Chemical Composition, Antibacterial Activity and <i>In Vitro</i> Anticancer Evaluation of <i>Ochradenus baccatus</i> Methanolic Extract
<i>Background and Objectives: Ochradenus baccatus</i> belongs to the family Resedaceae. It is widely spread in Saudi Arabia and other countries in Southwest Asia. <i>O. baccatus</i> is extensively used in traditional medicine as an anti-inflammatory and antibacterial agent, i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/59/3/546 |
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| author | Weam M. A. Khojali Weiam Hussein Mohammed Khaled Bin Break Ahmed Alafnan Bader Huwaimel Nasrin E. Khalifa Wafa F. S. Badulla Raghad Abdulkarem Alshammari Lama Khalid Alshammari Rehab Aladham Raji Alshammari Sara Mohsen Albarak Enas Hmdan Alrkad Tooba Mahboob Hisham Alshammari |
| author_facet | Weam M. A. Khojali Weiam Hussein Mohammed Khaled Bin Break Ahmed Alafnan Bader Huwaimel Nasrin E. Khalifa Wafa F. S. Badulla Raghad Abdulkarem Alshammari Lama Khalid Alshammari Rehab Aladham Raji Alshammari Sara Mohsen Albarak Enas Hmdan Alrkad Tooba Mahboob Hisham Alshammari |
| author_sort | Weam M. A. Khojali |
| collection | DOAJ |
| description | <i>Background and Objectives: Ochradenus baccatus</i> belongs to the family Resedaceae. It is widely spread in Saudi Arabia and other countries in Southwest Asia. <i>O. baccatus</i> is extensively used in traditional medicine as an anti-inflammatory and antibacterial agent, in addition to being a vital source of food for certain desert animal species. The aim of the present study was to investigate the chemical composition and antibacterial/anticancer activities of <i>O. baccatus</i> methanolic extracts collected from Hail, Saudi Arabia. <i>Materials and Methods:</i> The <i>O. baccatus</i> extracts were obtained by macerating the crude powder in methanol, followed by filtration and evaporation. Liquid chromatography–mass spectrometry (<i>LC-MS</i>) was used to analyze the methanolic extracts’ chemical constituents. Broth microdilution assay for minimum inhibitory concentration (MIC) determination was used to assess antimicrobial activity, while the extracts’ anticancer potential was assessed by sulforhodamine B Assay (SRB) assay. <i>Results:</i> The results of the antibacterial assay showed that the methanolic extracts from the roots and branches possessed varying degrees of activity against particular bacterial strains, with the highest activity being exerted by the branches’ extract against <i>Escherichia coli</i> and <i>Salmonella typhimurium</i> (St), demonstrating MIC values of 15.6 µg/mL and 20 µg/mL, respectively. Furthermore, the SRB cell viability assay revealed that only the branches’ extract inhibited the growth of A549 cancer cells, with an IC<sub>50</sub> value of 86.19 µg/mL. The <i>LC-MS</i> analysis of the methanolic extracts from the plant’s roots and branches was then conducted, resulting in the identification of 8 and 13 major chemical constituents, respectively. Azelaic acid, β-amyrin, and phytanic acid are some of the bioactive compounds that were detected in the extracts via <i>LC-MS</i>, and they are thought to be responsible for the observed antibacterial/anticancer activity of <i>O. baccatus</i> methanolic extracts. <i>Conclusions:</i> This study confirmed the antibacterial/anticancer potential of <i>O. baccatus</i> methanolic extracts and analyzed their phytochemical constituents. Further isolation and biological screening are warranted to understand the therapeutic potential of <i>O. baccatus</i>. |
| first_indexed | 2024-03-11T06:12:49Z |
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| institution | Directory Open Access Journal |
| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-03-11T06:12:49Z |
| publishDate | 2023-03-01 |
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| record_format | Article |
| series | Medicina |
| spelling | doaj.art-e055ee2d74f644cfaaa76e20a83ac61b2023-11-17T12:32:11ZengMDPI AGMedicina1010-660X1648-91442023-03-0159354610.3390/medicina59030546Chemical Composition, Antibacterial Activity and <i>In Vitro</i> Anticancer Evaluation of <i>Ochradenus baccatus</i> Methanolic ExtractWeam M. A. Khojali0Weiam Hussein1Mohammed Khaled Bin Break2Ahmed Alafnan3Bader Huwaimel4Nasrin E. Khalifa5Wafa F. S. Badulla6Raghad Abdulkarem Alshammari7Lama Khalid Alshammari8Rehab Aladham Raji Alshammari9Sara Mohsen Albarak10Enas Hmdan Alrkad11Tooba Mahboob12Hisham Alshammari13Department of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail 2240, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail 2240, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail 2240, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, University of Hail, Hail 2240, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail 