Molecular and biochemical characterisation of Trypanosoma cruzi phosphofructokinase
The characterisation of the gene encoding Trypanosoma cruzi CL Brener phosphofructokinase (PFK) and the biochemical properties of the expressed enzyme are reported here. In contradiction with previous reports, the PFK genes of CL Brener and YBM strain T. cruzi were found to be similar to their Leish...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2009-08-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500014 |
| _version_ | 1797713379000844288 |
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| author | Evelyn Rodríguez Noelia Lander Jose Luis Ramirez |
| author_facet | Evelyn Rodríguez Noelia Lander Jose Luis Ramirez |
| author_sort | Evelyn Rodríguez |
| collection | DOAJ |
| description | The characterisation of the gene encoding Trypanosoma cruzi CL Brener phosphofructokinase (PFK) and the biochemical properties of the expressed enzyme are reported here. In contradiction with previous reports, the PFK genes of CL Brener and YBM strain T. cruzi were found to be similar to their Leishmania mexicana and Trypanosoma brucei homologs in terms of both kinetic properties and size, with open reading frames encoding polypeptides with a deduced molecular mass of 53,483. The predicted amino acid sequence contains the C-terminal glycosome-targeting tripeptide SKL; this localisation was confirmed by immunofluorescence assays. In sequence comparisons with the genes of other eukaryotes, it was found that, despite being an adenosine triphosphate-dependent enzyme, T. cruzi PFK shows significant sequence similarity with inorganic pyrophosphate-dependent PFKs. |
| first_indexed | 2024-03-12T07:35:41Z |
| format | Article |
| id | doaj.art-e050789e2a444789b35cc1b866af3771 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:35:41Z |
| publishDate | 2009-08-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-e050789e2a444789b35cc1b866af37712023-09-02T21:33:21ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602009-08-01104574574810.1590/S0074-02762009000500014Molecular and biochemical characterisation of Trypanosoma cruzi phosphofructokinaseEvelyn RodríguezNoelia LanderJose Luis RamirezThe characterisation of the gene encoding Trypanosoma cruzi CL Brener phosphofructokinase (PFK) and the biochemical properties of the expressed enzyme are reported here. In contradiction with previous reports, the PFK genes of CL Brener and YBM strain T. cruzi were found to be similar to their Leishmania mexicana and Trypanosoma brucei homologs in terms of both kinetic properties and size, with open reading frames encoding polypeptides with a deduced molecular mass of 53,483. The predicted amino acid sequence contains the C-terminal glycosome-targeting tripeptide SKL; this localisation was confirmed by immunofluorescence assays. In sequence comparisons with the genes of other eukaryotes, it was found that, despite being an adenosine triphosphate-dependent enzyme, T. cruzi PFK shows significant sequence similarity with inorganic pyrophosphate-dependent PFKs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500014Trypanosoma cruziKinetoplastidaphosphofructokinaseglycolytic enzymemolecular biochemical characterisation |
| spellingShingle | Evelyn Rodríguez Noelia Lander Jose Luis Ramirez Molecular and biochemical characterisation of Trypanosoma cruzi phosphofructokinase Memorias do Instituto Oswaldo Cruz Trypanosoma cruzi Kinetoplastida phosphofructokinase glycolytic enzyme molecular biochemical characterisation |
| title | Molecular and biochemical characterisation of Trypanosoma cruzi phosphofructokinase |
| title_full | Molecular and biochemical characterisation of Trypanosoma cruzi phosphofructokinase |
| title_fullStr | Molecular and biochemical characterisation of Trypanosoma cruzi phosphofructokinase |
| title_full_unstemmed | Molecular and biochemical characterisation of Trypanosoma cruzi phosphofructokinase |
| title_short | Molecular and biochemical characterisation of Trypanosoma cruzi phosphofructokinase |
| title_sort | molecular and biochemical characterisation of trypanosoma cruzi phosphofructokinase |
| topic | Trypanosoma cruzi Kinetoplastida phosphofructokinase glycolytic enzyme molecular biochemical characterisation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500014 |
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