| Summary: | The appearance of <i>Klebsiella pneumoniae</i> strains producing extended-spectrum β-lactamase (ESBL), and carbapenemase (KPC) has turned into a significant public health issue. ESBL- and KPC-producing <i>K. pneumoniae</i>’s ability to form biofilms is a significant concern as it can promote the spread of antibiotic resistance and prolong infections in healthcare facilities. A total of 45 <i>K. pneumoniae</i> strains were isolated from human infections. Antibiograms were performed for 17 antibiotics, ESBL production was tested by Etest ESBL PM/PML, a rapid test was used to detect KPC carbapenemases, and resistance genes were detected by PCR. Biofilm production was detected by the microtiter plate method. A total of 73% of multidrug resistance was found, with the highest resistance rates to ampicillin, trimethoprim–sulfamethoxazole, cefotaxime, amoxicillin-clavulanic acid, and aztreonam. Simultaneously, the most effective antibiotics were tetracycline and amikacin. <i>bla<sub>CTX-M</sub></i>, <i>bla<sub>TEM</sub></i>, <i>bla<sub>SHV</sub></i>, <i>aac(3)-II</i>, <i>aadA1</i>, <i>tetA</i>, <i>cmlA</i>, <i>catA</i>, <i>gyrA</i>, <i>gyrB</i>, <i>parC</i>, <i>sul1</i>, <i>sul2</i>, <i>sul3</i>, <i>bla<sub>KPC</sub></i>, <i>bla<sub>OXA</sub></i>, and <i>bla<sub>PER</sub></i> genes were detected. Biofilm production showed that 80% of <i>K. pneumoniae</i> strains were biofilm producers. Most ESBL- and KPC-producing isolates were weak biofilm producers (40.0% and 60.0%, respectively). There was no correlation between the ability to form stronger biofilms and the presence of ESBL and KPC enzymes in <i>K. pneumoniae</i> isolates.
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