Denatured proteins show new vitality: Green synthesis of germanium oxide hollow microspheres with versatile functions by denaturing proteins around bubbles
Abstract The hollow structure has long attracted great attention because of its excellent properties. However, this special structure is usually synthesized through some complex approaches. Herein, we discovered that denatured bovine serum albumin (BSA) can trigger unusual biomineralization for the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-02-01
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| Series: | Aggregate |
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| Online Access: | https://doi.org/10.1002/agt2.204 |
| _version_ | 1797902607650390016 |
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| author | Yanfang Zhao Xin Liang Daoyuan Chen Xuhui Bian Wanjian Liu Lei Han |
| author_facet | Yanfang Zhao Xin Liang Daoyuan Chen Xuhui Bian Wanjian Liu Lei Han |
| author_sort | Yanfang Zhao |
| collection | DOAJ |
| description | Abstract The hollow structure has long attracted great attention because of its excellent properties. However, this special structure is usually synthesized through some complex approaches. Herein, we discovered that denatured bovine serum albumin (BSA) can trigger unusual biomineralization for the simple, green and shape‐controllable synthesis of germanium oxide (GeOx) hollow microsphere (HMS). At high temperature (60°C), BSA was denatured, and a compact BSA layer was formed around the H2 bubbles. The denatured BSA layer was stable and suitable for anchoring and growing GeOx. By simply changing the BSA concentration and temperature, various morphologies of GeOx could be obtained. Due to the denatured protein skeletons and microenvironment‐regulated collapse, GeOx HMS showed great potential for intelligently responsive pesticide delivery in the insect gut, showing superiority over traditional delivery systems, which early release pesticides in the mouth and stomach. Inspired by its large specific surface area, excellent biocompatibility, modifiable functional groups, and high electrocatalytic activity, GeOx HMS was also applied to versatile sensors for H2O2 assays at physiological pH and rapid coronavirus COVID‐19 detection. This work not only provides some evidence for understanding proteins in depth but also paves a new avenue for the biomineralization‐inspired synthesis of hollow structures with versatile functions. |
| first_indexed | 2024-04-10T09:20:19Z |
| format | Article |
| id | doaj.art-e00908d759b44f7f8527e10f6e55eade |
| institution | Directory Open Access Journal |
| issn | 2692-4560 |
| language | English |
| last_indexed | 2024-04-10T09:20:19Z |
| publishDate | 2023-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aggregate |
| spelling | doaj.art-e00908d759b44f7f8527e10f6e55eade2023-02-20T15:03:57ZengWileyAggregate2692-45602023-02-0141n/an/a10.1002/agt2.204Denatured proteins show new vitality: Green synthesis of germanium oxide hollow microspheres with versatile functions by denaturing proteins around bubblesYanfang Zhao0Xin Liang1Daoyuan Chen2Xuhui Bian3Wanjian Liu4Lei Han5College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural University Qingdao Shandong ChinaCollege of Chemistry and Pharmaceutical Sciences Qingdao Agricultural University Qingdao Shandong ChinaCollege of Chemistry and Pharmaceutical Sciences Qingdao Agricultural University Qingdao Shandong ChinaCollege of Chemistry and Pharmaceutical Sciences Qingdao Agricultural University Qingdao Shandong ChinaQingdao Shuojing Biological Technology Co., Ltd. Qingdao Shandong ChinaCollege of Chemistry and Pharmaceutical Sciences Qingdao Agricultural University Qingdao Shandong ChinaAbstract The hollow structure has long attracted great attention because of its excellent properties. However, this special structure is usually synthesized through some complex approaches. Herein, we discovered that denatured bovine serum albumin (BSA) can trigger unusual biomineralization for the simple, green and shape‐controllable synthesis of germanium oxide (GeOx) hollow microsphere (HMS). At high temperature (60°C), BSA was denatured, and a compact BSA layer was formed around the H2 bubbles. The denatured BSA layer was stable and suitable for anchoring and growing GeOx. By simply changing the BSA concentration and temperature, various morphologies of GeOx could be obtained. Due to the denatured protein skeletons and microenvironment‐regulated collapse, GeOx HMS showed great potential for intelligently responsive pesticide delivery in the insect gut, showing superiority over traditional delivery systems, which early release pesticides in the mouth and stomach. Inspired by its large specific surface area, excellent biocompatibility, modifiable functional groups, and high electrocatalytic activity, GeOx HMS was also applied to versatile sensors for H2O2 assays at physiological pH and rapid coronavirus COVID‐19 detection. This work not only provides some evidence for understanding proteins in depth but also paves a new avenue for the biomineralization‐inspired synthesis of hollow structures with versatile functions.https://doi.org/10.1002/agt2.204biomineralizationCOVID‐19denatured proteinelectrochemical sensorhollow microsphereintelligent pesticide delivery |
| spellingShingle | Yanfang Zhao Xin Liang Daoyuan Chen Xuhui Bian Wanjian Liu Lei Han Denatured proteins show new vitality: Green synthesis of germanium oxide hollow microspheres with versatile functions by denaturing proteins around bubbles Aggregate biomineralization COVID‐19 denatured protein electrochemical sensor hollow microsphere intelligent pesticide delivery |
| title | Denatured proteins show new vitality: Green synthesis of germanium oxide hollow microspheres with versatile functions by denaturing proteins around bubbles |
| title_full | Denatured proteins show new vitality: Green synthesis of germanium oxide hollow microspheres with versatile functions by denaturing proteins around bubbles |
| title_fullStr | Denatured proteins show new vitality: Green synthesis of germanium oxide hollow microspheres with versatile functions by denaturing proteins around bubbles |
| title_full_unstemmed | Denatured proteins show new vitality: Green synthesis of germanium oxide hollow microspheres with versatile functions by denaturing proteins around bubbles |
| title_short | Denatured proteins show new vitality: Green synthesis of germanium oxide hollow microspheres with versatile functions by denaturing proteins around bubbles |
| title_sort | denatured proteins show new vitality green synthesis of germanium oxide hollow microspheres with versatile functions by denaturing proteins around bubbles |
| topic | biomineralization COVID‐19 denatured protein electrochemical sensor hollow microsphere intelligent pesticide delivery |
| url | https://doi.org/10.1002/agt2.204 |
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