Lactoferrin Alleviates Ethanol-Induced Injury via Promoting Nrf2 Nuclear Translocation in BRL-3A Rat Liver Cells
Our previous animal studies found that the preventive effects of lactoferrin (Lf) on alcoholic liver injury (ALI) are associated with nuclear factor E2-related factor 2 (Nrf2). To further explore the causality, experiments were performed using rat normal liver BRL-3A cells. Lf treatment reduced etha...
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/23/16848 |
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| author | Deming Li Li Ding Yilin Yan Yifei Xing Jiaying Xu Liqiang Qin |
| author_facet | Deming Li Li Ding Yilin Yan Yifei Xing Jiaying Xu Liqiang Qin |
| author_sort | Deming Li |
| collection | DOAJ |
| description | Our previous animal studies found that the preventive effects of lactoferrin (Lf) on alcoholic liver injury (ALI) are associated with nuclear factor E2-related factor 2 (Nrf2). To further explore the causality, experiments were performed using rat normal liver BRL-3A cells. Lf treatment reduced ethanol-induced death and apoptosis; meanwhile, Lf treatment alleviated excessive LDH release. These findings confirmed the protection of Lf against ethanol-induced injury in BRL-3A cells. Mechanistically, Lf treatment reversed the reduction in nuclear Nrf2 induced by ethanol without affecting the cytoplasmic Nrf2 level, which led to antioxidant enzyme activity restoration. However, the blocking of Nrf2 nuclear translocation by ML385 eliminated the protective effects of Lf. In a conclusion, Lf protects BRL-3A cells from ethanol-induced injury via promoting Nrf2 nuclear translocation. |
| first_indexed | 2024-03-09T01:50:19Z |
| format | Article |
| id | doaj.art-e058273481d74cee9837b552552c6410 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T01:50:19Z |
| publishDate | 2023-11-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e058273481d74cee9837b552552c64102023-12-08T15:17:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124231684810.3390/ijms242316848Lactoferrin Alleviates Ethanol-Induced Injury via Promoting Nrf2 Nuclear Translocation in BRL-3A Rat Liver CellsDeming Li0Li Ding1Yilin Yan2Yifei Xing3Jiaying Xu4Liqiang Qin5School of Public Health, Suzhou Medical College of Soochow University, 199 Renai Road, Suzhou 215123, ChinaSchool of Public Health, Suzhou Medical College of Soochow University, 199 Renai Road, Suzhou 215123, ChinaSchool of Public Health, Suzhou Medical College of Soochow University, 199 Renai Road, Suzhou 215123, ChinaSchool of Public Health, Suzhou Medical College of Soochow University, 199 Renai Road, Suzhou 215123, ChinaState Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Suzhou Medical College of Soochow University, 199 Renai Road, Suzhou 215123, ChinaSchool of Public Health, Suzhou Medical College of Soochow University, 199 Renai Road, Suzhou 215123, ChinaOur previous animal studies found that the preventive effects of lactoferrin (Lf) on alcoholic liver injury (ALI) are associated with nuclear factor E2-related factor 2 (Nrf2). To further explore the causality, experiments were performed using rat normal liver BRL-3A cells. Lf treatment reduced ethanol-induced death and apoptosis; meanwhile, Lf treatment alleviated excessive LDH release. These findings confirmed the protection of Lf against ethanol-induced injury in BRL-3A cells. Mechanistically, Lf treatment reversed the reduction in nuclear Nrf2 induced by ethanol without affecting the cytoplasmic Nrf2 level, which led to antioxidant enzyme activity restoration. However, the blocking of Nrf2 nuclear translocation by ML385 eliminated the protective effects of Lf. In a conclusion, Lf protects BRL-3A cells from ethanol-induced injury via promoting Nrf2 nuclear translocation.https://www.mdpi.com/1422-0067/24/23/16848lactoferrinethanol-induced injuryalleviatesNrf2BRL-3A rat liver cell line |
| spellingShingle | Deming Li Li Ding Yilin Yan Yifei Xing Jiaying Xu Liqiang Qin Lactoferrin Alleviates Ethanol-Induced Injury via Promoting Nrf2 Nuclear Translocation in BRL-3A Rat Liver Cells International Journal of Molecular Sciences lactoferrin ethanol-induced injury alleviates Nrf2 BRL-3A rat liver cell line |
| title | Lactoferrin Alleviates Ethanol-Induced Injury via Promoting Nrf2 Nuclear Translocation in BRL-3A Rat Liver Cells |
| title_full | Lactoferrin Alleviates Ethanol-Induced Injury via Promoting Nrf2 Nuclear Translocation in BRL-3A Rat Liver Cells |
| title_fullStr | Lactoferrin Alleviates Ethanol-Induced Injury via Promoting Nrf2 Nuclear Translocation in BRL-3A Rat Liver Cells |
| title_full_unstemmed | Lactoferrin Alleviates Ethanol-Induced Injury via Promoting Nrf2 Nuclear Translocation in BRL-3A Rat Liver Cells |
| title_short | Lactoferrin Alleviates Ethanol-Induced Injury via Promoting Nrf2 Nuclear Translocation in BRL-3A Rat Liver Cells |
| title_sort | lactoferrin alleviates ethanol induced injury via promoting nrf2 nuclear translocation in brl 3a rat liver cells |
| topic | lactoferrin ethanol-induced injury alleviates Nrf2 BRL-3A rat liver cell line |
| url | https://www.mdpi.com/1422-0067/24/23/16848 |
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