Association Analysis for Some Biochemical Traits in Wild Relatives of Wheat under Drought Stress Conditions
In the present study, we estimated genetic diversity and population structure in 186 accessions of <i>Triticum</i> and <i>Aegilops</i> species using 24 simple sequence repeat markers (SSR). Furthermore, an association analysis was performed for antioxidant activities, includi...
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MDPI AG
2022-08-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/8/1491 |
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| author | Alireza Pour-Aboughadareh Omid Jadidi Lia Shooshtari Peter Poczai Ali Ashraf Mehrabi |
| author_facet | Alireza Pour-Aboughadareh Omid Jadidi Lia Shooshtari Peter Poczai Ali Ashraf Mehrabi |
| author_sort | Alireza Pour-Aboughadareh |
| collection | DOAJ |
| description | In the present study, we estimated genetic diversity and population structure in 186 accessions of <i>Triticum</i> and <i>Aegilops</i> species using 24 simple sequence repeat markers (SSR). Furthermore, an association analysis was performed for antioxidant activities, including guaiacol peroxidase (GPX), ascorbate peroxidase (APX), peroxidase (POX), catalase (CAT), and dry matter (DM) under two control and drought stress conditions. Our findings showed that drought treatment significantly decreased DM, whereas activities of all antioxidant enzymes were increased compared to the control conditions. The results of correlation analysis indicated that, under drought stress conditions, all biochemical traits had a positive and significant association with each other and with dry matter. In the molecular section, the results of the analysis of molecular variance (AMOVA) indicated that the molecular variation within species is more than within them. The dendrogram obtained by cluster analysis showed that grouping the investigated accessions was in accordance with their genomic constitutions. The results of association analysis revealed 8 and 9 significant marker–trait associations (MTA) under control and drought stress conditions, respectively. Among identified MTAs, two associations were simultaneously found in both growing conditions. Moreover, several SSR markers were associated with multiple traits across both conditions. In conclusion, our results could provide worthwhile information regarding marker-assisted selection for the activity of antioxidant enzymes in future breeding programs. |
| first_indexed | 2024-03-09T09:56:46Z |
| format | Article |
| id | doaj.art-e00508a97b9a438ea97550e27b0118d4 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T09:56:46Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-e00508a97b9a438ea97550e27b0118d42023-12-01T23:44:39ZengMDPI AGGenes2073-44252022-08-01138149110.3390/genes13081491Association Analysis for Some Biochemical Traits in Wild Relatives of Wheat under Drought Stress ConditionsAlireza Pour-Aboughadareh0Omid Jadidi1Lia Shooshtari2Peter Poczai3Ali Ashraf Mehrabi4Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj P.O. Box 31587-77871, IranDepartment of Plant Breeding and Biotechnology, Science and Research Branch, Islamic Azad University, Tehran P.O. Box 14515/775, IranDepartment of Plant Breeding and Biotechnology, Kermanshah Branch, Islamic Azad University, Kermanshah P.O. Box 67146, IranBotany Unit, Finnish Museum of Natural History, University of Helsinki, P.O. Box 7, FI-00014 Helsinki, FinlandDepartment of Plant Biotechnology, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran P.O. Box 14968-13111, IranIn the present study, we estimated genetic diversity and population structure in 186 accessions of <i>Triticum</i> and <i>Aegilops</i> species using 24 simple sequence repeat markers (SSR). Furthermore, an association analysis was performed for antioxidant activities, including guaiacol peroxidase (GPX), ascorbate peroxidase (APX), peroxidase (POX), catalase (CAT), and dry matter (DM) under two control and drought stress conditions. Our findings showed that drought treatment significantly decreased DM, whereas activities of all antioxidant enzymes were increased compared to the control conditions. The results of correlation analysis indicated that, under drought stress conditions, all biochemical traits had a positive and significant association with each other and with dry matter. In the molecular section, the results of the analysis of molecular variance (AMOVA) indicated that the molecular variation within species is more than within them. The dendrogram obtained by cluster analysis showed that grouping the investigated accessions was in accordance with their genomic constitutions. The results of association analysis revealed 8 and 9 significant marker–trait associations (MTA) under control and drought stress conditions, respectively. Among identified MTAs, two associations were simultaneously found in both growing conditions. Moreover, several SSR markers were associated with multiple traits across both conditions. In conclusion, our results could provide worthwhile information regarding marker-assisted selection for the activity of antioxidant enzymes in future breeding programs.https://www.mdpi.com/2073-4425/13/8/1491genetic resources<i>Aegilops</i> spp.SSR markersbiochemical traitswheat |
| spellingShingle | Alireza Pour-Aboughadareh Omid Jadidi Lia Shooshtari Peter Poczai Ali Ashraf Mehrabi Association Analysis for Some Biochemical Traits in Wild Relatives of Wheat under Drought Stress Conditions Genes genetic resources <i>Aegilops</i> spp. SSR markers biochemical traits wheat |
| title | Association Analysis for Some Biochemical Traits in Wild Relatives of Wheat under Drought Stress Conditions |
| title_full | Association Analysis for Some Biochemical Traits in Wild Relatives of Wheat under Drought Stress Conditions |
| title_fullStr | Association Analysis for Some Biochemical Traits in Wild Relatives of Wheat under Drought Stress Conditions |
| title_full_unstemmed | Association Analysis for Some Biochemical Traits in Wild Relatives of Wheat under Drought Stress Conditions |
| title_short | Association Analysis for Some Biochemical Traits in Wild Relatives of Wheat under Drought Stress Conditions |
| title_sort | association analysis for some biochemical traits in wild relatives of wheat under drought stress conditions |
| topic | genetic resources <i>Aegilops</i> spp. SSR markers biochemical traits wheat |
| url | https://www.mdpi.com/2073-4425/13/8/1491 |
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