Bioactivity and Bone Cell Formation with Poly-ε-Caprolactone/Bioceramic 3D Porous Scaffolds
This study applied poly-ε-caprolactone (PCL), a biomedical ceramic powder as an additive (nano-hydroxyapatite (nHA) or β-tricalcium diphosphate (β-TCP)), and sodium chloride (NaCl) and ammonium bicarbonate ((NH<sub>4</sub>)HCO<sub>3</sub>) as porogens; these stuffs were used...
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MDPI AG
2021-08-01
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author | Po-Kai Juan Fang-Yu Fan Wei-Chun Lin Pei-Bang Liao Chiung-Fang Huang Yung-Kang Shen Muhammad Ruslin Chen-Han Lee |
author_facet | Po-Kai Juan Fang-Yu Fan Wei-Chun Lin Pei-Bang Liao Chiung-Fang Huang Yung-Kang Shen Muhammad Ruslin Chen-Han Lee |
author_sort | Po-Kai Juan |
collection | DOAJ |
description | This study applied poly-ε-caprolactone (PCL), a biomedical ceramic powder as an additive (nano-hydroxyapatite (nHA) or β-tricalcium diphosphate (β-TCP)), and sodium chloride (NaCl) and ammonium bicarbonate ((NH<sub>4</sub>)HCO<sub>3</sub>) as porogens; these stuffs were used as scaffold materials. An improved solvent-casting/particulate-leaching method was utilized to fabricate 3D porous scaffolds. In this study we examined the physical properties (elastic modulus, porosity, and contact angle) and degradation properties (weight loss and pH value) of the 3D porous scaffolds. Both nHA and β-TCP improved the mechanical properties (elastic modulus) of the 3D porous scaffolds. The elastic modulus (0.15~1.865 GPa) of the various composite scaffolds matched that of human cancellous bone (0.1~4.5 GPa). Osteoblast-like (MG63) cells were cultured, a microculture tetrazolium test (MTT) was conducted and alkaline phosphatase (ALP) activity of the 3D porous scaffolds was determined. Experimental results indicated that both nHA and β-TCP powder improved the hydrophilic properties of the scaffolds. The degradation rate of the scaffolds was accelerated by adding nHA or β-TCP. The MTT and ALP activity tests indicated that the scaffolds with a high ratio of nHA or β-TCP had excellent properties of in vitro biocompatibility (cell attachment and proliferation). |
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issn | 2073-4360 |
language | English |
last_indexed | 2024-03-10T08:26:49Z |
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series | Polymers |
spelling | doaj.art-e0415266862b4503a8bdb141a240152b2023-11-22T09:23:20ZengMDPI AGPolymers2073-43602021-08-011316271810.3390/polym13162718Bioactivity and Bone Cell Formation with Poly-ε-Caprolactone/Bioceramic 3D Porous ScaffoldsPo-Kai Juan0Fang-Yu Fan1Wei-Chun Lin2Pei-Bang Liao3Chiung-Fang Huang4Yung-Kang Shen5Muhammad Ruslin6Chen-Han Lee7Division of Prosthodontics, Department of Dentistry, Taipei Medical University Hospital, Taipei 11031, TaiwanSchool of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 11031, TaiwanSchool of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 11031, TaiwanDivision of Prosthodontics, Department of Dentistry, Taipei Medical University Hospital, Taipei 11031, TaiwanSchool of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 11031, TaiwanSchool of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 11031, TaiwanDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, Hasanuddin University, Makassar 90245, IndonesiaTaiwan Society of Blood Biomaterials, New Taipei City 221, TaiwanThis study applied poly-ε-caprolactone (PCL), a biomedical ceramic powder as an additive (nano-hydroxyapatite (nHA) or β-tricalcium diphosphate (β-TCP)), and sodium chloride (NaCl) and ammonium bicarbonate ((NH<sub>4</sub>)HCO<sub>3</sub>) as porogens; these stuffs were used as scaffold materials. An improved solvent-casting/particulate-leaching method was utilized to fabricate 3D porous scaffolds. In this study we examined the physical properties (elastic modulus, porosity, and contact angle) and degradation properties (weight loss and pH value) of the 3D porous scaffolds. Both nHA and β-TCP improved the mechanical properties (elastic modulus) of the 3D porous scaffolds. The elastic modulus (0.15~1.865 GPa) of the various composite scaffolds matched that of human cancellous bone (0.1~4.5 GPa). Osteoblast-like (MG63) cells were cultured, a microculture tetrazolium test (MTT) was conducted and alkaline phosphatase (ALP) activity of the 3D porous scaffolds was determined. Experimental results indicated that both nHA and β-TCP powder improved the hydrophilic properties of the scaffolds. The degradation rate of the scaffolds was accelerated by adding nHA or β-TCP. The MTT and ALP activity tests indicated that the scaffolds with a high ratio of nHA or β-TCP had excellent properties of in vitro biocompatibility (cell attachment and proliferation).https://www.mdpi.com/2073-4360/13/16/27183D composite scaffoldimproved solvent-casting/particulate-leachingbioceramicphysical and degradation propertiesbiocompatibility |
spellingShingle | Po-Kai Juan Fang-Yu Fan Wei-Chun Lin Pei-Bang Liao Chiung-Fang Huang Yung-Kang Shen Muhammad Ruslin Chen-Han Lee Bioactivity and Bone Cell Formation with Poly-ε-Caprolactone/Bioceramic 3D Porous Scaffolds Polymers 3D composite scaffold improved solvent-casting/particulate-leaching bioceramic physical and degradation properties biocompatibility |
title | Bioactivity and Bone Cell Formation with Poly-ε-Caprolactone/Bioceramic 3D Porous Scaffolds |
title_full | Bioactivity and Bone Cell Formation with Poly-ε-Caprolactone/Bioceramic 3D Porous Scaffolds |
title_fullStr | Bioactivity and Bone Cell Formation with Poly-ε-Caprolactone/Bioceramic 3D Porous Scaffolds |
title_full_unstemmed | Bioactivity and Bone Cell Formation with Poly-ε-Caprolactone/Bioceramic 3D Porous Scaffolds |
title_short | Bioactivity and Bone Cell Formation with Poly-ε-Caprolactone/Bioceramic 3D Porous Scaffolds |
title_sort | bioactivity and bone cell formation with poly ε caprolactone bioceramic 3d porous scaffolds |
topic | 3D composite scaffold improved solvent-casting/particulate-leaching bioceramic physical and degradation properties biocompatibility |
url | https://www.mdpi.com/2073-4360/13/16/2718 |
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