T cells enhance gold nanoparticle delivery to tumors <it>in vivo</it>
<p>Abstract</p> <p>Gold nanoparticle-mediated photothermal therapy (PTT) has shown great potential for the treatment of cancer in mouse studies and is now being evaluated in clinical trials. For this therapy, gold nanoparticles (AuNPs) are injected intravenously and are allowed to...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2011-01-01
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| Series: | Nanoscale Research Letters |
| Online Access: | http://www.nanoscalereslett.com/content/6/1/283 |
| _version_ | 1797766614135865344 |
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| author | Bear Adham Foster Aaron Young Joseph Kennedy Laura Lewinski Nastassja Kim Jean Drezek Rebekah |
| author_facet | Bear Adham Foster Aaron Young Joseph Kennedy Laura Lewinski Nastassja Kim Jean Drezek Rebekah |
| author_sort | Bear Adham |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Gold nanoparticle-mediated photothermal therapy (PTT) has shown great potential for the treatment of cancer in mouse studies and is now being evaluated in clinical trials. For this therapy, gold nanoparticles (AuNPs) are injected intravenously and are allowed to accumulate within the tumor via the enhanced permeability and retention (EPR) effect. The tumor is then irradiated with a near infrared laser, whose energy is absorbed by the AuNPs and translated into heat. While reliance on the EPR effect for tumor targeting has proven adequate for vascularized tumors in small animal models, the efficiency and specificity of tumor delivery <it>in vivo</it>, particularly in tumors with poor blood supply, has proven challenging. In this study, we examine whether human T cells can be used as cellular delivery vehicles for AuNP transport into tumors. We first demonstrate that T cells can be efficiently loaded with 45 nm gold colloid nanoparticles without affecting viability or function (e.g. migration and cytokine production). Using a human tumor xenograft mouse model, we next demonstrate that AuNP-loaded T cells retain their capacity to migrate to tumor sites <it>in vivo</it>. In addition, the efficiency of AuNP delivery to tumors <it>in vivo </it>is increased by more than four-fold compared to injection of free PEGylated AuNPs and the use of the T cell delivery system also dramatically alters the overall nanoparticle biodistribution. Thus, the use of T cell chaperones for AuNP delivery could enhance the efficacy of nanoparticle-based therapies and imaging applications by increasing AuNP tumor accumulation.</p> |
| first_indexed | 2024-03-12T20:27:46Z |
| format | Article |
| id | doaj.art-e0208bdf839e412e891b3427a1338ad7 |
| institution | Directory Open Access Journal |
| issn | 1931-7573 1556-276X |
| language | English |
| last_indexed | 2024-03-12T20:27:46Z |
| publishDate | 2011-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-e0208bdf839e412e891b3427a1338ad72023-08-02T00:25:08ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2011-01-0161283T cells enhance gold nanoparticle delivery to tumors <it>in vivo</it>Bear AdhamFoster AaronYoung JosephKennedy LauraLewinski NastassjaKim JeanDrezek Rebekah<p>Abstract</p> <p>Gold nanoparticle-mediated photothermal therapy (PTT) has shown great potential for the treatment of cancer in mouse studies and is now being evaluated in clinical trials. For this therapy, gold nanoparticles (AuNPs) are injected intravenously and are allowed to accumulate within the tumor via the enhanced permeability and retention (EPR) effect. The tumor is then irradiated with a near infrared laser, whose energy is absorbed by the AuNPs and translated into heat. While reliance on the EPR effect for tumor targeting has proven adequate for vascularized tumors in small animal models, the efficiency and specificity of tumor delivery <it>in vivo</it>, particularly in tumors with poor blood supply, has proven challenging. In this study, we examine whether human T cells can be used as cellular delivery vehicles for AuNP transport into tumors. We first demonstrate that T cells can be efficiently loaded with 45 nm gold colloid nanoparticles without affecting viability or function (e.g. migration and cytokine production). Using a human tumor xenograft mouse model, we next demonstrate that AuNP-loaded T cells retain their capacity to migrate to tumor sites <it>in vivo</it>. In addition, the efficiency of AuNP delivery to tumors <it>in vivo </it>is increased by more than four-fold compared to injection of free PEGylated AuNPs and the use of the T cell delivery system also dramatically alters the overall nanoparticle biodistribution. Thus, the use of T cell chaperones for AuNP delivery could enhance the efficacy of nanoparticle-based therapies and imaging applications by increasing AuNP tumor accumulation.</p>http://www.nanoscalereslett.com/content/6/1/283 |
| spellingShingle | Bear Adham Foster Aaron Young Joseph Kennedy Laura Lewinski Nastassja Kim Jean Drezek Rebekah T cells enhance gold nanoparticle delivery to tumors <it>in vivo</it> Nanoscale Research Letters |
| title | T cells enhance gold nanoparticle delivery to tumors <it>in vivo</it> |
| title_full | T cells enhance gold nanoparticle delivery to tumors <it>in vivo</it> |
| title_fullStr | T cells enhance gold nanoparticle delivery to tumors <it>in vivo</it> |
| title_full_unstemmed | T cells enhance gold nanoparticle delivery to tumors <it>in vivo</it> |
| title_short | T cells enhance gold nanoparticle delivery to tumors <it>in vivo</it> |
| title_sort | t cells enhance gold nanoparticle delivery to tumors it in vivo it |
| url | http://www.nanoscalereslett.com/content/6/1/283 |
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