Different Profile of mRNA Expression in Sinoatrial Node from Streptozotocin-Induced Diabetic Rat.
BACKGROUND:Experiments in isolated perfused heart have shown that heart rate is lower and sinoatrial node (SAN) action potential duration is longer in streptozotocin (STZ)-induced diabetic rat compared to controls. In sino-atrial preparations the pacemaker cycle length and sino-atrial conduction tim...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4838258?pdf=render |
| _version_ | 1818947682838773760 |
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| author | Zannatul Ferdous Muhammad Anwar Qureshi Petrilla Jayaprakash Khatija Parekh Annie John Murat Oz Haider Raza Halina Dobrzynski Thomas Edward Adrian Frank Christopher Howarth |
| author_facet | Zannatul Ferdous Muhammad Anwar Qureshi Petrilla Jayaprakash Khatija Parekh Annie John Murat Oz Haider Raza Halina Dobrzynski Thomas Edward Adrian Frank Christopher Howarth |
| author_sort | Zannatul Ferdous |
| collection | DOAJ |
| description | BACKGROUND:Experiments in isolated perfused heart have shown that heart rate is lower and sinoatrial node (SAN) action potential duration is longer in streptozotocin (STZ)-induced diabetic rat compared to controls. In sino-atrial preparations the pacemaker cycle length and sino-atrial conduction time are prolonged in STZ heart. To further clarify the molecular basis of electrical disturbances in the diabetic heart the profile of mRNA encoding a wide variety of proteins associated with the generation and transmission of electrical activity has been evaluated in the SAN of STZ-induced diabetic rat heart. METHODOLOGY/PRINCIPAL FINDINGS:Heart rate was measured in isolated perfused heart with an extracellular suction electrode. Expression of mRNA encoding a variety of intercellular proteins, intracellular Ca2+-transport and regulatory proteins, cell membrane transport proteins and calcium, sodium and potassium channel proteins were measured in SAN and right atrial (RA) biopsies using real-time reverse transcription polymerase chain reaction techniques. Heart rate was lower in STZ (203±7 bpm) compared to control (239±11 bpm) rat. Among many differences in the profile of mRNA there are some worthy of particular emphasis. Expression of genes encoding some proteins were significantly downregulated in STZ-SAN: calcium channel, Cacng4 (7-fold); potassium channel, Kcnd2 whilst genes encoding some other proteins were significantly upregulated in STZ-SAN: gap junction, Gjc1; cell membrane transport, Slc8a1, Trpc1, Trpc6 (4-fold); intracellular Ca2+-transport, Ryr3; calcium channel Cacna1g, Cacna1h, Cacnb3; potassium channels, Kcnj5, Kcnk3 and natriuretic peptides, Nppa (5-fold) and Nppb (7-fold). CONCLUSIONS/SIGNIFICANCE:Collectively, this study has demonstrated differences in the profile of mRNA encoding a variety of proteins that are associated with the generation, conduction and regulation of electrical signals in the SAN of STZ-induced diabetic rat heart. Data from this study will provide a basis for a substantial range of future studies to investigate whether these changes in mRNA translate into changes in electrophysiological function. |
| first_indexed | 2024-12-20T08:34:47Z |
| format | Article |
| id | doaj.art-e01e4e5473cb40a3834c173c5dbe0c0d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T08:34:47Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e01e4e5473cb40a3834c173c5dbe0c0d2022-12-21T19:46:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01114e015393410.1371/journal.pone.0153934Different Profile of mRNA Expression in Sinoatrial Node from Streptozotocin-Induced Diabetic Rat.Zannatul FerdousMuhammad Anwar QureshiPetrilla JayaprakashKhatija ParekhAnnie JohnMurat OzHaider RazaHalina DobrzynskiThomas Edward AdrianFrank Christopher HowarthBACKGROUND:Experiments in isolated perfused heart have shown that heart rate is lower and sinoatrial node (SAN) action potential duration is longer in streptozotocin (STZ)-induced diabetic rat compared to controls. In sino-atrial preparations the pacemaker cycle length and sino-atrial conduction time are prolonged in STZ heart. To further clarify the molecular basis of electrical disturbances in the diabetic heart the profile of mRNA encoding a wide variety of proteins associated with the generation and transmission of electrical activity has been evaluated in the SAN of STZ-induced diabetic rat heart. METHODOLOGY/PRINCIPAL FINDINGS:Heart rate was measured in isolated perfused heart with an extracellular suction electrode. Expression of mRNA encoding a variety of intercellular proteins, intracellular Ca2+-transport and regulatory proteins, cell membrane transport proteins and calcium, sodium and potassium channel proteins were measured in SAN and right atrial (RA) biopsies using real-time reverse transcription polymerase chain reaction techniques. Heart rate was lower in STZ (203±7 bpm) compared to control (239±11 bpm) rat. Among many differences in the profile of mRNA there are some worthy of particular emphasis. Expression of genes encoding some proteins were significantly downregulated in STZ-SAN: calcium channel, Cacng4 (7-fold); potassium channel, Kcnd2 whilst genes encoding some other proteins were significantly upregulated in STZ-SAN: gap junction, Gjc1; cell membrane transport, Slc8a1, Trpc1, Trpc6 (4-fold); intracellular Ca2+-transport, Ryr3; calcium channel Cacna1g, Cacna1h, Cacnb3; potassium channels, Kcnj5, Kcnk3 and natriuretic peptides, Nppa (5-fold) and Nppb (7-fold). CONCLUSIONS/SIGNIFICANCE:Collectively, this study has demonstrated differences in the profile of mRNA encoding a variety of proteins that are associated with the generation, conduction and regulation of electrical signals in the SAN of STZ-induced diabetic rat heart. Data from this study will provide a basis for a substantial range of future studies to investigate whether these changes in mRNA translate into changes in electrophysiological function.http://europepmc.org/articles/PMC4838258?pdf=render |
| spellingShingle | Zannatul Ferdous Muhammad Anwar Qureshi Petrilla Jayaprakash Khatija Parekh Annie John Murat Oz Haider Raza Halina Dobrzynski Thomas Edward Adrian Frank Christopher Howarth Different Profile of mRNA Expression in Sinoatrial Node from Streptozotocin-Induced Diabetic Rat. PLoS ONE |
| title | Different Profile of mRNA Expression in Sinoatrial Node from Streptozotocin-Induced Diabetic Rat. |
| title_full | Different Profile of mRNA Expression in Sinoatrial Node from Streptozotocin-Induced Diabetic Rat. |
| title_fullStr | Different Profile of mRNA Expression in Sinoatrial Node from Streptozotocin-Induced Diabetic Rat. |
| title_full_unstemmed | Different Profile of mRNA Expression in Sinoatrial Node from Streptozotocin-Induced Diabetic Rat. |
| title_short | Different Profile of mRNA Expression in Sinoatrial Node from Streptozotocin-Induced Diabetic Rat. |
| title_sort | different profile of mrna expression in sinoatrial node from streptozotocin induced diabetic rat |
| url | http://europepmc.org/articles/PMC4838258?pdf=render |
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