Alteration of mitochondrial membrane potential (DELTA_PSI_m) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensis
The effects of the heavy metals cadmium and copper (50-500 ìM) on the apoptotic events involving changes in mitochondrial membrane potential (ÄØm) and phosphatidylserine (PS) translocation were investigated in the immune cells (celomocytes) of the earthworm Eisenia hortensis. Using the fluorescent p...
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| Format: | Article |
| Language: | English |
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University of Modena and Reggio Emilia
2011-06-01
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| Series: | Invertebrate Survival Journal |
| Subjects: | |
| Online Access: | http://www.isj.unimo.it/articoli/ISJ240.pdf |
| _version_ | 1818234171639726080 |
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| author | FM Bearoff SL Fuller-Espie |
| author_facet | FM Bearoff SL Fuller-Espie |
| author_sort | FM Bearoff |
| collection | DOAJ |
| description | The effects of the heavy metals cadmium and copper (50-500 ìM) on the apoptotic events involving changes in mitochondrial membrane potential (ÄØm) and phosphatidylserine (PS) translocation were investigated in the immune cells (celomocytes) of the earthworm Eisenia hortensis. Using the fluorescent probe JC-1, loss of membrane potential due to depolarization was detected in a greater proportion of cases when induced by cadmium compared to copper (58.7 % vs. 37 %) and at a lower concentration (50 ìM vs. 125ìM). With the use of the general caspase inhibitor Z-VAD-fmk, PS translocation detected by annexin V-FITC was found to be caspase-dependent when induced by cadmium at 125-250 ìM but not at 50 ìM or 500 ìM; a high proportion of earthworms (60 %) exhibited inhibitory effects. Additionally, the collapse in membrane potential and PS translocation were found to strongly correlate (r > 0.5) in 89 % of cases when induced by cadmium and copper. Thus, heavy metals appear to induce death in celomocytes of E. hortensis through apoptosis by means of caspase dependent pathways |
| first_indexed | 2024-12-12T11:33:50Z |
| format | Article |
| id | doaj.art-e0035ffdfb3244508a2203470d959e44 |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-12-12T11:33:50Z |
| publishDate | 2011-06-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-e0035ffdfb3244508a2203470d959e442022-12-22T00:25:43ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2011-06-018198108Alteration of mitochondrial membrane potential (DELTA_PSI_m) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensisFM BearoffSL Fuller-EspieThe effects of the heavy metals cadmium and copper (50-500 ìM) on the apoptotic events involving changes in mitochondrial membrane potential (ÄØm) and phosphatidylserine (PS) translocation were investigated in the immune cells (celomocytes) of the earthworm Eisenia hortensis. Using the fluorescent probe JC-1, loss of membrane potential due to depolarization was detected in a greater proportion of cases when induced by cadmium compared to copper (58.7 % vs. 37 %) and at a lower concentration (50 ìM vs. 125ìM). With the use of the general caspase inhibitor Z-VAD-fmk, PS translocation detected by annexin V-FITC was found to be caspase-dependent when induced by cadmium at 125-250 ìM but not at 50 ìM or 500 ìM; a high proportion of earthworms (60 %) exhibited inhibitory effects. Additionally, the collapse in membrane potential and PS translocation were found to strongly correlate (r > 0.5) in 89 % of cases when induced by cadmium and copper. Thus, heavy metals appear to induce death in celomocytes of E. hortensis through apoptosis by means of caspase dependent pathwayshttp://www.isj.unimo.it/articoli/ISJ240.pdfcadmium and copperJC-1annexin V-FITCmitochondrial membrane depolarizationphosphatidylserine translocationcaspases |
| spellingShingle | FM Bearoff SL Fuller-Espie Alteration of mitochondrial membrane potential (DELTA_PSI_m) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensis Invertebrate Survival Journal cadmium and copper JC-1 annexin V-FITC mitochondrial membrane depolarization phosphatidylserine translocation caspases |
| title | Alteration of mitochondrial membrane potential (DELTA_PSI_m) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensis |
| title_full | Alteration of mitochondrial membrane potential (DELTA_PSI_m) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensis |
| title_fullStr | Alteration of mitochondrial membrane potential (DELTA_PSI_m) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensis |
| title_full_unstemmed | Alteration of mitochondrial membrane potential (DELTA_PSI_m) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensis |
| title_short | Alteration of mitochondrial membrane potential (DELTA_PSI_m) and phosphatidylserine translocation as early indicators of heavy metal-induced apoptosis in the earthworm Eisenia hortensis |
| title_sort | alteration of mitochondrial membrane potential delta psi m and phosphatidylserine translocation as early indicators of heavy metal induced apoptosis in the earthworm eisenia hortensis |
| topic | cadmium and copper JC-1 annexin V-FITC mitochondrial membrane depolarization phosphatidylserine translocation caspases |
| url | http://www.isj.unimo.it/articoli/ISJ240.pdf |
| work_keys_str_mv | AT fmbearoff alterationofmitochondrialmembranepotentialdeltapsimandphosphatidylserinetranslocationasearlyindicatorsofheavymetalinducedapoptosisintheearthwormeiseniahortensis AT slfullerespie alterationofmitochondrialmembranepotentialdeltapsimandphosphatidylserinetranslocationasearlyindicatorsofheavymetalinducedapoptosisintheearthwormeiseniahortensis |