Insemination of bocachico fish (Prochilodus magdalenae) with fresh or cryopreserved semen: effect of spermatozoa/oocyte ratio
Background: cryopreservation is an important biotechnological tool in the conservation of biodiversity, particularly for endangered species. Objective: to evaluate six different spermatozoa/oocyte ratios using fresh and cryopreserved semen in bocachico fish (Prochilodus magdalenae). Methods: fresh s...
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| Format: | Article |
| Language: | English |
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Universidad de Antioquia
2015-12-01
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| Series: | Revista Colombiana de Ciencias Pecuarias |
| Subjects: | |
| Online Access: | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-06902015000400008&lng=en&tlng=en |
| _version_ | 1824044501021229056 |
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| author | Víctor J Atencio García José A Espinosa José G Martínez Sandra C Pardo Carrasco |
| author_facet | Víctor J Atencio García José A Espinosa José G Martínez Sandra C Pardo Carrasco |
| author_sort | Víctor J Atencio García |
| collection | DOAJ |
| description | Background: cryopreservation is an important biotechnological tool in the conservation of biodiversity, particularly for endangered species. Objective: to evaluate six different spermatozoa/oocyte ratios using fresh and cryopreserved semen in bocachico fish (Prochilodus magdalenae). Methods: fresh semen was collected and its quality determined to verify cryopreservation feasibility. The semen was put in 5 mL straws and mixed with a solution (5.5% glucose, 12% egg yolk, and 10% dimethyl sulfoxide -DMSO-) in a 1:4 dilution (semen:solution). The semen was frozen in nitrogen vapor dry shipper for 30 min, rapidly transferred to storage thermos, and submerged directly into liquid nitrogen (LN; -196 °C). Straws were thawed at 60 ºC for 45 seconds. Motility, velocity, and sperm progressivity of fresh and cryopreserved semen were assessed using Sperm Class Analyzer (SCA®) software. Each proportion of spermatozoa/oocyte was assessed with 2 g of eggs (1,630 ± 87 eggs/g) to evaluate fertility (F), hatching (H), and larval survival (LS) rates. Results: the best reproductive performance for fresh semen was obtained inseminating with 160,000 spermatozoa/oocyte (F = 75.0%, H = 67.7%, LS = 32.7%). Similarly, the best reproductive performance for cryopreserved semen was achieved with 320,000 spermatozoa/oocyte (F = 70.0%, H = 48.6%, LS = 19.5%). Conclusion: it is possible to achieve adequate reproductive performance in bocachico fish using cryopreserved sperm (10% DMSO, 5.5% glucose, and 12% egg yolk) at twice the spermatozoa/oocyte ratio used with fresh semen. |
| first_indexed | 2024-12-23T00:12:18Z |
| format | Article |
| id | doaj.art-e0437687efeb487aa4510e913676bfbd |
| institution | Directory Open Access Journal |
| issn | 0120-0690 |
| language | English |
| last_indexed | 2024-12-23T00:12:18Z |
| publishDate | 2015-12-01 |
| publisher | Universidad de Antioquia |
| record_format | Article |
| series | Revista Colombiana de Ciencias Pecuarias |
| spelling | doaj.art-e0437687efeb487aa4510e913676bfbd2022-12-21T18:07:30ZengUniversidad de AntioquiaRevista Colombiana de Ciencias Pecuarias0120-06902015-12-0128434735510.17533/udea.rccp.v28n4a07S0120-06902015000400008Insemination of bocachico fish (Prochilodus magdalenae) with fresh or cryopreserved semen: effect of spermatozoa/oocyte ratioVíctor J Atencio García0José A Espinosa1José G Martínez2Sandra C Pardo Carrasco3Universidad de CórdobaUniversidad de CórdobaFederal University of AmazonasUniversidad Nacional de Colombia Sede MedellínBackground: cryopreservation is an important biotechnological tool in the conservation of biodiversity, particularly for endangered species. Objective: to evaluate six different spermatozoa/oocyte ratios using fresh and cryopreserved semen in bocachico fish (Prochilodus magdalenae). Methods: fresh semen was collected and its quality determined to verify cryopreservation feasibility. The semen was put in 5 mL straws and mixed with a solution (5.5% glucose, 12% egg yolk, and 10% dimethyl sulfoxide -DMSO-) in a 1:4 dilution (semen:solution). The semen was frozen in nitrogen vapor dry shipper for 30 min, rapidly transferred to storage thermos, and submerged directly into liquid nitrogen (LN; -196 °C). Straws were thawed at 60 ºC for 45 seconds. Motility, velocity, and sperm progressivity of fresh and cryopreserved semen were assessed using Sperm Class Analyzer (SCA®) software. Each proportion of spermatozoa/oocyte was assessed with 2 g of eggs (1,630 ± 87 eggs/g) to evaluate fertility (F), hatching (H), and larval survival (LS) rates. Results: the best reproductive performance for fresh semen was obtained inseminating with 160,000 spermatozoa/oocyte (F = 75.0%, H = 67.7%, LS = 32.7%). Similarly, the best reproductive performance for cryopreserved semen was achieved with 320,000 spermatozoa/oocyte (F = 70.0%, H = 48.6%, LS = 19.5%). Conclusion: it is possible to achieve adequate reproductive performance in bocachico fish using cryopreserved sperm (10% DMSO, 5.5% glucose, and 12% egg yolk) at twice the spermatozoa/oocyte ratio used with fresh semen.http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-06902015000400008&lng=en&tlng=encriobiologíafertilidadProchilontidaereproducción artificial |
| spellingShingle | Víctor J Atencio García José A Espinosa José G Martínez Sandra C Pardo Carrasco Insemination of bocachico fish (Prochilodus magdalenae) with fresh or cryopreserved semen: effect of spermatozoa/oocyte ratio Revista Colombiana de Ciencias Pecuarias criobiología fertilidad Prochilontidae reproducción artificial |
| title | Insemination of bocachico fish (Prochilodus magdalenae) with fresh or cryopreserved semen: effect of spermatozoa/oocyte ratio |
| title_full | Insemination of bocachico fish (Prochilodus magdalenae) with fresh or cryopreserved semen: effect of spermatozoa/oocyte ratio |
| title_fullStr | Insemination of bocachico fish (Prochilodus magdalenae) with fresh or cryopreserved semen: effect of spermatozoa/oocyte ratio |
| title_full_unstemmed | Insemination of bocachico fish (Prochilodus magdalenae) with fresh or cryopreserved semen: effect of spermatozoa/oocyte ratio |
| title_short | Insemination of bocachico fish (Prochilodus magdalenae) with fresh or cryopreserved semen: effect of spermatozoa/oocyte ratio |
| title_sort | insemination of bocachico fish prochilodus magdalenae with fresh or cryopreserved semen effect of spermatozoa oocyte ratio |
| topic | criobiología fertilidad Prochilontidae reproducción artificial |
| url | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-06902015000400008&lng=en&tlng=en |
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