Differential Vaginal Microbiota Profiling in Lactic-Acid-Producing Bacteria between Infertile Women with and without Chronic Endometritis
Purpose: Chronic endometritis (CE) is an infectious and inflammatory disorder associated with infertility of unknown etiology, repeated implantation failure, and recurrent pregnancy loss. In the current clinical practice, intrauterine interventions such as endometrial biopsy/histopathologic examinat...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/12/4/878 |
| _version_ | 1827621084921331712 |
|---|---|
| author | Suguru E. Tanaka Yoshiyuki Sakuraba Kotaro Kitaya Tomomoto Ishikawa |
| author_facet | Suguru E. Tanaka Yoshiyuki Sakuraba Kotaro Kitaya Tomomoto Ishikawa |
| author_sort | Suguru E. Tanaka |
| collection | DOAJ |
| description | Purpose: Chronic endometritis (CE) is an infectious and inflammatory disorder associated with infertility of unknown etiology, repeated implantation failure, and recurrent pregnancy loss. In the current clinical practice, intrauterine interventions such as endometrial biopsy/histopathologic examinations and/or hysteroscopy are required for the diagnosis of CE. In this study, we analyzed the microbiota in vaginal secretions (VS) as a potential prediction tool for CE in infertile women. Methods: Using next-generation sequencing analysis, we compared the VS and endometrial fluid (EF) microbiota in infertile women with (<i>n</i> = 20) or without CE (<i>n</i> = 103). Results: The detection rate of <i>Streptococcus</i> and <i>Enterococcus</i> as well as the bacterial abundance of <i>Atopobium</i> and <i>Bifidobacterium</i> in the VS microbiota was significantly lower in the CE group than in the non-CE group. Meanwhile, the detection rate and bacterial abundance of <i>Lactobacillus</i> in the EF and VS microbiota were at similar levels between the two groups. Conclusion: These findings suggest that VS microbiota in infertile women with CE is characterized by the reduction in <i>Bifidobacterium</i> and lactic-acid-producing bacteria other than <i>Lactobacillus</i>. Our results hold promise for the prediction of CE, not by somewhat interventional intrauterine procedures, but by less invasive VS sampling. TRIAL REGISTRATION NUMBER: UMIN000029449 (registration date 6 October 2017). |
| first_indexed | 2024-03-09T10:57:41Z |
| format | Article |
| id | doaj.art-dffedc08978041ec8ce2a2f1527534be |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T10:57:41Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-dffedc08978041ec8ce2a2f1527534be2023-12-01T01:32:18ZengMDPI AGDiagnostics2075-44182022-03-0112487810.3390/diagnostics12040878Differential Vaginal Microbiota Profiling in Lactic-Acid-Producing Bacteria between Infertile Women with and without Chronic EndometritisSuguru E. Tanaka0Yoshiyuki Sakuraba1Kotaro Kitaya2Tomomoto Ishikawa3Varinos Inc., DiverCity Tokyo Office Tower, 12F, 1-1-20 Aomi, Koto-ku, Tokyo 135-0064, JapanVarinos Inc., DiverCity Tokyo Office Tower, 12F, 1-1-20 Aomi, Koto-ku, Tokyo 135-0064, JapanReproduction Clinic Osaka, Grand Front Osaka Tower-A 15F, 4-20 Oofuka-cho, Kita-ku, Osaka 530-0011, JapanReproduction Clinic Osaka, Grand Front Osaka Tower-A 15F, 4-20 Oofuka-cho, Kita-ku, Osaka 530-0011, JapanPurpose: Chronic endometritis (CE) is an infectious and inflammatory disorder associated with infertility of unknown etiology, repeated implantation failure, and recurrent pregnancy loss. In the current clinical practice, intrauterine interventions such as endometrial biopsy/histopathologic examinations and/or hysteroscopy are required for the diagnosis of CE. In this study, we analyzed the microbiota in vaginal secretions (VS) as a potential prediction tool for CE in infertile women. Methods: Using next-generation sequencing analysis, we compared the VS and endometrial fluid (EF) microbiota in infertile women with (<i>n</i> = 20) or without CE (<i>n</i> = 103). Results: The detection rate of <i>Streptococcus</i> and <i>Enterococcus</i> as well as the bacterial abundance of <i>Atopobium</i> and <i>Bifidobacterium</i> in the VS microbiota was significantly lower in the CE group than in the non-CE group. Meanwhile, the detection rate and bacterial abundance of <i>Lactobacillus</i> in the EF and VS microbiota were at similar levels between the two groups. Conclusion: These findings suggest that VS microbiota in infertile women with CE is characterized by the reduction in <i>Bifidobacterium</i> and lactic-acid-producing bacteria other than <i>Lactobacillus</i>. Our results hold promise for the prediction of CE, not by somewhat interventional intrauterine procedures, but by less invasive VS sampling. TRIAL REGISTRATION NUMBER: UMIN000029449 (registration date 6 October 2017).https://www.mdpi.com/2075-4418/12/4/878chronic endometritisdysbiosislactic acid bacteriavaginal secretion microbiota |
| spellingShingle | Suguru E. Tanaka Yoshiyuki Sakuraba Kotaro Kitaya Tomomoto Ishikawa Differential Vaginal Microbiota Profiling in Lactic-Acid-Producing Bacteria between Infertile Women with and without Chronic Endometritis Diagnostics chronic endometritis dysbiosis lactic acid bacteria vaginal secretion microbiota |
| title | Differential Vaginal Microbiota Profiling in Lactic-Acid-Producing Bacteria between Infertile Women with and without Chronic Endometritis |
| title_full | Differential Vaginal Microbiota Profiling in Lactic-Acid-Producing Bacteria between Infertile Women with and without Chronic Endometritis |
| title_fullStr | Differential Vaginal Microbiota Profiling in Lactic-Acid-Producing Bacteria between Infertile Women with and without Chronic Endometritis |
| title_full_unstemmed | Differential Vaginal Microbiota Profiling in Lactic-Acid-Producing Bacteria between Infertile Women with and without Chronic Endometritis |
| title_short | Differential Vaginal Microbiota Profiling in Lactic-Acid-Producing Bacteria between Infertile Women with and without Chronic Endometritis |
| title_sort | differential vaginal microbiota profiling in lactic acid producing bacteria between infertile women with and without chronic endometritis |
| topic | chronic endometritis dysbiosis lactic acid bacteria vaginal secretion microbiota |
| url | https://www.mdpi.com/2075-4418/12/4/878 |
| work_keys_str_mv | AT suguruetanaka differentialvaginalmicrobiotaprofilinginlacticacidproducingbacteriabetweeninfertilewomenwithandwithoutchronicendometritis AT yoshiyukisakuraba differentialvaginalmicrobiotaprofilinginlacticacidproducingbacteriabetweeninfertilewomenwithandwithoutchronicendometritis AT kotarokitaya differentialvaginalmicrobiotaprofilinginlacticacidproducingbacteriabetweeninfertilewomenwithandwithoutchronicendometritis AT tomomotoishikawa differentialvaginalmicrobiotaprofilinginlacticacidproducingbacteriabetweeninfertilewomenwithandwithoutchronicendometritis |