Overexpression of <i>OsCASP1</i> Improves Calcium Tolerance in Rice
The Casparian strip domain protein 1 (<i>OsCASP1</i>) is necessary for the formation of the Casparian strip (CS) in the rice endodermis. It also controls Ca<sup>2+</sup> transport to the stele. Here, we demonstrated that <i>OsCASP1</i> overexpression enhanced Ca t...
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| author | Zhigang Wang Zhiwei Chen Xiang Zhang Qiuxing Wei Yafeng Xin Baolei Zhang Fuhang Liu Jixing Xia |
| author_facet | Zhigang Wang Zhiwei Chen Xiang Zhang Qiuxing Wei Yafeng Xin Baolei Zhang Fuhang Liu Jixing Xia |
| author_sort | Zhigang Wang |
| collection | DOAJ |
| description | The Casparian strip domain protein 1 (<i>OsCASP1</i>) is necessary for the formation of the Casparian strip (CS) in the rice endodermis. It also controls Ca<sup>2+</sup> transport to the stele. Here, we demonstrated that <i>OsCASP1</i> overexpression enhanced Ca tolerance in rice. Under normal conditions, <i>OsCASP1</i>-overexpressed lines showed similar concentrations of essential metals in the roots and shoots compared to the wild type, while under high Ca conditions, Ca in the roots, shoots, and xylem sap of the <i>OsCASP1</i>-overexpressed lines was significantly decreased. This did not apply to other essential metals. Ca-inhibited growth was significantly alleviated in the <i>OsCASP1</i>-overexpressed lines. Furthermore, <i>OsCASP1</i> overexpression resulted in earlier formation of both the CS and functional apoplastic barrier in the endodermis but did not induce ectopic CS formation in non-endodermal cell layers and affect suberin accumulation in the endodermis. These results indicate that the overexpression of <i>OsCASP1</i> promotes CS formation in endodermal cells and inhibits Ca<sup>2+</sup> transport by the apoplastic pathway, restricting Ca accumulation in the roots and shoots under high Ca conditions. Taken together, the results suggest that <i>OsCASP1</i> overexpression is an effective way to improve rice adaptation to high Ca environments. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:48:14Z |
| publishDate | 2021-06-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e04466497d964b589945593673c5d07d2023-11-21T22:28:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012211600210.3390/ijms22116002Overexpression of <i>OsCASP1</i> Improves Calcium Tolerance in RiceZhigang Wang0Zhiwei Chen1Xiang Zhang2Qiuxing Wei3Yafeng Xin4Baolei Zhang5Fuhang Liu6Jixing Xia7State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaThe Casparian strip domain protein 1 (<i>OsCASP1</i>) is necessary for the formation of the Casparian strip (CS) in the rice endodermis. It also controls Ca<sup>2+</sup> transport to the stele. Here, we demonstrated that <i>OsCASP1</i> overexpression enhanced Ca tolerance in rice. Under normal conditions, <i>OsCASP1</i>-overexpressed lines showed similar concentrations of essential metals in the roots and shoots compared to the wild type, while under high Ca conditions, Ca in the roots, shoots, and xylem sap of the <i>OsCASP1</i>-overexpressed lines was significantly decreased. This did not apply to other essential metals. Ca-inhibited growth was significantly alleviated in the <i>OsCASP1</i>-overexpressed lines. Furthermore, <i>OsCASP1</i> overexpression resulted in earlier formation of both the CS and functional apoplastic barrier in the endodermis but did not induce ectopic CS formation in non-endodermal cell layers and affect suberin accumulation in the endodermis. These results indicate that the overexpression of <i>OsCASP1</i> promotes CS formation in endodermal cells and inhibits Ca<sup>2+</sup> transport by the apoplastic pathway, restricting Ca accumulation in the roots and shoots under high Ca conditions. Taken together, the results suggest that <i>OsCASP1</i> overexpression is an effective way to improve rice adaptation to high Ca environments.https://www.mdpi.com/1422-0067/22/11/6002Casparian stripsuberin lamellaeapoplastic barriercalciumlignin |
| spellingShingle | Zhigang Wang Zhiwei Chen Xiang Zhang Qiuxing Wei Yafeng Xin Baolei Zhang Fuhang Liu Jixing Xia Overexpression of <i>OsCASP1</i> Improves Calcium Tolerance in Rice International Journal of Molecular Sciences Casparian strip suberin lamellae apoplastic barrier calcium lignin |
| title | Overexpression of <i>OsCASP1</i> Improves Calcium Tolerance in Rice |
| title_full | Overexpression of <i>OsCASP1</i> Improves Calcium Tolerance in Rice |
| title_fullStr | Overexpression of <i>OsCASP1</i> Improves Calcium Tolerance in Rice |
| title_full_unstemmed | Overexpression of <i>OsCASP1</i> Improves Calcium Tolerance in Rice |
| title_short | Overexpression of <i>OsCASP1</i> Improves Calcium Tolerance in Rice |
| title_sort | overexpression of i oscasp1 i improves calcium tolerance in rice |
| topic | Casparian strip suberin lamellae apoplastic barrier calcium lignin |
| url | https://www.mdpi.com/1422-0067/22/11/6002 |
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