2240, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, University of Hail, Hail 2240, Saudi ArabiaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Aden University, Aden 6075, YemenDepartment of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail 2240, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail 2240, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail 2240, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail 2240, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail 2240, Saudi ArabiaFaculty of Pharmaceutical Sciences, UCSI University, KL Campus, Kuala Lumpur 56000, MalaysiaDepartment of Clinical Pharmacy, College of Pharmacy, University of Hail, Hail 2240, Saudi Arabia<i>Background and Objectives: Ochradenus baccatus</i> belongs to the family Resedaceae. It is widely spread in Saudi Arabia and other countries in Southwest Asia. <i>O. baccatus</i> is extensively used in traditional medicine as an anti-inflammatory and antibacterial agent, in addition to being a vital source of food for certain desert animal species. The aim of the present study was to investigate the chemical composition and antibacterial/anticancer activities of <i>O. baccatus</i> methanolic extracts collected from Hail, Saudi Arabia. <i>Materials and Methods:</i> The <i>O. baccatus</i> extracts were obtained by macerating the crude powder in methanol, followed by filtration and evaporation. Liquid chromatography–mass spectrometry (<i>LC-MS</i>) was used to analyze the methanolic extracts’ chemical constituents. Broth microdilution assay for minimum inhibitory concentration (MIC) determination was used to assess antimicrobial activity, while the extracts’ anticancer potential was assessed by sulforhodamine B Assay (SRB) assay. <i>Results:</i> The results of the antibacterial assay showed that the methanolic extracts from the roots and branches possessed varying degrees of activity against particular bacterial strains, with the highest activity being exerted by the branches’ extract against <i>Escherichia coli</i> and <i>Salmonella typhimurium</i> (St), demonstrating MIC values of 15.6 µg/mL and 20 µg/mL, respectively. Furthermore, the SRB cell viability assay revealed that only the branches’ extract inhibited the growth of A549 cancer cells, with an IC<sub>50</sub> value of 86.19 µg/mL. The <i>LC-MS</i> analysis of the methanolic extracts from the plant’s roots and branches was then conducted, resulting in the identification of 8 and 13 major chemical constituents, respectively. Azelaic acid, β-amyrin, and phytanic acid are some of the bioactive compounds that were detected in the extracts via <i>LC-MS</i>, and they are thought to be responsible for the observed antibacterial/anticancer activity of <i>O. baccatus</i> methanolic extracts. <i>Conclusions:</i> This study confirmed the antibacterial/anticancer potential of <i>O. baccatus</i> methanolic extracts and analyzed their phytochemical constituents. Further isolation and biological screening are warranted to understand the therapeutic potential of <i>O. baccatus</i>.https://www.mdpi.com/1648-9144/59/3/546<i>Ochradenus baccatus</i>phytochemicalsantibacterialcytotoxicityHail |
| spellingShingle | Weam M. A. Khojali Weiam Hussein Mohammed Khaled Bin Break Ahmed Alafnan Bader Huwaimel Nasrin E. Khalifa Wafa F. S. Badulla Raghad Abdulkarem Alshammari Lama Khalid Alshammari Rehab Aladham Raji Alshammari Sara Mohsen Albarak Enas Hmdan Alrkad Tooba Mahboob Hisham Alshammari Chemical Composition, Antibacterial Activity and <i>In Vitro</i> Anticancer Evaluation of <i>Ochradenus baccatus</i> Methanolic Extract Medicina <i>Ochradenus baccatus</i> phytochemicals antibacterial cytotoxicity Hail |
| title | Chemical Composition, Antibacterial Activity and <i>In Vitro</i> Anticancer Evaluation of <i>Ochradenus baccatus</i> Methanolic Extract |
| title_full | Chemical Composition, Antibacterial Activity and <i>In Vitro</i> Anticancer Evaluation of <i>Ochradenus baccatus</i> Methanolic Extract |
| title_fullStr | Chemical Composition, Antibacterial Activity and <i>In Vitro</i> Anticancer Evaluation of <i>Ochradenus baccatus</i> Methanolic Extract |
| title_full_unstemmed | Chemical Composition, Antibacterial Activity and <i>In Vitro</i> Anticancer Evaluation of <i>Ochradenus baccatus</i> Methanolic Extract |
| title_short | Chemical Composition, Antibacterial Activity and <i>In Vitro</i> Anticancer Evaluation of <i>Ochradenus baccatus</i> Methanolic Extract |
| title_sort | chemical composition antibacterial activity and i in vitro i anticancer evaluation of i ochradenus baccatus i methanolic extract |
| topic | <i>Ochradenus baccatus</i> phytochemicals antibacterial cytotoxicity Hail |
| url | https://www.mdpi.com/1648-9144/59/3/546 |
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