Formulation and Characterization of a Methacrylated Chitosan Topical Treatment with Dispersed Magnetite Nanoparticles Functionalized with Hydrophobic Drugs Encapsulated in Liposomes
Cutaneous administration has advantages over the oral or intravenous route, such as convenience for the patient, avoiding hepatic metabolism, and providing sustained administration of the active component over long periods of time. A major challenge in this route is the administration of drugs that...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Biology and Life Sciences Forum |
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| Online Access: | https://www.mdpi.com/2673-9976/20/1/27 |
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| author | Monica Gantiva-Díaz Juan C. Cruz Carolina Muñoz-Camargo |
| author_facet | Monica Gantiva-Díaz Juan C. Cruz Carolina Muñoz-Camargo |
| author_sort | Monica Gantiva-Díaz |
| collection | DOAJ |
| description | Cutaneous administration has advantages over the oral or intravenous route, such as convenience for the patient, avoiding hepatic metabolism, and providing sustained administration of the active component over long periods of time. A major challenge in this route is the administration of drugs that are difficult to penetrate. For these, it is necessary to design delivery vehicles that help increase the stability of the active components and facilitate transport across the skin barrier. In this work, magnetoliposomes (MLPs) immobilizing magnetite nanoparticles (MNPs) have been realized. MNPs act as a nanocarrier for hydrophobic drugs, such as doxorubicin (DOX). To facilitate topical application, MLPs were dispersed in photoresponsive methacrylated chitosan hydrogels. For this purpose, the MLPs were synthesized by coprecipitation of FeCl<sub>3</sub> and FeCl<sub>2</sub>. Subsequently, they were silanized and functionalized by a PEG spacer to bind DOX. The success of each functionalization step was evaluated by Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). The size and morphology of the PEG-DOX-MNPs were analyzed by DLS and TEM. Then, the MNPs-PEG-DOX MNPs were encapsulated in liposomes synthesized by the layer hydration method. Dispersion of MLPs in the hydrogel, followed by crosslinking with visible blue light, was performed. Preliminary FTIR results indicate a correct synthesis and functionalization of the MNPs, as indicated by the presence of bands corresponding to the Si-O stretching vibration at 1029 cm<sup>−1</sup> and Fe-O absorption bands around 560 cm<sup>−1</sup>. TGA results showed a weight loss of 3.5% for MNPs from 200 to 400 °C, which was attributed to silane ligands. The hydrodynamic diameter of the MNPs was 140 nm with polydispersity indices of 0.16. In a future work, DOX will be conjugated to MNPs and MLPs will be synthesized for dispersion in the hydrogel. Subsequently, drug release kinetics tests will be performed under relevant conditions. |
| first_indexed | 2024-03-11T06:52:01Z |
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| institution | Directory Open Access Journal |
| issn | 2673-9976 |
| language | English |
| last_indexed | 2024-03-11T06:52:01Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology and Life Sciences Forum |
| spelling | doaj.art-e011e2d9b2174d979efa4ef4795f12b32023-11-17T09:55:50ZengMDPI AGBiology and Life Sciences Forum2673-99762022-11-012012710.3390/IECBM2022-13509Formulation and Characterization of a Methacrylated Chitosan Topical Treatment with Dispersed Magnetite Nanoparticles Functionalized with Hydrophobic Drugs Encapsulated in LiposomesMonica Gantiva-Díaz0Juan C. Cruz1Carolina Muñoz-Camargo2Department of Biomedical Engineering, Universidad de los Andes, Bogotá 111711, ColombiaDepartment of Biomedical Engineering, Universidad de los Andes, Bogotá 111711, ColombiaDepartment of Biomedical Engineering, Universidad de los Andes, Bogotá 111711, ColombiaCutaneous administration has advantages over the oral or intravenous route, such as convenience for the patient, avoiding hepatic metabolism, and providing sustained administration of the active component over long periods of time. A major challenge in this route is the administration of drugs that are difficult to penetrate. For these, it is necessary to design delivery vehicles that help increase the stability of the active components and facilitate transport across the skin barrier. In this work, magnetoliposomes (MLPs) immobilizing magnetite nanoparticles (MNPs) have been realized. MNPs act as a nanocarrier for hydrophobic drugs, such as doxorubicin (DOX). To facilitate topical application, MLPs were dispersed in photoresponsive methacrylated chitosan hydrogels. For this purpose, the MLPs were synthesized by coprecipitation of FeCl<sub>3</sub> and FeCl<sub>2</sub>. Subsequently, they were silanized and functionalized by a PEG spacer to bind DOX. The success of each functionalization step was evaluated by Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). The size and morphology of the PEG-DOX-MNPs were analyzed by DLS and TEM. Then, the MNPs-PEG-DOX MNPs were encapsulated in liposomes synthesized by the layer hydration method. Dispersion of MLPs in the hydrogel, followed by crosslinking with visible blue light, was performed. Preliminary FTIR results indicate a correct synthesis and functionalization of the MNPs, as indicated by the presence of bands corresponding to the Si-O stretching vibration at 1029 cm<sup>−1</sup> and Fe-O absorption bands around 560 cm<sup>−1</sup>. TGA results showed a weight loss of 3.5% for MNPs from 200 to 400 °C, which was attributed to silane ligands. The hydrodynamic diameter of the MNPs was 140 nm with polydispersity indices of 0.16. In a future work, DOX will be conjugated to MNPs and MLPs will be synthesized for dispersion in the hydrogel. Subsequently, drug release kinetics tests will be performed under relevant conditions.https://www.mdpi.com/2673-9976/20/1/27magnetoliposomesnanoparticlesmagnetiteskin drug deliveryChiMa hydrogels |
| spellingShingle | Monica Gantiva-Díaz Juan C. Cruz Carolina Muñoz-Camargo Formulation and Characterization of a Methacrylated Chitosan Topical Treatment with Dispersed Magnetite Nanoparticles Functionalized with Hydrophobic Drugs Encapsulated in Liposomes Biology and Life Sciences Forum magnetoliposomes nanoparticles magnetite skin drug delivery ChiMa hydrogels |
| title | Formulation and Characterization of a Methacrylated Chitosan Topical Treatment with Dispersed Magnetite Nanoparticles Functionalized with Hydrophobic Drugs Encapsulated in Liposomes |
| title_full | Formulation and Characterization of a Methacrylated Chitosan Topical Treatment with Dispersed Magnetite Nanoparticles Functionalized with Hydrophobic Drugs Encapsulated in Liposomes |
| title_fullStr | Formulation and Characterization of a Methacrylated Chitosan Topical Treatment with Dispersed Magnetite Nanoparticles Functionalized with Hydrophobic Drugs Encapsulated in Liposomes |
| title_full_unstemmed | Formulation and Characterization of a Methacrylated Chitosan Topical Treatment with Dispersed Magnetite Nanoparticles Functionalized with Hydrophobic Drugs Encapsulated in Liposomes |
| title_short | Formulation and Characterization of a Methacrylated Chitosan Topical Treatment with Dispersed Magnetite Nanoparticles Functionalized with Hydrophobic Drugs Encapsulated in Liposomes |
| title_sort | formulation and characterization of a methacrylated chitosan topical treatment with dispersed magnetite nanoparticles functionalized with hydrophobic drugs encapsulated in liposomes |
| topic | magnetoliposomes nanoparticles magnetite skin drug delivery ChiMa hydrogels |
| url | https://www.mdpi.com/2673-9976/20/1/27 |
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