Whole-genome sequencing reveals highly specific gene targeting by in vitro assembled Cas9-ribonucleoprotein complexes in Aspergillus fumigatus

Whole-genome sequencing reveals highly specific gene targeting by in vitro assembled Cas9-ribonucleoprotein complexes in Aspergillus fumigatus

Abstract Background CRISPR/Cas9-based genome editing is quickly becoming a powerful tool within the field of fungal genetics. Adaptation of CRISPR/Cas9 systems are allowing for rapid and highly efficient gene targeting within fungi. We recently reported the adaptation of a simple CRISPR/Cas9 system...

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Bibliographic Details
Main Authors: Qusai Al Abdallah, Ana Camila Oliveira Souza, Adela Martin-Vicente, Wenbo Ge, Jarrod R. Fortwendel
Format: Article
Language:English
Published: BMC 2018-06-01
Series:Fungal Biology and Biotechnology
Subjects:
Aspergillus fumigatus
CRISPR/Cas9
Genome editing
Off-target mutation
Online Access:http://link.springer.com/article/10.1186/s40694-018-0057-2

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