Therapeutic Effects of Oral Application of Menthol and Extracts from Tormentil (<i>Potentilla erecta</i>), Raspberry Leaves (<i>Rubus idaeus</i>), and Loosestrife (<i>Lythrum salicaria</i>) during Acute Murine Campylobacteriosis

Human food-borne infections with the enteropathogen <i>Campylobacter jejuni</i> are becoming increasingly prevalent worldwide. Since antibiotics are usually not indicated in campylobacteriosis, alternative treatment regimens are important. We here investigated potential disease-alleviati...

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Main Authors: Rasmus Bandick, Lia V. Busmann, Soraya Mousavi, Nizar W. Shayya, Jakub P. Piwowarski, Sebastian Granica, Matthias F. Melzig, Stefan Bereswill, Markus M. Heimesaat
Format: Article
Language:English
Published: MDPI AG 2023-10-01
Series:Pharmaceutics
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Online Access:https://www.mdpi.com/1999-4923/15/10/2410
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author Rasmus Bandick
Lia V. Busmann
Soraya Mousavi
Nizar W. Shayya
Jakub P. Piwowarski
Sebastian Granica
Matthias F. Melzig
Stefan Bereswill
Markus M. Heimesaat
author_facet Rasmus Bandick
Lia V. Busmann
Soraya Mousavi
Nizar W. Shayya
Jakub P. Piwowarski
Sebastian Granica
Matthias F. Melzig
Stefan Bereswill
Markus M. Heimesaat
author_sort Rasmus Bandick
collection DOAJ
description Human food-borne infections with the enteropathogen <i>Campylobacter jejuni</i> are becoming increasingly prevalent worldwide. Since antibiotics are usually not indicated in campylobacteriosis, alternative treatment regimens are important. We here investigated potential disease-alleviating effects of menthol and of extracts from tormentil, raspberry leaves, and loosestrife in acute murine campylobacteriosis. Therefore, <i>C. jejuni</i>-infected microbiota-depleted IL-10<sup>−/−</sup> mice were orally treated with the compounds alone or all in combination from day 2 until day 6 post-infection. Whereas neither treatment regimen affected gastrointestinal pathogen loads, the combination of compounds alleviated <i>C. jejuni</i>-induced diarrheal symptoms in diseased mice on day 6 post-infection. Furthermore, the therapeutic application of tormentil and menthol alone and the combination of the four compounds resulted in lower colonic T cell numbers in infected mice when compared to placebo counterparts. Notably, pro-inflammatory cytokines measured in mesenteric lymph nodes taken from <i>C. jejuni</i>-infected mice following tormentil, menthol, and combination treatment did not differ from basal concentrations. However, neither treatment regimen could dampen extra-intestinal immune responses, including systemic pro-inflammatory cytokine secretion on day 6 post-infection. In conclusion, the combination of menthol and of extracts from tormentil, raspberry leaves, and loosestrife constitutes an antibiotic-independent approach to alleviate campylobacteriosis symptoms.
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spelling doaj.art-e021b0da0553410bb77fc1ca5e6b75a22023-11-19T17:44:07ZengMDPI AGPharmaceutics1999-49232023-10-011510241010.3390/pharmaceutics15102410Therapeutic Effects of Oral Application of Menthol and Extracts from Tormentil (<i>Potentilla erecta</i>), Raspberry Leaves (<i>Rubus idaeus</i>), and Loosestrife (<i>Lythrum salicaria</i>) during Acute Murine CampylobacteriosisRasmus Bandick0Lia V. Busmann1Soraya Mousavi2Nizar W. Shayya3Jakub P. Piwowarski4Sebastian Granica5Matthias F. Melzig6Stefan Bereswill7Markus M. Heimesaat8Gastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, D-12203 Berlin, GermanyGastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, D-12203 Berlin, GermanyGastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, D-12203 Berlin, GermanyGastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, D-12203 Berlin, GermanyMicrobiota Lab, Department of Pharmaceutical Biology, Medical University of Warsaw, 02-097 Warsaw, PolandMicrobiota Lab, Department of Pharmaceutical Biology, Medical University of Warsaw, 02-097 Warsaw, PolandInstitute of Pharmacy, Freie Universität Berlin, D-14195 Berlin, GermanyGastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, D-12203 