Effectiveness of a Simultaneous rHVT-F(ND) and rHVT-H5(AI) Vaccination of Day-Old Chickens and the Influence of NDV- and AIV-Specific MDA on Immune Response and Conferred Protection
The recombinant herpesvirus of turkey (rHVT) vaccines targeting Newcastle disease (ND) and H5Nx avian influenza (AI) have been demonstrated efficient in chickens when used individually at day-old. Given the practical field constraints associated with administering two vaccines separately and in the...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-09-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/8/3/536 |
| _version_ | 1797553547878858752 |
|---|---|
| author | Fabienne Rauw Eva Ngabirano Yannick Gardin Vilmos Palya Bénédicte Lambrecht |
| author_facet | Fabienne Rauw Eva Ngabirano Yannick Gardin Vilmos Palya Bénédicte Lambrecht |
| author_sort | Fabienne Rauw |
| collection | DOAJ |
| description | The recombinant herpesvirus of turkey (rHVT) vaccines targeting Newcastle disease (ND) and H5Nx avian influenza (AI) have been demonstrated efficient in chickens when used individually at day-old. Given the practical field constraints associated with administering two vaccines separately and in the absence of a currently available bivalent rHVT vector vaccine expressing both F(ND) and H5(AI) antigens, the aim of this study was to investigate whether interference occurs between the two vaccines when simultaneously administered in a single shot. The studies have been designed to determine (i) the ND and AI-specific protection and antibody response conferred by these vaccines inoculated alone or in combination at day-old, (ii) the influence of maternally-derived antibodies (MDA), and (iii) the potential interference between the two vaccine. Our results demonstrate that their combined administration is efficient to protect chickens against clinical signs of velogenic Newcastle disease virus (vNDV) and H5-highly pathogenic avian influenza virus (HPAIV) infections. Viral shedding following co-vaccination is also markedly reduced, while slightly lower NDV- and AIV-specific antibody responses are observed. NDV- and AIV-specific MDA show negative effects on the onset of the specific antibody responses. However, if AIV-specific MDA reduce the protection against H5-HPAIV induced by rHVT-H5(AI) vaccine, it was not observed for ND. |
| first_indexed | 2024-03-10T16:17:07Z |
| format | Article |
| id | doaj.art-e03205427df14f85a5490fe587e507f7 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T16:17:07Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-e03205427df14f85a5490fe587e507f72023-11-20T13:58:48ZengMDPI AGVaccines2076-393X2020-09-018353610.3390/vaccines8030536Effectiveness of a Simultaneous rHVT-F(ND) and rHVT-H5(AI) Vaccination of Day-Old Chickens and the Influence of NDV- and AIV-Specific MDA on Immune Response and Conferred ProtectionFabienne Rauw0Eva Ngabirano1Yannick Gardin2Vilmos Palya3Bénédicte Lambrecht4Sciensano, Avian Virology and Immunology Service, Groeselenberg 99, 1180 Brussels, BelgiumSciensano, Avian Virology and Immunology Service, Groeselenberg 99, 1180 Brussels, BelgiumCEVA Santé Animale, Avenue de la Ballastière 10, 33 500 Libourne, FranceCEVA Phylaxia, Szállás utca 5, 1107 Budapest, HungarySciensano, Avian Virology and Immunology Service, Groeselenberg 99, 1180 Brussels, BelgiumThe recombinant herpesvirus of turkey (rHVT) vaccines targeting Newcastle disease (ND) and H5Nx avian influenza (AI) have been demonstrated efficient in chickens when used individually at day-old. Given the practical field constraints associated with administering two vaccines separately and in the absence of a currently available bivalent rHVT vector vaccine expressing both F(ND) and H5(AI) antigens, the