Genome-wide linkage mapping of root system architecture-related traits in common wheat (Triticum aestivum L.)
Identifying loci for root system architecture (RSA) traits and developing available markers are crucial for wheat breeding. In this study, RSA-related traits, including total root length (TRL), total root area (TRA), and number of root tips (NRT), were evaluated in the Doumai/Shi4185 recombinant inb...
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1274392/full |
| _version_ | 1797660678940524544 |
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| author | Yirong Jin Yamei Wang Jindong Liu Fuyan Wang Xiaodong Qiu Peng Liu |
| author_facet | Yirong Jin Yamei Wang Jindong Liu Fuyan Wang Xiaodong Qiu Peng Liu |
| author_sort | Yirong Jin |
| collection | DOAJ |
| description | Identifying loci for root system architecture (RSA) traits and developing available markers are crucial for wheat breeding. In this study, RSA-related traits, including total root length (TRL), total root area (TRA), and number of root tips (NRT), were evaluated in the Doumai/Shi4185 recombinant inbred line (RIL) population under hydroponics. In addition, both the RILs and parents were genotyped using the wheat 90K single-nucleotide polymorphism (SNP) array. In total, two quantitative trait loci (QTLs) each for TRL (QTRL.caas-4A.1 and QTRL.caas-4A.2), TRA (QTRA.caas-4A and QTRA.caas-4D), and NRT (QNRT.caas-5B and QNRT.caas-5D) were identified and each explaining 5.94%–9.47%, 6.85%–7.10%, and 5.91%–10.16% phenotypic variances, respectively. Among these, QTRL.caas-4A.1 and QTRA.caas-4A overlapped with previous reports, while QTRL.caas-4A.2, QTRA.caas-4D, QNRT.caas-5B, and QNRT.caas-5D were novel. The favorable alleles of QTRL.caas-4A.1, QTRA.caas-4A, and QTRA.caas-5B were contributed by Doumai, whereas the favorable alleles of QTRL.caas-4A.2, QTRA.caas-4D, and QTRA.caas-5D originated from Shi 4185. Additionally, two competitive allele-specific PCR (KASP) markers, Kasp_4A_RL (QTRA.caas-4A) and Kasp_5D_RT (QNRT.caas-5D), were developed and validated in 165 wheat accessions. This study provides new loci and available KASP markers, accelerating wheat breeding for higher yields. |
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| id | doaj.art-e0083c808fdf42e996d28deee8bac2b8 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-11T18:34:23Z |
| publishDate | 2023-10-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-e0083c808fdf42e996d28deee8bac2b82023-10-13T07:55:46ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-10-011410.3389/fpls.2023.12743921274392Genome-wide linkage mapping of root system architecture-related traits in common wheat (Triticum aestivum L.)Yirong Jin0Yamei Wang1Jindong Liu2Fuyan Wang3Xiaodong Qiu4Peng Liu5Wheat Research Institute, Dezhou Academy of Agricultural Sciences, Dezhou, ChinaSchool of Agriculture, Sun Yat-sen University, Shenzhen, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaWheat Research Institute, Dezhou Academy of Agricultural Sciences, Dezhou, ChinaDepartment of Science and Technology of Shandong Province, Jinan, ChinaWheat Research Institute, Dezhou Academy of Agricultural Sciences, Dezhou, ChinaIdentifying loci for root system architecture (RSA) traits and developing available markers are crucial for wheat breeding. In this study, RSA-related traits, including total root length (TRL), total root area (TRA), and number of root tips (NRT), were evaluated in the Doumai/Shi4185 recombinant inbred line (RIL) population under hydroponics. In addition, both the RILs and parents were genotyped using the wheat 90K single-nucleotide polymorphism (SNP) array. In total, two quantitative trait loci (QTLs) each for TRL (QTRL.caas-4A.1 and QTRL.caas-4A.2), TRA (QTRA.caas-4A and QTRA.caas-4D), and NRT (QNRT.caas-5B and QNRT.caas-5D) were identified and each explaining 5.94%–9.47%, 6.85%–7.10%, and 5.91%–10.16% phenotypic variances, respectively. Among these, QTRL.caas-4A.1 and QTRA.caas-4A overlapped with previous reports, while QTRL.caas-4A.2, QTRA.caas-4D, QNRT.caas-5B, and QNRT.caas-5D were novel. The favorable alleles of QTRL.caas-4A.1, QTRA.caas-4A, and QTRA.caas-5B were contributed by Doumai, whereas the favorable alleles of QTRL.caas-4A.2, QTRA.caas-4D, and QTRA.caas-5D originated from Shi 4185. Additionally, two competitive allele-specific PCR (KASP) markers, Kasp_4A_RL (QTRA.caas-4A) and Kasp_5D_RT (QNRT.caas-5D), were developed and validated in 165 wheat accessions. This study provides new loci and available KASP markers, accelerating wheat breeding for higher yields.https://www.frontiersin.org/articles/10.3389/fpls.2023.1274392/fullbread wheatmarker-assisted selectionquantitative trait locus (QTL)root system architecture (RSA)single-nucleotide polymorphism (SNP) |
| spellingShingle | Yirong Jin Yamei Wang Jindong Liu Fuyan Wang Xiaodong Qiu Peng Liu Genome-wide linkage mapping of root system architecture-related traits in common wheat (Triticum aestivum L.) Frontiers in Plant Science bread wheat marker-assisted selection quantitative trait locus (QTL) root system architecture (RSA) single-nucleotide polymorphism (SNP) |
| title | Genome-wide linkage mapping of root system architecture-related traits in common wheat (Triticum aestivum L.) |
| title_full | Genome-wide linkage mapping of root system architecture-related traits in common wheat (Triticum aestivum L.) |
| title_fullStr | Genome-wide linkage mapping of root system architecture-related traits in common wheat (Triticum aestivum L.) |
| title_full_unstemmed | Genome-wide linkage mapping of root system architecture-related traits in common wheat (Triticum aestivum L.) |
| title_short | Genome-wide linkage mapping of root system architecture-related traits in common wheat (Triticum aestivum L.) |
| title_sort | genome wide linkage mapping of root system architecture related traits in common wheat triticum aestivum l |
| topic | bread wheat marker-assisted selection quantitative trait locus (QTL) root system architecture (RSA) single-nucleotide polymorphism (SNP) |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1274392/full |
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