Effects of intravenous administration of ascorbic acid (vitamin C) on oxidative status in healthy adult horses
Abstract Background Ascorbic acid (AA) is an antioxidant that might be beneficial for adjunctive treatment of sepsis in horses. The optimal dose and effects on oxidative status are unknown. Hypothesis Ascorbic acid administration will increase plasma AA concentrations and decrease determinants of re...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.16934 |
| _version_ | 1797349540464951296 |
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| author | Sandra D. Taylor Kelsey A. Hart Sarah Vaughn Shyla C. Giancola Priscila B. S. Serpa Andrea P. Santos |
| author_facet | Sandra D. Taylor Kelsey A. Hart Sarah Vaughn Shyla C. Giancola Priscila B. S. Serpa Andrea P. Santos |
| author_sort | Sandra D. Taylor |
| collection | DOAJ |
| description | Abstract Background Ascorbic acid (AA) is an antioxidant that might be beneficial for adjunctive treatment of sepsis in horses. The optimal dose and effects on oxidative status are unknown. Hypothesis Ascorbic acid administration will increase plasma AA concentrations and decrease determinants of reactive oxygen metabolites (dROM), basal and stimulant‐induced intraerythrocytic reactive oxygen species (ROS) concentrations, and stimulant‐induced neutrophil ROS production, and increase plasma antioxidant capacity (PAC) in a dose‐dependent manner. Animals Eight healthy horses. Methods Randomized placebo‐controlled crossover study. Each horse received 4 single‐dose IV treatments including AA at 25, 50, and 100 mg/kg and saline (placebo) with each treatment separated by ≥1 week. Blood was collected at baseline, 2 and 6 hours for assessment of plasma dROM and PAC via photometer, intraerythrocytic ROS by flow cytometry, and stimulant‐induced neutrophil ROS by a fluorometric assay. Plasma AA concentrations were measured by high‐performance liquid chromatography/electrochemical detection. Results Ascorbic acid at 100 mg/kg resulted in decreased dROM 2 hours after treatment (P = .03, 95% CI 5.51‐121.2, point estimate 63.3). There was no effect of AA on basal or stimulant‐induced intraerythrocytic ROS (P = .88, 95% CI −0.156 to 0.081, point estimate −0.037; P = .93, 95% CI −0.123 to 0.112, point estimate −0.006, respectively), basal or stimulant‐induced neutrophil ROS (P ≥ .12, 95% CI −644.9 to 56.2, point estimate −294.4), or PAC (P ≥ .64, 95% CI −1567 to 463.4, point estimate −552.0) at any dose or timepoint. Plasma AA concentrations increased in a dose‐dependent manner. Conclusions and Clinical Importance High‐dose administration of AA might provide antioxidant benefits in horses. |
| first_indexed | 2024-03-08T12:31:44Z |
| format | Article |
| id | doaj.art-e00c99f7e29b48358232a35ba93fcbc3 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-03-08T12:31:44Z |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-e00c99f7e29b48358232a35ba93fcbc32024-01-22T02:05:50ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762024-01-0138146046810.1111/jvim.16934Effects of intravenous administration of ascorbic acid (vitamin C) on oxidative status in healthy adult horsesSandra D. Taylor0Kelsey A. Hart1Sarah Vaughn2Shyla C. Giancola3Priscila B. S. Serpa4Andrea P. Santos5Department of Veterinary Clinical Sciences College of Veterinary Medicine, Purdue University West Lafayette Indiana USADepartment of Large Animal Medicine, College of Veterinary Medicine University of Georgia Athens Georgia USADepartment of Large Animal Medicine, College of Veterinary Medicine University of Georgia Athens Georgia USADepartment of Large Animal Medicine, College of Veterinary Medicine University of Georgia Athens Georgia USADepartment of Comparative Pathobiology, College of Veterinary Medicine Purdue University West Lafayette Indiana USADepartment of Comparative Pathobiology, College of Veterinary Medicine Purdue University West Lafayette Indiana USAAbstract Background Ascorbic acid (AA) is an antioxidant that might be beneficial for adjunctive treatment of sepsis in horses. The optimal dose and effects on oxidative status are unknown. Hypothesis Ascorbic acid administration will increase plasma AA concentrations and decrease determinants of reactive oxygen metabolites (dROM), basal and stimulant‐induced intraerythrocytic reactive oxygen species (ROS) concentrations, and stimulant‐induced neutrophil ROS production, and increase plasma antioxidant capacity (PAC) in a dose‐dependent manner. Animals Eight healthy horses. Methods Randomized placebo‐controlled crossover study. Each horse received 4 single‐dose IV treatments including AA at 25, 50, and 100 mg/kg and saline (placebo) with each treatment separated by ≥1 week. Blood was collected at baseline, 2 and 6 hours for assessment of plasma dROM and PAC via photometer, intraerythrocytic ROS by flow cytometry, and stimulant‐induced neutrophil ROS by a fluorometric assay. Plasma AA concentrations were measured by high‐performance liquid chromatography/electrochemical detection. Results Ascorbic acid at 100 mg/kg resulted in decreased dROM 2 hours after treatment (P = .03, 95% CI 5.51‐121.2, point estimate 63.3). There was no effect of AA on basal or stimulant‐induced intraerythrocytic ROS (P = .88, 95% CI −0.156 to 0.081, point estimate −0.037; P = .93, 95% CI −0.123 to 0.112, point estimate −0.006, respectively), basal or stimulant‐induced neutrophil ROS (P ≥ .12, 95% CI −644.9 to 56.2, point estimate −294.4), or PAC (P ≥ .64, 95% CI −1567 to 463.4, point estimate −552.0) at any dose or timepoint. Plasma AA concentrations increased in a dose‐dependent manner. Conclusions and Clinical Importance High‐dose administration of AA might provide antioxidant benefits in horses.https://doi.org/10.1111/jvim.16934equineoxidative stressreactive oxygen speciessepsis |
| spellingShingle | Sandra D. Taylor Kelsey A. Hart Sarah Vaughn Shyla C. Giancola Priscila B. S. Serpa Andrea P. Santos Effects of intravenous administration of ascorbic acid (vitamin C) on oxidative status in healthy adult horses Journal of Veterinary Internal Medicine equine oxidative stress reactive oxygen species sepsis |
| title | Effects of intravenous administration of ascorbic acid (vitamin C) on oxidative status in healthy adult horses |
| title_full | Effects of intravenous administration of ascorbic acid (vitamin C) on oxidative status in healthy adult horses |
| title_fullStr | Effects of intravenous administration of ascorbic acid (vitamin C) on oxidative status in healthy adult horses |
| title_full_unstemmed | Effects of intravenous administration of ascorbic acid (vitamin C) on oxidative status in healthy adult horses |
| title_short | Effects of intravenous administration of ascorbic acid (vitamin C) on oxidative status in healthy adult horses |
| title_sort | effects of intravenous administration of ascorbic acid vitamin c on oxidative status in healthy adult horses |
| topic | equine oxidative stress reactive oxygen species sepsis |
| url | https://doi.org/10.1111/jvim.16934 |
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