aim of this study was to investigate whether interference occurs between the two vaccines when simultaneously administered in a single shot. The studies have been designed to determine (i) the ND and AI-specific protection and antibody response conferred by these vaccines inoculated alone or in combination at day-old, (ii) the influence of maternally-derived antibodies (MDA), and (iii) the potential interference between the two vaccine. Our results demonstrate that their combined administration is efficient to protect chickens against clinical signs of velogenic Newcastle disease virus (vNDV) and H5-highly pathogenic avian influenza virus (HPAIV) infections. Viral shedding following co-vaccination is also markedly reduced, while slightly lower NDV- and AIV-specific antibody responses are observed. NDV- and AIV-specific MDA show negative effects on the onset of the specific antibody responses. However, if AIV-specific MDA reduce the protection against H5-HPAIV induced by rHVT-H5(AI) vaccine, it was not observed for ND.https://www.mdpi.com/2076-393X/8/3/536chickenNewcastle diseaseavian influenzarecombinant vaccineturkey herpesvirusprotection |
| spellingShingle | Fabienne Rauw Eva Ngabirano Yannick Gardin Vilmos Palya Bénédicte Lambrecht Effectiveness of a Simultaneous rHVT-F(ND) and rHVT-H5(AI) Vaccination of Day-Old Chickens and the Influence of NDV- and AIV-Specific MDA on Immune Response and Conferred Protection Vaccines chicken Newcastle disease avian influenza recombinant vaccine turkey herpesvirus protection |
| title | Effectiveness of a Simultaneous rHVT-F(ND) and rHVT-H5(AI) Vaccination of Day-Old Chickens and the Influence of NDV- and AIV-Specific MDA on Immune Response and Conferred Protection |
| title_full | Effectiveness of a Simultaneous rHVT-F(ND) and rHVT-H5(AI) Vaccination of Day-Old Chickens and the Influence of NDV- and AIV-Specific MDA on Immune Response and Conferred Protection |
| title_fullStr | Effectiveness of a Simultaneous rHVT-F(ND) and rHVT-H5(AI) Vaccination of Day-Old Chickens and the Influence of NDV- and AIV-Specific MDA on Immune Response and Conferred Protection |
| title_full_unstemmed | Effectiveness of a Simultaneous rHVT-F(ND) and rHVT-H5(AI) Vaccination of Day-Old Chickens and the Influence of NDV- and AIV-Specific MDA on Immune Response and Conferred Protection |
| title_short | Effectiveness of a Simultaneous rHVT-F(ND) and rHVT-H5(AI) Vaccination of Day-Old Chickens and the Influence of NDV- and AIV-Specific MDA on Immune Response and Conferred Protection |
| title_sort | effectiveness of a simultaneous rhvt f nd and rhvt h5 ai vaccination of day old chickens and the influence of ndv and aiv specific mda on immune response and conferred protection |
| topic | chicken Newcastle disease avian influenza recombinant vaccine turkey herpesvirus protection |
| url | https://www.mdpi.com/2076-393X/8/3/536 |
| work_keys_str_mv | AT fabiennerauw effectivenessofasimultaneousrhvtfndandrhvth5aivaccinationofdayoldchickensandtheinfluenceofndvandaivspecificmdaonimmuneresponseandconferredprotection AT evangabirano effectivenessofasimultaneousrhvtfndandrhvth5aivaccinationofdayoldchickensandtheinfluenceofndvandaivspecificmdaonimmuneresponseandconferredprotection AT yannickgardin effectivenessofasimultaneousrhvtfndandrhvth5aivaccinationofdayoldchickensandtheinfluenceofndvandaivspecificmdaonimmuneresponseandconferredprotection AT vilmospalya effectivenessofasimultaneousrhvtfndandrhvth5aivaccinationofdayoldchickensandtheinfluenceofndvandaivspecificmdaonimmuneresponseandconferredprotection AT benedictelambrecht effectivenessofasimultaneousrhvtfndandrhvth5aivaccinationofdayoldchickensandtheinfluenceofndvandaivspecificmdaonimmuneresponseandconferredprotection |