Changes in microRNA Expression in the Cochlear Nucleus and Inferior Colliculus after Acute Noise-Induced Hearing Loss
Noise-induced hearing loss (NIHL) can lead to secondary changes that induce neural plasticity in the central auditory pathway. These changes include decreases in the number of synapses, the degeneration of auditory nerve fibers, and reorganization of the cochlear nucleus (CN) and inferior colliculus...
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MDPI AG
2020-11-01
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| Online Access: | https://www.mdpi.com/1422-0067/21/22/8792 |
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| author | Sohyeon Park Seung Hee Han Byeong-Gon Kim Myung-Whan Suh Jun Ho Lee Seung Ha Oh Moo Kyun Park |
| author_facet | Sohyeon Park Seung Hee Han Byeong-Gon Kim Myung-Whan Suh Jun Ho Lee Seung Ha Oh Moo Kyun Park |
| author_sort | Sohyeon Park |
| collection | DOAJ |
| description | Noise-induced hearing loss (NIHL) can lead to secondary changes that induce neural plasticity in the central auditory pathway. These changes include decreases in the number of synapses, the degeneration of auditory nerve fibers, and reorganization of the cochlear nucleus (CN) and inferior colliculus (IC) in the brain. This study investigated the role of microRNAs (miRNAs) in the neural plasticity of the central auditory pathway after acute NIHL. Male Sprague–Dawley rats were exposed to white band noise at 115 dB for 2 h, and the auditory brainstem response (ABR) and morphology of the organ of Corti were evaluated on days 1 and 3. Following noise exposure, the ABR threshold shift was significantly smaller in the day 3 group, while wave II amplitudes were significantly larger in the day 3 group compared to the day 1 group. The organ of Corti on the basal turn showed evidence of damage and the number of surviving outer hair cells was significantly lower in the basal and middle turn areas of the hearing loss groups relative to controls. Five and three candidate miRNAs for each CN and IC were selected based on microarray analysis and quantitative reverse transcription PCR (RT-qPCR). The data confirmed that even short-term acoustic stimulation can lead to changes in neuroplasticity. Further studies are needed to validate the role of these candidate miRNAs. Such miRNAs may be used in the early diagnosis and treatment of neural plasticity of the central auditory pathway after acute NIHL. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:42:25Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e01bfe9b2ef34ccf98bc5af7b99ed0152023-11-20T21:44:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012122879210.3390/ijms21228792Changes in microRNA Expression in the Cochlear Nucleus and Inferior Colliculus after Acute Noise-Induced Hearing LossSohyeon Park0Seung Hee Han1Byeong-Gon Kim2Myung-Whan Suh3Jun Ho Lee4Seung Ha Oh5Moo Kyun Park6Interdisciplinary Program in Neuroscience, Seoul National University College of Natural Sciences, Seoul 08826, KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul 03080, KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul 03080, KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul 03080, KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul 03080, KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul 03080, KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul 03080, KoreaNoise-induced hearing loss (NIHL) can lead to secondary changes that induce neural plasticity in the central auditory pathway. These changes include decreases in the number of synapses, the degeneration of auditory nerve fibers, and reorganization of the cochlear nucleus (CN) and inferior colliculus (IC) in the brain. This study investigated the role of microRNAs (miRNAs) in the neural plasticity of the central auditory pathway after acute NIHL. Male Sprague–Dawley rats were exposed to white band noise at 115 dB for 2 h, and the auditory brainstem response (ABR) and morphology of the organ of Corti were evaluated on days 1 and 3. Following noise exposure, the ABR threshold shift was significantly smaller in the day 3 group, while wave II amplitudes were significantly larger in the day 3 group compared to the day 1 group. The organ of Corti on the basal turn showed evidence of damage and the number of surviving outer hair cells was significantly lower in the basal and middle turn areas of the hearing loss groups relative to controls. Five and three candidate miRNAs for each CN and IC were selected based on microarray analysis and quantitative reverse transcription PCR (RT-qPCR). The data confirmed that even short-term acoustic stimulation can lead to changes in neuroplasticity. Further studies are needed to validate the role of these candidate miRNAs. Such miRNAs may be used in the early diagnosis and treatment of neural plasticity of the central auditory pathway after acute NIHL.https://www.mdpi.com/1422-0067/21/22/8792noise-induced hearing lossmicroRNAscochlear nucleusinferior colliculusneuroplasticity |
| spellingShingle | Sohyeon Park Seung Hee Han Byeong-Gon Kim Myung-Whan Suh Jun Ho Lee Seung Ha Oh Moo Kyun Park Changes in microRNA Expression in the Cochlear Nucleus and Inferior Colliculus after Acute Noise-Induced Hearing Loss International Journal of Molecular Sciences noise-induced hearing loss microRNAs cochlear nucleus inferior colliculus neuroplasticity |
| title | Changes in microRNA Expression in the Cochlear Nucleus and Inferior Colliculus after Acute Noise-Induced Hearing Loss |
| title_full | Changes in microRNA Expression in the Cochlear Nucleus and Inferior Colliculus after Acute Noise-Induced Hearing Loss |
| title_fullStr | Changes in microRNA Expression in the Cochlear Nucleus and Inferior Colliculus after Acute Noise-Induced Hearing Loss |
| title_full_unstemmed | Changes in microRNA Expression in the Cochlear Nucleus and Inferior Colliculus after Acute Noise-Induced Hearing Loss |
| title_short | Changes in microRNA Expression in the Cochlear Nucleus and Inferior Colliculus after Acute Noise-Induced Hearing Loss |
| title_sort | changes in microrna expression in the cochlear nucleus and inferior colliculus after acute noise induced hearing loss |
| topic | noise-induced hearing loss microRNAs cochlear nucleus inferior colliculus neuroplasticity |
| url | https://www.mdpi.com/1422-0067/21/22/8792 |
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