Lucknow 226025, IndiaDepartment of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institute of Agriculture, Technology & Sciences, Allahabad, Uttar Pradesh 211007, IndiaDepartment of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institute of Agriculture, Technology & Sciences, Allahabad, Uttar Pradesh 211007, IndiaGarcinia mangostana L. (Fruit) has been commonly used as folklore drug in the treatment of various types of diseases. The present experiment was designed to evaluate the potential effect of α-mangostin mediated pharmacological modulation of hepatic carbohydrate metabolism in streptozotocin (STZ) induced diabetic rats. Oral glucose tolerance test (OGTT) was performed in normoglycemic rats. Single intraperitoneal injection of STZ (60 mg/kg, body weight) was used for induction the diabetes in Swiss albino (Wistar strain) rats. The rats were divided into different groups. Blood glucose level, body weight, insulin, glycated hemoglobin and hemoglobin levels were recorded at regular intervals. Biochemical parameters, liver enzymes, lipid profile, antioxidant parameters and inflammatory cytokine mediators were also scrutinized. Histopathology study of kidney, pancreas and liver were performed. The result of OGTT study depicted the better utilization of glucose in experimental rats. STZ induced diabetic rats treated with α-mangostin (25, 50 and 100 mg/kg, p.o.) and glibenclamide depicted the decline in the level of blood glucose; enhanced body weight and showed the better utilization of glucose by different organs. STZ induced diabetic rats treated with α-mangostin illustrated the increased level of plasma insulin, hemoglobin, hexokinase, HDL, total protein, SOD, CAT, GSH and declined level of glycated hemoglobin, fructose-1-6-biphosphatase, glucose-6-Phosphatase, TC, TG, LDL, VLDL, CRE, BUN, SGOT, SGPT, ALP and LPO at effective dose dependent manners. Histological study showed the inflamed blood vessels in diabetic kidney, which was less in α-mangostin treated rats; diabetic pancreatic showed the complete damage of β cells, islets, aciini and producing necrosis, but all damage was less obvious in α-mangostin treating group rats; diabetic liver showed the damage of hepatocytes as well as central vein but was less in treated groups. Considering the above results, α-mangostin shows potential to develop a medicine for diabetes, hyperlipidemia, renal and hepatic protection as combinational or mono-therapy.http://www.sciencedirect.com/science/article/pii/S2314853516300622α-mangostinTNF-αCRPIL-6StreptozotocinPancreasKidneyβ cells |
| spellingShingle | Vikas Kumar Prakash Chandra Bhatt Gaurav Kaithwas Mohd Rashid F.A. Al-abbasi Jalaluddin A.J. Khan Firoz Anwar Amita Verma α-Mangostin Mediated Pharmacological Modulation of Hepatic Carbohydrate Metabolism in Diabetes Induced Wistar Rat Beni-Suef University Journal of Basic and Applied Sciences α-mangostin TNF-α CRP IL-6 Streptozotocin Pancreas Kidney β cells |
| title | α-Mangostin Mediated Pharmacological Modulation of Hepatic Carbohydrate Metabolism in Diabetes Induced Wistar Rat |
| title_full | α-Mangostin Mediated Pharmacological Modulation of Hepatic Carbohydrate Metabolism in Diabetes Induced Wistar Rat |
| title_fullStr | α-Mangostin Mediated Pharmacological Modulation of Hepatic Carbohydrate Metabolism in Diabetes Induced Wistar Rat |
| title_full_unstemmed | α-Mangostin Mediated Pharmacological Modulation of Hepatic Carbohydrate Metabolism in Diabetes Induced Wistar Rat |
| title_short | α-Mangostin Mediated Pharmacological Modulation of Hepatic Carbohydrate Metabolism in Diabetes Induced Wistar Rat |
| title_sort | α mangostin mediated pharmacological modulation of hepatic carbohydrate metabolism in diabetes induced wistar rat |
| topic | α-mangostin TNF-α CRP IL-6 Streptozotocin Pancreas Kidney β cells |
| url | http://www.sciencedirect.com/science/article/pii/S2314853516300622 |
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