Search for Associations of <i>FSHR</i>, <i>INHA</i>, <i>INHAB</i>, <i>PRL</i>, <i>TNP2</i> and <i>SPEF2</i> Genes Polymorphisms with Semen Quality in Russian Holstein Bulls (Pilot Study)
The aim of the study was to search for new mutations in the previously studied gene loci of follicle-stimulating hormone receptor (<i>FSHR</i>), inhibin α (<i>INHA</i>), inhibin β A (<i>INHAB</i>), prolactin (<i>PRL</i>), transition protein 2 (<i>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/10/2882 |
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| author | Elena Nikitkina Anna Krutikova Artem Musidray Kirill Plemyashov |
| author_facet | Elena Nikitkina Anna Krutikova Artem Musidray Kirill Plemyashov |
| author_sort | Elena Nikitkina |
| collection | DOAJ |
| description | The aim of the study was to search for new mutations in the previously studied gene loci of follicle-stimulating hormone receptor (<i>FSHR</i>), inhibin α (<i>INHA</i>), inhibin β A (<i>INHAB</i>), prolactin (<i>PRL</i>), transition protein 2 (<i>TNP2</i>), and sperm flagella 2 (<i>SPEF2</i>) by sequencing, as well as the search for associations of previously identified mutations at these loci with fresh semen quality in Russian Holstein bulls. Phenotypic data from 189 bulls was collected. Data was analyzed for most bulls for three years of semen collection. The maximum value of each semen quality indicator (doublet ejaculate volume, sperm concentration, progressive motility and total number of spermatozoa) were selected. SNPs were identified in the <i>FSHR</i>, <i>INHA</i>, <i>INHAB</i>, <i>TNP2</i>, <i>SPEF2</i> genes. The <i>PRL</i> gene did not have polymorphism. Significant (<i>p</i> < 0.05) associations of polymorphisms in the <i>FSHR</i> gene with double ejaculate volume, concentration and total number of spermatozoa were identified. Polymorphism in the <i>INHA</i> gene was significantly associated (<i>p</i> < 0.05) with sperm concentration. Polymorphism in the <i>INHAB</i> gene was significantly associated (<i>p</i> < 0.05) with doublet ejaculate volume and total number of spermatozoa. Polymorphisms in the <i>TNP2</i> and <i>SPEF2</i> genes did not have significant associations with semen quality. The SNPs studied in our pilot work may be considered as candidate genetic markers in the selection of bulls. |
| first_indexed | 2024-03-10T06:47:44Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T06:47:44Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-e0361b77b07b4014895a424929b8ca412023-11-22T17:10:39ZengMDPI AGAnimals2076-26152021-10-011110288210.3390/ani11102882Search for Associations of <i>FSHR</i>, <i>INHA</i>, <i>INHAB</i>, <i>PRL</i>, <i>TNP2</i> and <i>SPEF2</i> Genes Polymorphisms with Semen Quality in Russian Holstein Bulls (Pilot Study)Elena Nikitkina0Anna Krutikova1Artem Musidray2Kirill Plemyashov3Russian Research Institute of Farm Animal Genetics and Breeding—Branch of the L.K. Ernst Federal Research Center for Animal Husbandry, 55A, Moskovskoye sh., 196625 Saint-Petersburg, RussiaRussian Research Institute of Farm Animal Genetics and Breeding—Branch of the L.K. Ernst Federal Research Center for Animal Husbandry, 55A, Moskovskoye sh., 196625 Saint-Petersburg, RussiaRussian Research Institute of Farm Animal Genetics and Breeding—Branch of the L.K. Ernst Federal Research Center for Animal Husbandry, 55A, Moskovskoye sh., 196625 Saint-Petersburg, RussiaRussian Research Institute of Farm Animal Genetics and