Berlin, GermanyGastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, D-12203 Berlin, GermanyHuman food-borne infections with the enteropathogen <i>Campylobacter jejuni</i> are becoming increasingly prevalent worldwide. Since antibiotics are usually not indicated in campylobacteriosis, alternative treatment regimens are important. We here investigated potential disease-alleviating effects of menthol and of extracts from tormentil, raspberry leaves, and loosestrife in acute murine campylobacteriosis. Therefore, <i>C. jejuni</i>-infected microbiota-depleted IL-10<sup>−/−</sup> mice were orally treated with the compounds alone or all in combination from day 2 until day 6 post-infection. Whereas neither treatment regimen affected gastrointestinal pathogen loads, the combination of compounds alleviated <i>C. jejuni</i>-induced diarrheal symptoms in diseased mice on day 6 post-infection. Furthermore, the therapeutic application of tormentil and menthol alone and the combination of the four compounds resulted in lower colonic T cell numbers in infected mice when compared to placebo counterparts. Notably, pro-inflammatory cytokines measured in mesenteric lymph nodes taken from <i>C. jejuni</i>-infected mice following tormentil, menthol, and combination treatment did not differ from basal concentrations. However, neither treatment regimen could dampen extra-intestinal immune responses, including systemic pro-inflammatory cytokine secretion on day 6 post-infection. In conclusion, the combination of menthol and of extracts from tormentil, raspberry leaves, and loosestrife constitutes an antibiotic-independent approach to alleviate campylobacteriosis symptoms.https://www.mdpi.com/1999-4923/15/10/2410tormentil (<i>Potentilla erecta</i>)raspberry leaves (<i>Rubus idaeus</i>)loosestrife (<i>Lythrum salicaria</i>)mentholplant-derived compounds<i>Campylobacter jejuni</i>
spellingShingle Rasmus Bandick
Lia V. Busmann
Soraya Mousavi
Nizar W. Shayya
Jakub P. Piwowarski
Sebastian Granica
Matthias F. Melzig
Stefan Bereswill
Markus M. Heimesaat
Therapeutic Effects of Oral Application of Menthol and Extracts from Tormentil (<i>Potentilla erecta</i>), Raspberry Leaves (<i>Rubus idaeus</i>), and Loosestrife (<i>Lythrum salicaria</i>) during Acute Murine Campylobacteriosis
Pharmaceutics
tormentil (<i>Potentilla erecta</i>)
raspberry leaves (<i>Rubus idaeus</i>)
loosestrife (<i>Lythrum salicaria</i>)
menthol
plant-derived compounds
<i>Campylobacter jejuni</i>
title Therapeutic Effects of Oral Application of Menthol and Extracts from Tormentil (<i>Potentilla erecta</i>), Raspberry Leaves (<i>Rubus idaeus</i>), and Loosestrife (<i>Lythrum salicaria</i>) during Acute Murine Campylobacteriosis
title_full Therapeutic Effects of Oral Application of Menthol and Extracts from Tormentil (<i>Potentilla erecta</i>), Raspberry Leaves (<i>Rubus idaeus</i>), and Loosestrife (<i>Lythrum salicaria</i>) during Acute Murine Campylobacteriosis
title_fullStr Therapeutic Effects of Oral Application of Menthol and Extracts from Tormentil (<i>Potentilla erecta</i>), Raspberry Leaves (<i>Rubus idaeus</i>), and Loosestrife (<i>Lythrum salicaria</i>) during Acute Murine Campylobacteriosis
title_full_unstemmed Therapeutic Effects of Oral Application of Menthol and Extracts from Tormentil (<i>Potentilla erecta</i>), Raspberry Leaves (<i>Rubus idaeus</i>), and Loosestrife (<i>Lythrum salicaria</i>) during Acute Murine Campylobacteriosis
title_short Therapeutic Effects of Oral Application of Menthol and Extracts from Tormentil (<i>Potentilla erecta</i>), Raspberry Leaves (<i>Rubus idaeus</i>), and Loosestrife (<i>Lythrum salicaria</i>) during Acute Murine Campylobacteriosis
title_sort therapeutic effects of oral application of menthol and extracts from tormentil i potentilla erecta i raspberry leaves i rubus idaeus i and loosestrife i lythrum salicaria i during acute murine campylobacteriosis
topic tormentil (<i>Potentilla erecta</i>)
raspberry leaves (<i>Rubus idaeus</i>)
loosestrife (<i>Lythrum salicaria</i>)
menthol
plant-derived compounds
<i>Campylobacter jejuni</i>
url https://www.mdpi.com/1999-4923/15/10/2410
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