In Vitro Phytochemical Screening, Cytotoxicity Studies of <i>Curcuma longa</i> Extracts with Isolation and Characterisation of Their Isolated Compounds
The <i>Curcuma longa</i> plant is endowed with multiple traditional and therapeutic utilities and is here explored for its phytochemical constituents and cytotoxic potential. Turmeric rhizomes were extracted from three different solvents and screened for the presence of different phytoch...
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2021-12-01
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| author | Madhuri Grover Tapan Behl Aayush Sehgal Sukhbir Singh Neelam Sharma Tarun Virmani Mahesh Rachamalla Abdullah Farasani Sridevi Chigurupati Amal M. Alsubayiel Shatha Ghazi Felemban Mohit Sanduja Simona Bungau |
| author_facet | Madhuri Grover Tapan Behl Aayush Sehgal Sukhbir Singh Neelam Sharma Tarun Virmani Mahesh Rachamalla Abdullah Farasani Sridevi Chigurupati Amal M. Alsubayiel Shatha Ghazi Felemban Mohit Sanduja Simona Bungau |
| author_sort | Madhuri Grover |
| collection | DOAJ |
| description | The <i>Curcuma longa</i> plant is endowed with multiple traditional and therapeutic utilities and is here explored for its phytochemical constituents and cytotoxic potential. Turmeric rhizomes were extracted from three different solvents and screened for the presence of different phytochemical constituents, observation of which indicated that the polar solvents favoured extraction of greater versatile phytochemical constituents. These extracts were investigated for their cytotoxic potential by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay on three different of cell lines including SCC-29B (oral cancer cell line), DU-145 (prostate cancer cell line) and the Vero cell line (healthy cell line/non-cancerous cell line). This assay was performed by taking three extracts from isolated curcuminoids and a pure bioactive compound bisdemethoxycurcumin (BD). Bisdemethoxycurcumin was isolated from curcuminoids and purified by column and thin-layer chromatography, and its structural characterisation was performed with different spectroscopic techniques such as FTIR, NMR (<sup>1</sup>H Proton and <sup>13</sup>C Carbon-NMR) and LC-MS. Amongst the extracts, the ethanolic extracts exhibited stronger cytotoxic potential against the oral cancer cell line (SCC-29B) with an IC<sub>50</sub>value of 11.27 μg/mL, and that this was too low of a cytotoxicity against the Vero cell line. Although, curcuminoids have also shown a comparable cytotoxic potential against SCC-29B (IC<sub>50</sub> value 16.79 μg/mL), it was not as potent against the ethanolic extract, and it was even found to be cytotoxic against healthy cell lines at a very low dose. While considering the isolated compound, bisdemethoxycurcumin, it also possessed a cytotoxic potential against the prostate cancer cell line (DU-145) (IC<sub>50</sub> value of 93.28 μg/mL), but was quite safe for the healthy cell line in comparison to doxorubicin. |
| first_indexed | 2024-03-10T03:29:44Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:29:44Z |
| publishDate | 2021-12-01 |
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| series | Molecules |
| spelling | doaj.art-e03f16e267ef47e6bd718f80e9d2f2222023-11-23T09:45:12ZengMDPI AGMolecules1420-30492021-12-012624750910.3390/molecules26247509In Vitro Phytochemical Screening, Cytotoxicity Studies of <i>Curcuma longa</i> Extracts with Isolation and Characterisation of Their Isolated CompoundsMadhuri Grover0Tapan Behl1Aayush Sehgal2Sukhbir Singh3Neelam Sharma4Tarun Virmani5Mahesh Rachamalla6Abdullah Farasani7Sridevi Chigurupati8Amal M. Alsubayiel9Shatha Ghazi Felemban10Mohit Sanduja11Simona Bungau12School of Pharmaceutical Sciences, MVN University, Palwal 121102, IndiaChitkara College of Pharmacy, Chitkara University, Rajoura 140401, IndiaChitkara College of Pharmacy, Chitkara University, Rajoura 140401, IndiaChitkara College of Pharmacy, Chitkara University, Rajoura 140401, IndiaChitkara College of Pharmacy, Chitkara University, Rajoura 140401, IndiaSchool of Pharmaceutical Sciences, MVN University, Palwal 121102, IndiaDepartment of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, SK S7N 5E2, CanadaBiomedical Research Unit, Medical Research