Unmasking the efficacy of Skipjack Tuna (Katsuwonus Pelamis) dark muscle hydrolyzate in lipid regulation: a promising component for functional food
ABSTRACTTo effectively utilize the by-products of skipjack tuna (Katsuwonus Pelamis) for the preparation of peptides with lipid-lowering activity, alkaline proteinase was selected from six commercial proteases to hydrolyze dark muscle. An L16 (54) orthogonal design was employed to optimize the hydro...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | International Journal of Food Properties |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/10942912.2023.2267788 |
| _version_ | 1797386005121073152 |
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| author | Fangfang Huang Qingfei Dai Kewei Zheng Qingbao Ma Yu Liu Wei Jiang Xiaojun Yan |
| author_facet | Fangfang Huang Qingfei Dai Kewei Zheng Qingbao Ma Yu Liu Wei Jiang Xiaojun Yan |
| author_sort | Fangfang Huang |
| collection | DOAJ |
| description | ABSTRACTTo effectively utilize the by-products of skipjack tuna (Katsuwonus Pelamis) for the preparation of peptides with lipid-lowering activity, alkaline proteinase was selected from six commercial proteases to hydrolyze dark muscle. An L16 (54) orthogonal design was employed to optimize the hydrolysis conditions, resulting in the production of a high-peptide-content hydrolyzate named KPHs-AL. This hydrolyzate exhibited the ability to significantly reduce TG content. KPHs-AL also showed higher persuasive pancreatic lipase (PL) and cholesterol esterase (CEase) inhibitory potential than various molecular weight (Mw) fractions and other enzymatic hydrolyzates, with IC50 values of 5.14 and 8.51 mg/mL, respectively. Furthermore, KPHs-AL exerted a combined effect of regulating lipid accumulation in FAA-induced steatosis HepG2 cells, leading to decreased total cholesterol (TC) and total triglyceride (TG). Based on the high binding likelihood and interaction with specific binding sites predicted by Pepsite2, 38 peptide sequences were identified as potential PL and CEase inhibitors. Hence, KPHs-AL holds promise as a beneficial component of functional food and exhibits the potential for anti-obesity effects through the alleviation of lipid accumulation |
| first_indexed | 2024-03-08T22:03:40Z |
| format | Article |
| id | doaj.art-e036cf70c053486692796b4a16b42db8 |
| institution | Directory Open Access Journal |
| issn | 1094-2912 1532-2386 |
| language | English |
| last_indexed | 2024-03-08T22:03:40Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Food Properties |
| spelling | doaj.art-e036cf70c053486692796b4a16b42db82023-12-19T10:46:33ZengTaylor & Francis GroupInternational Journal of Food Properties1094-29121532-23862023-12-012623014302910.1080/10942912.2023.2267788Unmasking the efficacy of Skipjack Tuna (Katsuwonus Pelamis) dark muscle hydrolyzate in lipid regulation: a promising component for functional foodFangfang Huang0Qingfei Dai1Kewei Zheng2Qingbao Ma3Yu Liu4Wei Jiang5Xiaojun Yan6Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo, ChinaInstitute of Innovation and Application, Zhejiang Ocean University, Zhoushan, ChinaInstitute of Innovation and Application, Zhejiang Ocean University, Zhoushan, ChinaInstitute of Innovation and Application, Zhejiang Ocean University, Zhoushan, ChinaKey Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo, ChinaKey Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo, ChinaKey Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo, ChinaABSTRACTTo effectively utilize the by-products of skipjack tuna (Katsuwonus Pelamis) for the preparation of peptides with lipid-lowering activity, alkaline proteinase was selected from six commercial proteases to hydrolyze dark muscle. An L16 (54) orthogonal design was employed to optimize the hydrolysis conditions, resulting in the production of a high-peptide-content hydrolyzate named KPHs-AL. This hydrolyzate exhibited the ability to significantly reduce TG content. KPHs-AL also showed higher persuasive pancreatic lipase (PL) and cholesterol esterase (CEase) inhibitory potential than various molecular weight (Mw) fractions and other enzymatic hydrolyzates, with IC50 values of 5.14 and 8.51 mg/mL, respectively. Furthermore, KPHs-AL exerted a combined effect of regulating lipid accumulation in FAA-induced steatosis HepG2 cells, leading to decreased total cholesterol (TC) and total triglyceride (TG). Based on the high binding likelihood and interaction with specific binding sites predicted by Pepsite2, 38 peptide sequences were identified as potential PL and CEase inhibitors. Hence, KPHs-AL holds promise as a beneficial component of functional food and exhibits the potential for anti-obesity effects through the alleviation of lipid accumulationhttps://www.tandfonline.com/doi/10.1080/10942912.2023.2267788Skipjack tuna (Katsuwonus Pelamis)dark musclepeptideslipid accumulationPancreatic lipasecholesterol esterase |
| spellingShingle | Fangfang Huang Qingfei Dai Kewei Zheng Qingbao Ma Yu Liu Wei Jiang Xiaojun Yan Unmasking the efficacy of Skipjack Tuna (Katsuwonus Pelamis) dark muscle hydrolyzate in lipid regulation: a promising component for functional food International Journal of Food Properties Skipjack tuna (Katsuwonus Pelamis) dark muscle peptides lipid accumulation Pancreatic lipase cholesterol esterase |
| title | Unmasking the efficacy of Skipjack Tuna (Katsuwonus Pelamis) dark muscle hydrolyzate in lipid regulation: a promising component for functional food |
| title_full | Unmasking the efficacy of Skipjack Tuna (Katsuwonus Pelamis) dark muscle hydrolyzate in lipid regulation: a promising component for functional food |
| title_fullStr | Unmasking the efficacy of Skipjack Tuna (Katsuwonus Pelamis) dark muscle hydrolyzate in lipid regulation: a promising component for functional food |
| title_full_unstemmed | Unmasking the efficacy of Skipjack Tuna (Katsuwonus Pelamis) dark muscle hydrolyzate in lipid regulation: a promising component for functional food |
| title_short | Unmasking the efficacy of Skipjack Tuna (Katsuwonus Pelamis) dark muscle hydrolyzate in lipid regulation: a promising component for functional food |
| title_sort | unmasking the efficacy of skipjack tuna katsuwonus pelamis dark muscle hydrolyzate in lipid regulation a promising component for functional food |
| topic | Skipjack tuna (Katsuwonus Pelamis) dark muscle peptides lipid accumulation Pancreatic lipase cholesterol esterase |
| url | https://www.tandfonline.com/doi/10.1080/10942912.2023.2267788 |
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