Comparison of Serum Level and IL-18 Gene Expression and Reactive Protein in Patients with Type 2 Diabetes with Metabolic Syndrome and Healthy People
Introduction: Metabolic syndrome is a common clinical anomaly, with an increased risk of developing type 2 diabetes and cardiovascular risk factors. Increased inflammatory mediators, such as reactive protein,as well as the inflammatory cytokines can be associated with the incidence and progression o...
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| Format: | Article |
| Language: | fas |
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Ilam University of Medical Sciences
2019-09-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
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| Online Access: | http://sjimu.medilam.ac.ir/article-1-5113-en.html |
| _version_ | 1818667150114553856 |
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| author | leila Yadolahi Farsani Noosha Zia-jahromi Mohammad Vaezipour |
| author_facet | leila Yadolahi Farsani Noosha Zia-jahromi Mohammad Vaezipour |
| author_sort | leila Yadolahi Farsani |
| collection | DOAJ |
| description | Introduction: Metabolic syndrome is a common clinical anomaly, with an increased risk of developing type 2 diabetes and cardiovascular risk factors. Increased inflammatory mediators, such as reactive protein,as well as the inflammatory cytokines can be associated with the incidence and progression of metaboli syndrome and type 2 diabetes.
Materials & Methods: This study was performed on 35 male and female patients with an average age of 55 years, type 2 diabetes mellitus with metabolic syndrome and 35 healthy subjects as control group. Separated serum samples were kept at -20 ° C for quantitative evaluation of serum IL-18 by ELISA kit and C-reactive protein. RNA extraction from cells was performed by JenaBioscience kit and finally Gene expression assay was performed quantitatively using Real Time RTPCR.
Findings: The mean serum levels of IL-18 were significantly increased (P=0.008), and serum reactive protein (P<0.001) was significantly increased in patients with type 2 diabetes mellitus with metabolic syndrome compared with healthy subjects. Also, expression of IL-18 gene (P=0.003) in lymphocyte cells of type II diabetes patients with metabolic syndrome was significantly higher than healthy subjects.
Discussion & Conclusions: The present study showed that patients with type 2 diabetes mellitus with metabolic syndrome have high levels of inflammatory factors such as IL-18 in their serum. it can be concluded that serum levels of IL-18, C-reactive protein and IL-18 expression play an important role in inflammatory response and progression. Type 2 diabetes and metabolic syndrome. |
| first_indexed | 2024-12-17T06:15:51Z |
| format | Article |
| id | doaj.art-dffc398c289c4947a428591a36d073f8 |
| institution | Directory Open Access Journal |
| issn | 1563-4728 2588-3135 |
| language | fas |
| last_indexed | 2024-12-17T06:15:51Z |
| publishDate | 2019-09-01 |
| publisher | Ilam University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| spelling | doaj.art-dffc398c289c4947a428591a36d073f82022-12-21T22:00:31ZfasIlam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352019-09-01273173182Comparison of Serum Level and IL-18 Gene Expression and Reactive Protein in Patients with Type 2 Diabetes with Metabolic Syndrome and Healthy Peopleleila Yadolahi Farsani0Noosha Zia-jahromi1Mohammad Vaezipour2 Dept of Basic Science, Faculty of Biology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran Dept of Basic Science, Faculty of Biology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran Faculty of Medical Sciences, Shahid Beheshti University, Tehran, Iran Introduction: Metabolic syndrome is a common clinical anomaly, with an increased risk of developing type 2 diabetes and cardiovascular risk factors. Increased inflammatory mediators, such as reactive protein,as well as the inflammatory cytokines can be associated with the incidence and progression of metaboli syndrome and type 2 diabetes. Materials & Methods: This study was performed on 35 male and female patients with an average age of 55 years, type 2 diabetes mellitus with metabolic syndrome and 35 healthy subjects as control group. Separated serum samples were kept at -20 ° C for quantitative evaluation of serum IL-18 by ELISA kit and C-reactive protein. RNA extraction from cells was performed by JenaBioscience kit and finally Gene expression assay was performed quantitatively using Real Time RTPCR. Findings: The mean serum levels of IL-18 were significantly increased (P=0.008), and serum reactive protein (P<0.001) was significantly increased in patients with type 2 diabetes mellitus with metabolic syndrome compared with healthy subjects. Also, expression of IL-18 gene (P=0.003) in lymphocyte cells of type II diabetes patients with metabolic syndrome was significantly higher than healthy subjects. Discussion & Conclusions: The present study showed that patients with type 2 diabetes mellitus with metabolic syndrome have high levels of inflammatory factors such as IL-18 in their serum. it can be concluded that serum levels of IL-18, C-reactive protein and IL-18 expression play an important role in inflammatory response and progression. Type 2 diabetes and metabolic syndrome.http://sjimu.medilam.ac.ir/article-1-5113-en.htmlInterleukin 18C-reactive proteinType II diabetesMetabolic syndrome |
| spellingShingle | leila Yadolahi Farsani Noosha Zia-jahromi Mohammad Vaezipour Comparison of Serum Level and IL-18 Gene Expression and Reactive Protein in Patients with Type 2 Diabetes with Metabolic Syndrome and Healthy People Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām Interleukin 18 C-reactive protein Type II diabetes Metabolic syndrome |
| title | Comparison of Serum Level and IL-18 Gene Expression and Reactive Protein in Patients with Type 2 Diabetes with Metabolic
Syndrome and Healthy People |
| title_full | Comparison of Serum Level and IL-18 Gene Expression and Reactive Protein in Patients with Type 2 Diabetes with Metabolic
Syndrome and Healthy People |
| title_fullStr | Comparison of Serum Level and IL-18 Gene Expression and Reactive Protein in Patients with Type 2 Diabetes with Metabolic
Syndrome and Healthy People |
| title_full_unstemmed | Comparison of Serum Level and IL-18 Gene Expression and Reactive Protein in Patients with Type 2 Diabetes with Metabolic
Syndrome and Healthy People |
| title_short | Comparison of Serum Level and IL-18 Gene Expression and Reactive Protein in Patients with Type 2 Diabetes with Metabolic
Syndrome and Healthy People |
| title_sort | comparison of serum level and il 18 gene expression and reactive protein in patients with type 2 diabetes with metabolic syndrome and healthy people |
| topic | Interleukin 18 C-reactive protein Type II diabetes Metabolic syndrome |
| url | http://sjimu.medilam.ac.ir/article-1-5113-en.html |
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