Centre, Jazan University, Jazan 45142, Saudi ArabiaDepartment of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraydah 52571, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Qassim University, Buraydah 52571, Saudi ArabiaDepartment of Medical Laboratory Science, Fakeeh College for Medical Sciences, Jeddah 21461, Saudi ArabiaSchool of Pharmaceutical Sciences, MVN University, Palwal 121102, IndiaDepartment of Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, 410073 Oradea, RomaniaThe <i>Curcuma longa</i> plant is endowed with multiple traditional and therapeutic utilities and is here explored for its phytochemical constituents and cytotoxic potential. Turmeric rhizomes were extracted from three different solvents and screened for the presence of different phytochemical constituents, observation of which indicated that the polar solvents favoured extraction of greater versatile phytochemical constituents. These extracts were investigated for their cytotoxic potential by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay on three different of cell lines including SCC-29B (oral cancer cell line), DU-145 (prostate cancer cell line) and the Vero cell line (healthy cell line/non-cancerous cell line). This assay was performed by taking three extracts from isolated curcuminoids and a pure bioactive compound bisdemethoxycurcumin (BD). Bisdemethoxycurcumin was isolated from curcuminoids and purified by column and thin-layer chromatography, and its structural characterisation was performed with different spectroscopic techniques such as FTIR, NMR (<sup>1</sup>H Proton and <sup>13</sup>C Carbon-NMR) and LC-MS. Amongst the extracts, the ethanolic extracts exhibited stronger cytotoxic potential against the oral cancer cell line (SCC-29B) with an IC<sub>50</sub>value of 11.27 μg/mL, and that this was too low of a cytotoxicity against the Vero cell line. Although, curcuminoids have also shown a comparable cytotoxic potential against SCC-29B (IC<sub>50</sub> value 16.79 μg/mL), it was not as potent against the ethanolic extract, and it was even found to be cytotoxic against healthy cell lines at a very low dose. While considering the isolated compound, bisdemethoxycurcumin, it also possessed a cytotoxic potential against the prostate cancer cell line (DU-145) (IC<sub>50</sub> value of 93.28 μg/mL), but was quite safe for the healthy cell line in comparison to doxorubicin.https://www.mdpi.com/1420-3049/26/24/7509<i>Curcuma longa</i>bisdemethoxycurcumincancercancer cell linescurcuminoidsethanolic extract |
| spellingShingle | Madhuri Grover Tapan Behl Aayush Sehgal Sukhbir Singh Neelam Sharma Tarun Virmani Mahesh Rachamalla Abdullah Farasani Sridevi Chigurupati Amal M. Alsubayiel Shatha Ghazi Felemban Mohit Sanduja Simona Bungau In Vitro Phytochemical Screening, Cytotoxicity Studies of <i>Curcuma longa</i> Extracts with Isolation and Characterisation of Their Isolated Compounds Molecules <i>Curcuma longa</i> bisdemethoxycurcumin cancer cancer cell lines curcuminoids ethanolic extract |
| title | In Vitro Phytochemical Screening, Cytotoxicity Studies of <i>Curcuma longa</i> Extracts with Isolation and Characterisation of Their Isolated Compounds |
| title_full | In Vitro Phytochemical Screening, Cytotoxicity Studies of <i>Curcuma longa</i> Extracts with Isolation and Characterisation of Their Isolated Compounds |
| title_fullStr | In Vitro Phytochemical Screening, Cytotoxicity Studies of <i>Curcuma longa</i> Extracts with Isolation and Characterisation of Their Isolated Compounds |
| title_full_unstemmed | In Vitro Phytochemical Screening, Cytotoxicity Studies of <i>Curcuma longa</i> Extracts with Isolation and Characterisation of Their Isolated Compounds |
| title_short | In Vitro Phytochemical Screening, Cytotoxicity Studies of <i>Curcuma longa</i> Extracts with Isolation and Characterisation of Their Isolated Compounds |
| title_sort | in vitro phytochemical screening cytotoxicity studies of i curcuma longa i extracts with isolation and characterisation of their isolated compounds |
| topic | <i>Curcuma longa</i> bisdemethoxycurcumin cancer cancer cell lines curcuminoids ethanolic extract |
| url | https://www.mdpi.com/1420-3049/26/24/7509 |
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