Orthohantaviruses in Reservoir and Atypical Hosts in the Czech Republic: Spillover Infection and Indication of Virus-Specific Tissue Tropism
ABSTRACT Orthohantaviruses (genus Orthohantavirus) are a diverse group of viruses that are closely associated with their natural hosts (rodents, shrews, and moles). Several orthohantaviruses cause severe disease in humans. Central and western Europe are areas with emerging orthohantavirus occurrence...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2022-10-01
|
| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.01306-22 |
| _version_ | 1811198197987540992 |
|---|---|
| author | Václav Hönig Jan Kamiš Aneta Maršíková Tereza Matějková Pavel Stopka Anna Mácová Daniel Růžek Jana Kvičerová |
| author_facet | Václav Hönig Jan Kamiš Aneta Maršíková Tereza Matějková Pavel Stopka Anna Mácová Daniel Růžek Jana Kvičerová |
| author_sort | Václav Hönig |
| collection | DOAJ |
| description | ABSTRACT Orthohantaviruses (genus Orthohantavirus) are a diverse group of viruses that are closely associated with their natural hosts (rodents, shrews, and moles). Several orthohantaviruses cause severe disease in humans. Central and western Europe are areas with emerging orthohantavirus occurrences. In our study, several orthohantaviruses, including the pathogenic Kurkino virus (KURV), were detected in their natural hosts trapped at several study sites in the Czech Republic. KURV was detected mainly in its typical host, the striped field mouse (Apodemus agrarius). Nevertheless, spillover infections were also detected in wood mice (Apodemus sylvaticus) and common voles (Microtus arvalis). Similarly, Tula virus (TULV) was found primarily in common voles, and events of spillover to rodents of other host species, including Apodemus spp., were recorded. In addition, unlike most previous studies, different tissues were sampled and compared to assess their suitability for orthohantavirus screening and possible tissue tropism. Our data suggest possible virus-specific tissue tropism in rodent hosts. TULV was most commonly detected in the lung tissue, whereas KURV was more common in the liver, spleen, and brain. Moreover, Seewis and Asikkala viruses were detected in randomly found common shrews (Sorex araneus). In conclusion, we have demonstrated the presence of human-pathogenic KURV and the potentially pathogenic TULV in their typical hosts as well as their spillover to atypical host species belonging to another family. Furthermore, we suggest the possibility of virus-specific tissue tropism of orthohantaviruses in their natural hosts. IMPORTANCE Orthohantaviruses (genus Orthohantavirus, family Hantaviridae) are a diverse group of globally distributed viruses that are closely associated with their natural hosts. Some orthohantaviruses are capable of infecting humans and causing severe disease. Orthohantaviruses are considered emerging pathogens due to their ever-increasing diversity and increasing numbers of disease cases. We report the detection of four different orthohantaviruses in rodents and shrews in the Czech Republic. Most viruses were found in their typical hosts, Kurkino virus (KURV) in striped field mice (Apodemus agrarius), Tula virus (TULV) in common voles (Microtus arvalis), and Seewis virus in common shrews (Sorex araneus). Nevertheless, spillover infections of atypical host species were also recorded for KURV, TULV, and another shrew-borne orthohantavirus, Asikkala virus. In addition, indications of virus-specific patterns of tissue tropism were observed. Our results highlight the circulation of several orthohantaviruses, including KURV, which is pathogenic to humans, among rodents and shrews in the Czech Republic. |
| first_indexed | 2024-04-12T01:26:43Z |
| format | Article |
| id | doaj.art-e04fdb0579ca461abaf6204197181377 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-04-12T01:26:43Z |
| publishDate | 2022-10-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-e04fdb0579ca461abaf62041971813772022-12-22T03:53:38ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972022-10-0110510.1128/spectrum.01306-22Orthohantaviruses in Reservoir and Atypical Hosts in the Czech Republic: Spillover Infection and Indication of Virus-Specific Tissue TropismVáclav Hönig0Jan Kamiš1Aneta Maršíková2Tereza Matějková3Pavel Stopka4Anna Mácová5Daniel Růžek6Jana Kvičerová7Laboratory of Arbovirology, Institute of Parasitology, Biology Centre CAS, České Budějovice, Czech RepublicLaboratory of Arbovirology, Institute of Parasitology, Biology Centre CAS, České Budějovice, Czech RepublicDepartment of Parasitology, Faculty of Science, University of South Bohemia, České Budějovice, Czech RepublicDepartment of Zoology, Faculty of Science, Charles University, Biocev, Vestec, Czech RepublicDepartment of Zoology, Faculty of Science, Charles University, Biocev, Vestec, Czech RepublicDepartment of Parasitology, Faculty of Science, University of South Bohemia, České Budějovice, Czech RepublicLaboratory of Arbovirology, Institute of Parasitology, Biology Centre CAS, České Budějovice, Czech RepublicDepartment of Parasitology, Faculty of Science, University of South Bohemia, České Budějovice, Czech RepublicABSTRACT Orthohantaviruses (genus Orthohantavirus) are a diverse group of viruses that are closely associated with their natural hosts (rodents, shrews, and moles). Several orthohantaviruses cause severe disease in humans. Central and western Europe are areas with emerging orthohantavirus occurrences. In our study, several orthohantaviruses, including the pathogenic Kurkino virus (KURV), were detected in their natural hosts trapped at several study sites in the Czech Republic. KURV was detected mainly in its typical host, the striped field mouse (Apodemus agrarius). Nevertheless, spillover infections were also detected in wood mice (Apodemus sylvaticus) and common voles (Microtus arvalis). Similarly, Tula virus (TULV) was found primarily in common voles, and events of spillover to rodents of other host species, including Apodemus spp., were recorded. In addition, unlike most previous studies, different tissues were sampled and compared to assess their suitability for orthohantavirus screening and possible tissue tropism. Our data suggest possible virus-specific tissue tropism in rodent hosts. TULV was most commonly detected in the lung tissue, whereas KURV was more common in the liver, spleen, and brain. Moreover, Seewis and Asikkala viruses were detected in randomly found common shrews (Sorex araneus). In conclusion, we have demonstrated the presence of human-pathogenic KURV and the potentially pathogenic TULV in their typical hosts as well as their spillover to atypical host species belonging to another family. Furthermore, we suggest the possibility of virus-specific tissue tropism of orthohantaviruses in their natural hosts. IMPORTANCE Orthohantaviruses (genus Orthohantavirus, family Hantaviridae) are a diverse group of globally distributed viruses that are closely associated with their natural hosts. Some orthohantaviruses are capable of infecting humans and causing severe disease. Orthohantaviruses are considered emerging pathogens due to their ever-increasing diversity and increasing numbers of disease cases. We report the detection of four different orthohantaviruses in rodents and shrews in the Czech Republic. Most viruses were found in their typical hosts, Kurkino virus (KURV) in striped field mice (Apodemus agrarius), Tula virus (TULV) in common voles (Microtus arvalis), and Seewis virus in common shrews (Sorex araneus). Nevertheless, spillover infections of atypical host species were also recorded for KURV, TULV, and another shrew-borne orthohantavirus, Asikkala virus. In addition, indications of virus-specific patterns of tissue tropism were observed. Our results highlight the circulation of several orthohantaviruses, including KURV, which is pathogenic to humans, among rodents and shrews in the Czech Republic.https://journals.asm.org/doi/10.1128/spectrum.01306-22Kurkino virusTula virusSeewis virusAsikkala virusrodentsEulipotyphla |
| spellingShingle | Václav Hönig Jan Kamiš Aneta Maršíková Tereza Matějková Pavel Stopka Anna Mácová Daniel Růžek Jana Kvičerová Orthohantaviruses in Reservoir and Atypical Hosts in the Czech Republic: Spillover Infection and Indication of Virus-Specific Tissue Tropism Microbiology Spectrum Kurkino virus Tula virus Seewis virus Asikkala virus rodents Eulipotyphla |
| title | Orthohantaviruses in Reservoir and Atypical Hosts in the Czech Republic: Spillover Infection and Indication of Virus-Specific Tissue Tropism |
| title_full | Orthohantaviruses in Reservoir and Atypical Hosts in the Czech Republic: Spillover Infection and Indication of Virus-Specific Tissue Tropism |
| title_fullStr | Orthohantaviruses in Reservoir and Atypical Hosts in the Czech Republic: Spillover Infection and Indication of Virus-Specific Tissue Tropism |
| title_full_unstemmed | Orthohantaviruses in Reservoir and Atypical Hosts in the Czech Republic: Spillover Infection and Indication of Virus-Specific Tissue Tropism |
| title_short | Orthohantaviruses in Reservoir and Atypical Hosts in the Czech Republic: Spillover Infection and Indication of Virus-Specific Tissue Tropism |
| title_sort | orthohantaviruses in reservoir and atypical hosts in the czech republic spillover infection and indication of virus specific tissue tropism |
| topic | Kurkino virus Tula virus Seewis virus Asikkala virus rodents Eulipotyphla |
| url | https://journals.asm.org/doi/10.1128/spectrum.01306-22 |
| work_keys_str_mv | AT vaclavhonig orthohantavirusesinreservoirandatypicalhostsintheczechrepublicspilloverinfectionandindicationofvirusspecifictissuetropism AT jankamis orthohantavirusesinreservoirandatypicalhostsintheczechrepublicspilloverinfectionandindicationofvirusspecifictissuetropism AT anetamarsikova orthohantavirusesinreservoirandatypicalhostsintheczechrepublicspilloverinfectionandindicationofvirusspecifictissuetropism AT terezamatejkova orthohantavirusesinreservoirandatypicalhostsintheczechrepublicspilloverinfectionandindicationofvirusspecifictissuetropism AT pavelstopka orthohantavirusesinreservoirandatypicalhostsintheczechrepublicspilloverinfectionandindicationofvirusspecifictissuetropism AT annamacova orthohantavirusesinreservoirandatypicalhostsintheczechrepublicspilloverinfectionandindicationofvirusspecifictissuetropism AT danielruzek orthohantavirusesinreservoirandatypicalhostsintheczechrepublicspilloverinfectionandindicationofvirusspecifictissuetropism AT janakvicerova orthohantavirusesinreservoirandatypicalhostsintheczechrepublicspilloverinfectionandindicationofvirusspecifictissuetropism |