KLK10 exon 3 unmethylated PCR product concentration: a new potential early diagnostic marker in ovarian cancer? - A pilot study
Abstract Background KLK10 exon 3 hypermethylation correlated to tumor-specific lack of KLK10 expression in cancer cell lines and primary tumors. In the present study we investigate the possible role of KLK10 exon 3 methylation in ovarian tumor diagnosis and prognosis. Results Qualitative methylation...
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| Format: | Article |
| Language: | English |
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BMC
2018-04-01
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| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13048-018-0407-y |
| _version_ | 1797967405977174016 |
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| author | Mustafa A. El Sherbini Amal A. Mansour Maha M. Sallam Emtiaz A. Shaban Zeinab A. Shehab ElDin Amr H. El-Shalakany |
| author_facet | Mustafa A. El Sherbini Amal A. Mansour Maha M. Sallam Emtiaz A. Shaban Zeinab A. Shehab ElDin Amr H. El-Shalakany |
| author_sort | Mustafa A. El Sherbini |
| collection | DOAJ |
| description | Abstract Background KLK10 exon 3 hypermethylation correlated to tumor-specific lack of KLK10 expression in cancer cell lines and primary tumors. In the present study we investigate the possible role of KLK10 exon 3 methylation in ovarian tumor diagnosis and prognosis. Results Qualitative methylation-specific PCR (MSP) results did not show statistically significant differences in patient group samples (normal and tumor) where all samples were positive only for the unmethylated-specific PCR except for two malignant samples that were either doubly positive (serous carcinoma) or doubly negative (Sertoli-Leydig cell tumor) for the two MSP tests. However, KLK10 exon 3 unmethylated PCR product concentration (ng/μl) showed statistically significant differences in benign and malignant patient group samples; mean ± SD (n): tumor: 0.077 ± 0.035 (14) and 0.047 ± 0.021 (15), respectively, p-value = 0.011; and normal: 0.094 ± 0.039 (7) and 0.046 ± 0.027 (6), respectively, p-value = 0.031. Moreover, ROC curve analysis of KLK10 exon 3 unmethylated PCR product concentration in overall patient group samples showed good diagnostic ability (AUC = 0.778; p-value = 0.002). Patient survival (living and died) showed statistically significant difference according to preoperative serum CA125 concentration (U/ml); median (n): 101.25 (10) and 1252 (5), respectively, p-value = 0.037, but not KLK10 exon 3 unmethylated PCR product concentration (ng/μl) in overall malignant patient samples; mean ± SD (n): 0.042 ± 0.015 (14) and 0.055 ± 0.032 (7), p-value = 0.228. Conclusion To the best of our knowledge, this is the first report on KLK10 exon 3 unmethylated PCR product concentration as potential early epigenetic diagnostic marker in primary ovarian tumors. Taken into account the limitations in our study (small sample size and semi-quantitative PCR product analysis) further studies are strongly recommended. |
| first_indexed | 2024-04-11T02:30:44Z |
| format | Article |
| id | doaj.art-e03fa7d0361c43118a3ae4bd61829983 |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T02:30:44Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-e03fa7d0361c43118a3ae4bd618299832023-01-02T21:39:07ZengBMCJournal of Ovarian Research1757-22152018-04-0111111010.1186/s13048-018-0407-yKLK10 exon 3 unmethylated PCR product concentration: a new potential early diagnostic marker in ovarian cancer? - A pilot studyMustafa A. El Sherbini0Amal A. Mansour1Maha M. Sallam2Emtiaz A. Shaban3Zeinab A. Shehab ElDin4Amr H. El-Shalakany5Medical Biochemistry Department, Faculty of Medicine, Ain Shams UniversityMedical Biochemistry Department, Faculty of Medicine, Ain Shams UniversityMedical Biochemistry Department, Faculty of Medicine, Ain Shams UniversityMedical Biochemistry Department, Faculty of Medicine, Ain Shams UniversityGynecologic Oncology Unit, Ain Shams University Maternity HospitalGynecologic Oncology Unit, Ain Shams University Maternity HospitalAbstract Background KLK10 exon 3 hypermethylation correlated to tumor-specific lack of KLK10 expression in cancer cell lines and primary tumors. In the present study we investigate the possible role of KLK10 exon 3 methylation in ovarian tumor diagnosis and prognosis. Results Qualitative methylation-specific PCR (MSP) results did not show statistically significant differences in patient group samples (normal and tumor) where all samples were positive only for the unmethylated-specific PCR except for two malignant samples that were either doubly positive (serous carcinoma) or doubly negative (Sertoli-Leydig cell tumor) for the two MSP tests. However, KLK10 exon 3 unmethylated PCR product concentration (ng/μl) showed statistically significant differences in benign and malignant patient group samples; mean ± SD (n): tumor: 0.077 ± 0.035 (14) and 0.047 ± 0.021 (15), respectively, p-value = 0.011; and normal: 0.094 ± 0.039 (7) and 0.046 ± 0.027 (6), respectively, p-value = 0.031. Moreover, ROC curve analysis of KLK10 exon 3 unmethylated PCR product concentration in overall patient group samples showed good diagnostic ability (AUC = 0.778; p-value = 0.002). Patient survival (living and died) showed statistically significant difference according to preoperative serum CA125 concentration (U/ml); median (n): 101.25 (10) and 1252 (5), respectively, p-value = 0.037, but not KLK10 exon 3 unmethylated PCR product concentration (ng/μl) in overall malignant patient samples; mean ± SD (n): 0.042 ± 0.015 (14) and 0.055 ± 0.032 (7), p-value = 0.228. Conclusion To the best of our knowledge, this is the first report on KLK10 exon 3 unmethylated PCR product concentration as potential early epigenetic diagnostic marker in primary ovarian tumors. Taken into account the limitations in our study (small sample size and semi-quantitative PCR product analysis) further studies are strongly recommended.http://link.springer.com/article/10.1186/s13048-018-0407-yOvarian cancerCA125KLK10KLK6KLK10 exon 3 methylation |
| spellingShingle | Mustafa A. El Sherbini Amal A. Mansour Maha M. Sallam Emtiaz A. Shaban Zeinab A. Shehab ElDin Amr H. El-Shalakany KLK10 exon 3 unmethylated PCR product concentration: a new potential early diagnostic marker in ovarian cancer? - A pilot study Journal of Ovarian Research Ovarian cancer CA125 KLK10 KLK6 KLK10 exon 3 methylation |
| title | KLK10 exon 3 unmethylated PCR product concentration: a new potential early diagnostic marker in ovarian cancer? - A pilot study |
| title_full | KLK10 exon 3 unmethylated PCR product concentration: a new potential early diagnostic marker in ovarian cancer? - A pilot study |
| title_fullStr | KLK10 exon 3 unmethylated PCR product concentration: a new potential early diagnostic marker in ovarian cancer? - A pilot study |
| title_full_unstemmed | KLK10 exon 3 unmethylated PCR product concentration: a new potential early diagnostic marker in ovarian cancer? - A pilot study |
| title_short | KLK10 exon 3 unmethylated PCR product concentration: a new potential early diagnostic marker in ovarian cancer? - A pilot study |
| title_sort | klk10 exon 3 unmethylated pcr product concentration a new potential early diagnostic marker in ovarian cancer a pilot study |
| topic | Ovarian cancer CA125 KLK10 KLK6 KLK10 exon 3 methylation |
| url | http://link.springer.com/article/10.1186/s13048-018-0407-y |
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