Intravital imaging of the functions of immune cells in the tumor microenvironment during immunotherapy
Cancer immunotherapy has developed rapidly in recent years and stands as one of the most promising techniques for combating cancer. To develop and optimize cancer immunotherapy, it is crucial to comprehend the interactions between immune cells and tumor cells in the tumor microenvironment (TME). The...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1288273/full |
| _version_ | 1797403394568093696 |
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| author | Xuwen Peng Xuwen Peng Yuke Wang Yuke Wang Jie Zhang Jie Zhang Zhihong Zhang Zhihong Zhang Shuhong Qi Shuhong Qi |
| author_facet | Xuwen Peng Xuwen Peng Yuke Wang Yuke Wang Jie Zhang Jie Zhang Zhihong Zhang Zhihong Zhang Shuhong Qi Shuhong Qi |
| author_sort | Xuwen Peng |
| collection | DOAJ |
| description | Cancer immunotherapy has developed rapidly in recent years and stands as one of the most promising techniques for combating cancer. To develop and optimize cancer immunotherapy, it is crucial to comprehend the interactions between immune cells and tumor cells in the tumor microenvironment (TME). The TME is complex, with the distribution and function of immune cells undergoing dynamic changes. There are several research techniques to study the TME, and intravital imaging emerges as a powerful tool for capturing the spatiotemporal dynamics, especially the movement behavior and the immune function of various immune cells in real physiological state. Intravital imaging has several advantages, such as high spatio-temporal resolution, multicolor, dynamic and 4D detection, making it an invaluable tool for visualizing the dynamic processes in the TME. This review summarizes the workflow for intravital imaging technology, multi-color labeling methods, optical imaging windows, methods of imaging data analysis and the latest research in visualizing the spatio-temporal dynamics and function of immune cells in the TME. It is essential to investigate the role played by immune cells in the tumor immune response through intravital imaging. The review deepens our understanding of the unique contribution of intravital imaging to improve the efficiency of cancer immunotherapy. |
| first_indexed | 2024-03-09T02:37:56Z |
| format | Article |
| id | doaj.art-e053f071ef7c41018013bd665ab7125e |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-09T02:37:56Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-e053f071ef7c41018013bd665ab7125e2023-12-06T08:10:22ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-12-011410.3389/fimmu.2023.12882731288273Intravital imaging of the functions of immune cells in the tumor microenvironment during immunotherapyXuwen Peng0Xuwen Peng1Yuke Wang2Yuke Wang3Jie Zhang4Jie Zhang5Zhihong Zhang6Zhihong Zhang7Shuhong Qi8Shuhong Qi9Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei, ChinaMoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei, ChinaMoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei, ChinaMoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei, ChinaMoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei, ChinaMoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaCancer immunotherapy has developed rapidly in recent years and stands as one of the most promising techniques for combating cancer. To develop and optimize cancer immunotherapy, it is crucial to comprehend the interactions between immune cells and tumor cells in the tumor microenvironment (TME). The TME is complex, with the distribution and function of immune cells undergoing dynamic changes. There are several research techniques to study the TME, and intravital imaging emerges as a powerful tool for capturing the spatiotemporal dynamics, especially the movement behavior and the immune function of various immune cells in real physiological state. Intravital imaging has several advantages, such as high spatio-temporal resolution, multicolor, dynamic and 4D detection, making it an invaluable tool for visualizing the dynamic processes in the TME. This review summarizes the workflow for intravital imaging technology, multi-color labeling methods, optical imaging windows, methods of imaging data analysis and the latest research in visualizing the spatio-temporal dynamics and function of immune cells in the TME. It is essential to investigate the role played by immune cells in the tumor immune response through intravital imaging. The review deepens our understanding of the unique contribution of intravital imaging to improve the efficiency of cancer immunotherapy.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1288273/fullintravital imagingtumor microenvironmentimmune cellsspatio-temporal informationmovement behaviorcancer immunotherapy |
| spellingShingle | Xuwen Peng Xuwen Peng Yuke Wang Yuke Wang Jie Zhang Jie Zhang Zhihong Zhang Zhihong Zhang Shuhong Qi Shuhong Qi Intravital imaging of the functions of immune cells in the tumor microenvironment during immunotherapy Frontiers in Immunology intravital imaging tumor microenvironment immune cells spatio-temporal information movement behavior cancer immunotherapy |
| title | Intravital imaging of the functions of immune cells in the tumor microenvironment during immunotherapy |
| title_full | Intravital imaging of the functions of immune cells in the tumor microenvironment during immunotherapy |
| title_fullStr | Intravital imaging of the functions of immune cells in the tumor microenvironment during immunotherapy |
| title_full_unstemmed | Intravital imaging of the functions of immune cells in the tumor microenvironment during immunotherapy |
| title_short | Intravital imaging of the functions of immune cells in the tumor microenvironment during immunotherapy |
| title_sort | intravital imaging of the functions of immune cells in the tumor microenvironment during immunotherapy |
| topic | intravital imaging tumor microenvironment immune cells spatio-temporal information movement behavior cancer immunotherapy |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1288273/full |
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