A single intravenous AAV9 injection mediates bilateral gene transfer to the adult mouse retina.
Widespread gene delivery to the retina is an important challenge for the treatment of retinal diseases, such as retinal dystrophies. We and others have recently shown that the intravenous injection of a self-complementary (sc) AAV9 vector can direct efficient cell transduction in the central nervous...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3626698?pdf=render |
| _version_ | 1811264670257905664 |
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| author | Alexis-Pierre Bemelmans Sandra Duqué Christel Rivière Stéphanie Astord Mélissa Desrosiers Thibault Marais José-Alain Sahel Thomas Voit Martine Barkats |
| author_facet | Alexis-Pierre Bemelmans Sandra Duqué Christel Rivière Stéphanie Astord Mélissa Desrosiers Thibault Marais José-Alain Sahel Thomas Voit Martine Barkats |
| author_sort | Alexis-Pierre Bemelmans |
| collection | DOAJ |
| description | Widespread gene delivery to the retina is an important challenge for the treatment of retinal diseases, such as retinal dystrophies. We and others have recently shown that the intravenous injection of a self-complementary (sc) AAV9 vector can direct efficient cell transduction in the central nervous system, in both neonatal and adult animals. We show here that the intravenous injection of scAAV9 encoding green fluorescent protein (GFP) resulted in gene transfer to all layers of the retina in adult mice, despite the presence of a mature blood-eye barrier. Cell morphology studies and double-labeling with retinal cell-specific markers showed that GFP was expressed in retinal pigment epithelium cells, photoreceptors, bipolar cells, Müller cells and retinal ganglion cells. The cells on the inner side of the retina, including retinal ganglion cells in particular, were transduced with the highest efficiency. Quantification of the cell population co-expressing GFP and Brn-3a showed that 45% of the retinal ganglion cells were efficiently transduced after intravenous scAAV9-GFP injection in adult mice. This study provides the first demonstration that a single intravenous scAAV9 injection can deliver transgenes to the retinas of both eyes in adult mice, suggesting that this vector serotype is able to cross mature blood-eye barriers. This intravascular gene transfer approach, by eliminating the potential invasiveness of ocular surgery, could constitute an alternative when fragility of the retina precludes subretinal or intravitreal injections of viral vectors, opening up new possibilities for gene therapy for retinal diseases. |
| first_indexed | 2024-04-12T20:08:32Z |
| format | Article |
| id | doaj.art-e048910f81a046eda072ae57275f1bdd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T20:08:32Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e048910f81a046eda072ae57275f1bdd2022-12-22T03:18:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6161810.1371/journal.pone.0061618A single intravenous AAV9 injection mediates bilateral gene transfer to the adult mouse retina.Alexis-Pierre BemelmansSandra DuquéChristel RivièreStéphanie AstordMélissa DesrosiersThibault MaraisJosé-Alain SahelThomas VoitMartine BarkatsWidespread gene delivery to the retina is an important challenge for the treatment of retinal diseases, such as retinal dystrophies. We and others have recently shown that the intravenous injection of a self-complementary (sc) AAV9 vector can direct efficient cell transduction in the central nervous system, in both neonatal and adult animals. We show here that the intravenous injection of scAAV9 encoding green fluorescent protein (GFP) resulted in gene transfer to all layers of the retina in adult mice, despite the presence of a mature blood-eye barrier. Cell morphology studies and double-labeling with retinal cell-specific markers showed that GFP was expressed in retinal pigment epithelium cells, photoreceptors, bipolar cells, Müller cells and retinal ganglion cells. The cells on the inner side of the retina, including retinal ganglion cells in particular, were transduced with the highest efficiency. Quantification of the cell population co-expressing GFP and Brn-3a showed that 45% of the retinal ganglion cells were efficiently transduced after intravenous scAAV9-GFP injection in adult mice. This study provides the first demonstration that a single intravenous scAAV9 injection can deliver transgenes to the retinas of both eyes in adult mice, suggesting that this vector serotype is able to cross mature blood-eye barriers. This intravascular gene transfer approach, by eliminating the potential invasiveness of ocular surgery, could constitute an alternative when fragility of the retina precludes subretinal or intravitreal injections of viral vectors, opening up new possibilities for gene therapy for retinal diseases.http://europepmc.org/articles/PMC3626698?pdf=render |
| spellingShingle | Alexis-Pierre Bemelmans Sandra Duqué Christel Rivière Stéphanie Astord Mélissa Desrosiers Thibault Marais José-Alain Sahel Thomas Voit Martine Barkats A single intravenous AAV9 injection mediates bilateral gene transfer to the adult mouse retina. PLoS ONE |
| title | A single intravenous AAV9 injection mediates bilateral gene transfer to the adult mouse retina. |
| title_full | A single intravenous AAV9 injection mediates bilateral gene transfer to the adult mouse retina. |
| title_fullStr | A single intravenous AAV9 injection mediates bilateral gene transfer to the adult mouse retina. |
| title_full_unstemmed | A single intravenous AAV9 injection mediates bilateral gene transfer to the adult mouse retina. |
| title_short | A single intravenous AAV9 injection mediates bilateral gene transfer to the adult mouse retina. |
| title_sort | single intravenous aav9 injection mediates bilateral gene transfer to the adult mouse retina |
| url | http://europepmc.org/articles/PMC3626698?pdf=render |
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