Breeding—Branch of the L.K. Ernst Federal Research Center for Animal Husbandry, 55A, Moskovskoye sh., 196625 Saint-Petersburg, RussiaThe aim of the study was to search for new mutations in the previously studied gene loci of follicle-stimulating hormone receptor (<i>FSHR</i>), inhibin α (<i>INHA</i>), inhibin β A (<i>INHAB</i>), prolactin (<i>PRL</i>), transition protein 2 (<i>TNP2</i>), and sperm flagella 2 (<i>SPEF2</i>) by sequencing, as well as the search for associations of previously identified mutations at these loci with fresh semen quality in Russian Holstein bulls. Phenotypic data from 189 bulls was collected. Data was analyzed for most bulls for three years of semen collection. The maximum value of each semen quality indicator (doublet ejaculate volume, sperm concentration, progressive motility and total number of spermatozoa) were selected. SNPs were identified in the <i>FSHR</i>, <i>INHA</i>, <i>INHAB</i>, <i>TNP2</i>, <i>SPEF2</i> genes. The <i>PRL</i> gene did not have polymorphism. Significant (<i>p</i> < 0.05) associations of polymorphisms in the <i>FSHR</i> gene with double ejaculate volume, concentration and total number of spermatozoa were identified. Polymorphism in the <i>INHA</i> gene was significantly associated (<i>p</i> < 0.05) with sperm concentration. Polymorphism in the <i>INHAB</i> gene was significantly associated (<i>p</i> < 0.05) with doublet ejaculate volume and total number of spermatozoa. Polymorphisms in the <i>TNP2</i> and <i>SPEF2</i> genes did not have significant associations with semen quality. The SNPs studied in our pilot work may be considered as candidate genetic markers in the selection of bulls.https://www.mdpi.com/2076-2615/11/10/2882sperm qualitybullscandidate genespolymorphism |
| spellingShingle | Elena Nikitkina Anna Krutikova Artem Musidray Kirill Plemyashov Search for Associations of <i>FSHR</i>, <i>INHA</i>, <i>INHAB</i>, <i>PRL</i>, <i>TNP2</i> and <i>SPEF2</i> Genes Polymorphisms with Semen Quality in Russian Holstein Bulls (Pilot Study) Animals sperm quality bulls candidate genes polymorphism |
| title | Search for Associations of <i>FSHR</i>, <i>INHA</i>, <i>INHAB</i>, <i>PRL</i>, <i>TNP2</i> and <i>SPEF2</i> Genes Polymorphisms with Semen Quality in Russian Holstein Bulls (Pilot Study) |
| title_full | Search for Associations of <i>FSHR</i>, <i>INHA</i>, <i>INHAB</i>, <i>PRL</i>, <i>TNP2</i> and <i>SPEF2</i> Genes Polymorphisms with Semen Quality in Russian Holstein Bulls (Pilot Study) |
| title_fullStr | Search for Associations of <i>FSHR</i>, <i>INHA</i>, <i>INHAB</i>, <i>PRL</i>, <i>TNP2</i> and <i>SPEF2</i> Genes Polymorphisms with Semen Quality in Russian Holstein Bulls (Pilot Study) |
| title_full_unstemmed | Search for Associations of <i>FSHR</i>, <i>INHA</i>, <i>INHAB</i>, <i>PRL</i>, <i>TNP2</i> and <i>SPEF2</i> Genes Polymorphisms with Semen Quality in Russian Holstein Bulls (Pilot Study) |
| title_short | Search for Associations of <i>FSHR</i>, <i>INHA</i>, <i>INHAB</i>, <i>PRL</i>, <i>TNP2</i> and <i>SPEF2</i> Genes Polymorphisms with Semen Quality in Russian Holstein Bulls (Pilot Study) |
| title_sort | search for associations of i fshr i i inha i i inhab i i prl i i tnp2 i and i spef2 i genes polymorphisms with semen quality in russian holstein bulls pilot study |
| topic | sperm quality bulls candidate genes polymorphism |
| url | https://www.mdpi.com/2076-2615/11/10/2882 |
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