Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD)
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterized patient with autism spectrum disorder (ASD). The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The pluripotency of transgene-free iPSCs was verified b...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017-05-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187350611730051X |
| _version_ | 1819267140423778304 |
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| author | Eszter Varga Csilla Nemes István Bock Zsuzsanna Táncos Sára Berzsenyi György Lévay Viktor Román Julianna Kobolák András Dinnyés |
| author_facet | Eszter Varga Csilla Nemes István Bock Zsuzsanna Táncos Sára Berzsenyi György Lévay Viktor Román Julianna Kobolák András Dinnyés |
| author_sort | Eszter Varga |
| collection | DOAJ |
| description | Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterized patient with autism spectrum disorder (ASD). The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The pluripotency of transgene-free iPSCs was verified by immunocytochemistry for pluripotency markers and by spontaneous in vitro differentiation towards the 3 germ layers. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to study the pathomechanism of ASD, also for drug testing, early biomarker discovery and gene therapy studies. |
| first_indexed | 2024-12-23T21:12:26Z |
| format | Article |
| id | doaj.art-e038f76d70a14bd0b646c9f7c2bd321a |
| institution | Directory Open Access Journal |
| issn | 1873-5061 1876-7753 |
| language | English |
| last_indexed | 2024-12-23T21:12:26Z |
| publishDate | 2017-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-e038f76d70a14bd0b646c9f7c2bd321a2022-12-21T17:31:02ZengElsevierStem Cell Research1873-50611876-77532017-05-0121C192210.1016/j.scr.2017.03.013Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD)Eszter Varga0Csilla Nemes1István Bock2Zsuzsanna Táncos3Sára Berzsenyi4György Lévay5Viktor Román6Julianna Kobolák7András Dinnyés8BioTalentum Ltd., Gödöllő, HungaryBioTalentum Ltd., Gödöllő, HungaryBioTalentum Ltd., Gödöllő, HungaryBioTalentum Ltd., Gödöllő, HungaryPharmacology and Drug Safety Research, Gedeon Richter Plc., Budapest, HungaryPharmacology and Drug Safety Research, Gedeon Richter Plc., Budapest, HungaryPharmacology and Drug Safety Research, Gedeon Richter Plc., Budapest, HungaryBioTalentum Ltd., Gödöllő, HungaryBioTalentum Ltd., Gödöllő, HungaryPeripheral blood mononuclear cells (PBMCs) were collected from a clinically characterized patient with autism spectrum disorder (ASD). The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The pluripotency of transgene-free iPSCs was verified by immunocytochemistry for pluripotency markers and by spontaneous in vitro differentiation towards the 3 germ layers. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to study the pathomechanism of ASD, also for drug testing, early biomarker discovery and gene therapy studies.http://www.sciencedirect.com/science/article/pii/S187350611730051X |
| spellingShingle | Eszter Varga Csilla Nemes István Bock Zsuzsanna Táncos Sára Berzsenyi György Lévay Viktor Román Julianna Kobolák András Dinnyés Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD) Stem Cell Research |
| title | Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD) |
| title_full | Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD) |
| title_fullStr | Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD) |
| title_full_unstemmed | Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD) |
| title_short | Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD) |
| title_sort | establishment of an induced pluripotent stem cell ipsc line from a 9 year old male with autism spectrum disorder asd |
| url | http://www.sciencedirect.com/science/article/pii/S187350611730051X |
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