Anti-Inflammatory and Antiproliferative Properties of Sweet Cherry Phenolic-Rich Extracts
Cherries have largely been investigated due to their high content in phenolics in order to fully explore their health-promoting properties. Therefore, this work aimed to assess, for the first time, the anti-inflammatory potential of phenolic-targeted fractions of the <i>Saco</i> cherry,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/1/268 |
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| author | Ana C. Gonçalves Ana R. Costa José D. Flores-Félix Amílcar Falcão Gilberto Alves Luís R. Silva |
| author_facet | Ana C. Gonçalves Ana R. Costa José D. Flores-Félix Amílcar Falcão Gilberto Alves Luís R. Silva |
| author_sort | Ana C. Gonçalves |
| collection | DOAJ |
| description | Cherries have largely been investigated due to their high content in phenolics in order to fully explore their health-promoting properties. Therefore, this work aimed to assess, for the first time, the anti-inflammatory potential of phenolic-targeted fractions of the <i>Saco</i> cherry, using RAW 264.7 macrophages stimulated with lipopolysaccharide. Additionally, the cytotoxic effects on gastric adenocarcinoma (AGS), neuroblastoma (SH-SY5Y) and normal human dermal fibroblast (NHDF) cells were evaluated, as well as the ability to protect these cellular models against induced oxidative stress. The obtained data revealed that cherry fractions can interfere with cellular nitric oxide (NO) levels by capturing NO radicals and decreasing inducible nitric oxide synthase and cyclooxygenase-2 expression. Furthermore, it was observed that all cherry fractions exhibited dose-dependent cytotoxicity against AGS cells, presenting cytotoxic selectivity for these cancer cells when compared to SH-SY5Y and NHDF cells. Regarding their capacity to protect cancer cells against oxidative injury, in most assays, the total cherry extract was the most effective. Overall, this study reinforces the idea that sweet cherries can be incorporated into new pharmaceutical products, smart foods and nutraceuticals. |
| first_indexed | 2024-03-10T03:30:22Z |
| format | Article |
| id | doaj.art-dff5d86973aa4ce5a27327f1b2fbb60d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:30:22Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-dff5d86973aa4ce5a27327f1b2fbb60d2023-11-23T11:59:10ZengMDPI AGMolecules1420-30492022-01-0127126810.3390/molecules27010268Anti-Inflammatory and Antiproliferative Properties of Sweet Cherry Phenolic-Rich ExtractsAna C. Gonçalves0Ana R. Costa1José D. Flores-Félix2Amílcar Falcão3Gilberto Alves4Luís R. Silva5CICS-UBI—Health Sciences Research Centre, University of Beira Interior, 6201-001 Covilhã, PortugalCICS-UBI—Health Sciences Research Centre, University of Beira Interior, 6201-001 Covilhã, PortugalCICS-UBI—Health Sciences Research Centre, University of Beira Interior, 6201-001 Covilhã, PortugalCIBIT—Coimbra Institute for Biomedical Imaging and Translational Research, University of Coimbra, 3004-531 Coimbra, PortugalCICS-UBI—Health Sciences Research Centre, University of Beira Interior, 6201-001 Covilhã, PortugalCICS-UBI—Health Sciences Research Centre, University of Beira Interior, 6201-001 Covilhã, PortugalCherries have largely been investigated due to their high content in phenolics in order to fully explore their health-promoting properties. Therefore, this work aimed to assess, for the first time, the anti-inflammatory potential of phenolic-targeted fractions of the <i>Saco</i> cherry, using RAW 264.7 macrophages stimulated with lipopolysaccharide. Additionally, the cytotoxic effects on gastric adenocarcinoma (AGS), neuroblastoma (SH-SY5Y) and normal human dermal fibroblast (NHDF) cells were evaluated, as well as the ability to protect these cellular models against induced oxidative stress. The obtained data revealed that cherry fractions can interfere with cellular nitric oxide (NO) levels by capturing NO radicals and decreasing inducible nitric oxide synthase and cyclooxygenase-2 expression. Furthermore, it was observed that all cherry fractions exhibited dose-dependent cytotoxicity against AGS cells, presenting cytotoxic selectivity for these cancer cells when compared to SH-SY5Y and NHDF cells. Regarding their capacity to protect cancer cells against oxidative injury, in most assays, the total cherry extract was the most effective. Overall, this study reinforces the idea that sweet cherries can be incorporated into new pharmaceutical products, smart foods and nutraceuticals.https://www.mdpi.com/1420-3049/27/1/268anti-inflammatorycytotoxicityoxidative stressphenolic compoundssweet cherries |
| spellingShingle | Ana C. Gonçalves Ana R. Costa José D. Flores-Félix Amílcar Falcão Gilberto Alves Luís R. Silva Anti-Inflammatory and Antiproliferative Properties of Sweet Cherry Phenolic-Rich Extracts Molecules anti-inflammatory cytotoxicity oxidative stress phenolic compounds sweet cherries |
| title | Anti-Inflammatory and Antiproliferative Properties of Sweet Cherry Phenolic-Rich Extracts |
| title_full | Anti-Inflammatory and Antiproliferative Properties of Sweet Cherry Phenolic-Rich Extracts |
| title_fullStr | Anti-Inflammatory and Antiproliferative Properties of Sweet Cherry Phenolic-Rich Extracts |
| title_full_unstemmed | Anti-Inflammatory and Antiproliferative Properties of Sweet Cherry Phenolic-Rich Extracts |
| title_short | Anti-Inflammatory and Antiproliferative Properties of Sweet Cherry Phenolic-Rich Extracts |
| title_sort | anti inflammatory and antiproliferative properties of sweet cherry phenolic rich extracts |
| topic | anti-inflammatory cytotoxicity oxidative stress phenolic compounds sweet cherries |
| url | https://www.mdpi.com/1420-3049/27/1/268 |
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