The Antiamoebic Effect of Oliveria Decumbens and Peganum Harmala Extract on Acanthamoeba
Background and Aim Acanthamoeba is one of free-living amoebas, which are very abundant in nature. As a free-living amoeba, this parasite has a very high lethality, especially in people with underlying diseases, so researchers are always looking for a way to combat it. Drug plants are a good way to f...
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| Format: | Article |
| Language: | fas |
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Arak Medical University
2022-08-01
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| Series: | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| Subjects: | |
| Online Access: | http://jams.arakmu.ac.ir/article-1-7298-en.pdf |
| _version_ | 1797810456258150400 |
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| author | Gholamreza Shookohi Naser Hatami Omid Mojarad Ahmad Abolghazi |
| author_facet | Gholamreza Shookohi Naser Hatami Omid Mojarad Ahmad Abolghazi |
| author_sort | Gholamreza Shookohi |
| collection | DOAJ |
| description | Background and Aim Acanthamoeba is one of free-living amoebas, which are very abundant in nature. As a free-living amoeba, this parasite has a very high lethality, especially in people with underlying diseases, so researchers are always looking for a way to combat it. Drug plants are a good way to fight Acanthamoeba species. In this study, we aimed to investigate the lethal effect of the extract of Oliveria decumbens vent and Peganum harmala alcoholic extract on Acanthamoeba.
Methods & Materials In this study using the extract from an extract of Oliveria decumbens vent and Peganum harmala with concentrations of 1.25, 2.5, 5, 10, and 20 mg/ml to investigate the lethal effect of this extract. The plant was treated with Acanthamoeba amoebae after three times (24, 48, and 72) hours.
Ethical Considerations This study was approved by the Ethics Committee of the Jahrom University of Medical Sciences (Code: IR.JUMS.REC.1398.029).
Results The present research showed that using different concentrations at three times (24, 48, and 72) hours the effect of the extract on trophozoites and cysts of Acanthamoeba was shown. The highest lethality is related to the concentration of 20 mg/ml using a combination of both extracts at the time 72 hours and the lowest lethality is related to the concentration of 1.25 mg/ml of Oliveria decumbens vent at the time 24 hours.
Conclusion Observations indicate that the alcoholic extract of Oliveria decumbens vent and Peganum harmala had a perfect effect both separately and in a combination of both extracts. These two extracts had a synergistic effect on the lethal effect of Acanthamoeba amoeba. |
| first_indexed | 2024-03-13T07:08:04Z |
| format | Article |
| id | doaj.art-e0424ae7fb064e45966ec9e844389934 |
| institution | Directory Open Access Journal |
| issn | 1735-5338 2008-644X |
| language | fas |
| last_indexed | 2024-03-13T07:08:04Z |
| publishDate | 2022-08-01 |
| publisher | Arak Medical University |
| record_format | Article |
| series | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| spelling | doaj.art-e0424ae7fb064e45966ec9e8443899342023-06-06T07:24:42ZfasArak Medical UniversityMajallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk1735-53382008-644X2022-08-01253372381The Antiamoebic Effect of Oliveria Decumbens and Peganum Harmala Extract on AcanthamoebaGholamreza Shookohi0Naser Hatami1Omid Mojarad2Ahmad Abolghazi3 Department of Medical Parasitology and Mycology, Faculty of Medical, Jahrom University of Medical Sciences, Jahrom, Iran. Department of Medical Parasitology and Mycology, Faculty of Medical, Jahrom University of Medical Sciences, Jahrom, Iran. Department of Medical Parasitology and Mycology, Faculty of Medical, Jahrom University of Medical Sciences, Jahrom, Iran. Department of Medical Parasitology and Mycology, Faculty of Medical, Jahrom University of Medical Sciences, Jahrom, Iran., Department of Medical Parasitology and Mycology, Faculty of Medical, Hamedan University of Medical Sciences, Hamedan, Iran. Background and Aim Acanthamoeba is one of free-living amoebas, which are very abundant in nature. As a free-living amoeba, this parasite has a very high lethality, especially in people with underlying diseases, so researchers are always looking for a way to combat it. Drug plants are a good way to fight Acanthamoeba species. In this study, we aimed to investigate the lethal effect of the extract of Oliveria decumbens vent and Peganum harmala alcoholic extract on Acanthamoeba. Methods & Materials In this study using the extract from an extract of Oliveria decumbens vent and Peganum harmala with concentrations of 1.25, 2.5, 5, 10, and 20 mg/ml to investigate the lethal effect of this extract. The plant was treated with Acanthamoeba amoebae after three times (24, 48, and 72) hours. Ethical Considerations This study was approved by the Ethics Committee of the Jahrom University of Medical Sciences (Code: IR.JUMS.REC.1398.029). Results The present research showed that using different concentrations at three times (24, 48, and 72) hours the effect of the extract on trophozoites and cysts of Acanthamoeba was shown. The highest lethality is related to the concentration of 20 mg/ml using a combination of both extracts at the time 72 hours and the lowest lethality is related to the concentration of 1.25 mg/ml of Oliveria decumbens vent at the time 24 hours. Conclusion Observations indicate that the alcoholic extract of Oliveria decumbens vent and Peganum harmala had a perfect effect both separately and in a combination of both extracts. These two extracts had a synergistic effect on the lethal effect of Acanthamoeba amoeba.http://jams.arakmu.ac.ir/article-1-7298-en.pdfamoebaoliveria decumbenspeganum harmalaacanthamoeba |
| spellingShingle | Gholamreza Shookohi Naser Hatami Omid Mojarad Ahmad Abolghazi The Antiamoebic Effect of Oliveria Decumbens and Peganum Harmala Extract on Acanthamoeba Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk amoeba oliveria decumbens peganum harmala acanthamoeba |
| title | The Antiamoebic Effect of Oliveria Decumbens and Peganum Harmala Extract on Acanthamoeba |
| title_full | The Antiamoebic Effect of Oliveria Decumbens and Peganum Harmala Extract on Acanthamoeba |
| title_fullStr | The Antiamoebic Effect of Oliveria Decumbens and Peganum Harmala Extract on Acanthamoeba |
| title_full_unstemmed | The Antiamoebic Effect of Oliveria Decumbens and Peganum Harmala Extract on Acanthamoeba |
| title_short | The Antiamoebic Effect of Oliveria Decumbens and Peganum Harmala Extract on Acanthamoeba |
| title_sort | antiamoebic effect of oliveria decumbens and peganum harmala extract on acanthamoeba |
| topic | amoeba oliveria decumbens peganum harmala acanthamoeba |
| url | http://jams.arakmu.ac.ir/article-1-7298-en.pdf |
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