Exploration of the interchromosomal effects in preimplantation genetic testing for structural rearrangements based on next‐generation sequencing
Abstract Background To investigate the interchromosomal effect (ICE) in chromosome translocation carriers. Methods Data on preimplantation genetic testing aneuploidy and structural rearrangements (translocation) were retrospectively collected and classified into a reciprocal translocation group, a R...
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| Format: | Article |
| Language: | English |
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Wiley
2022-09-01
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| Series: | Molecular Genetics & Genomic Medicine |
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| Online Access: | https://doi.org/10.1002/mgg3.2017 |
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| author | Junmei Fan Xueluo Zhang Yanhua Chen Junkun Zhang Lei Zhang Xingyu Bi Jinbao Wang Xiang Huang Meiqin Yan Xueqing Wu |
| author_facet | Junmei Fan Xueluo Zhang Yanhua Chen Junkun Zhang Lei Zhang Xingyu Bi Jinbao Wang Xiang Huang Meiqin Yan Xueqing Wu |
| author_sort | Junmei Fan |
| collection | DOAJ |
| description | Abstract Background To investigate the interchromosomal effect (ICE) in chromosome translocation carriers. Methods Data on preimplantation genetic testing aneuploidy and structural rearrangements (translocation) were retrospectively collected and classified into a reciprocal translocation group, a Robertsonian translocation group and a control group. According to the carrier's gender and age, all cases underwent further subgroup difference analysis of de novo abnormal embryo rates and the number of chromosomes involved in de novo abnormal embryos. Results Among the 283 couples who participated in this study, 1076 blastocysts from 352 cycles were collected, and 246 de novo abnormal embryos were included. There was a significant difference in the rate of de novo abnormal embryos among the three groups (p < .05) but no significant difference in the number of de novo abnormal chromosomes in the abnormal embryos (p > .05). Gender and age (classified by 35 years old) had no effect on the de novo abnormal embryo ratios among the translocation carriers (p > .05). However, the de novo abnormal ratio increased with age. The embryo constitution reflected no significant difference between the translocation groups (p > .05). Conclusion The ICE was detected for the translocation carriers. The de novo abnormal embryo ratio increased with age. Gender had no effect on the de novo abnormal embryo ratio. Translocation status played a more important role than age and gender. |
| first_indexed | 2024-04-11T20:24:15Z |
| format | Article |
| id | doaj.art-e05842ea0cc2462993474f9de6a9a160 |
| institution | Directory Open Access Journal |
| issn | 2324-9269 |
| language | English |
| last_indexed | 2024-04-11T20:24:15Z |
| publishDate | 2022-09-01 |
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| series | Molecular Genetics & Genomic Medicine |
| spelling | doaj.art-e05842ea0cc2462993474f9de6a9a1602022-12-22T04:04:43ZengWileyMolecular Genetics & Genomic Medicine2324-92692022-09-01109n/an/a10.1002/mgg3.2017Exploration of the interchromosomal effects in preimplantation genetic testing for structural rearrangements based on next‐generation sequencingJunmei Fan0Xueluo Zhang1Yanhua Chen2Junkun Zhang3Lei Zhang4Xingyu Bi5Jinbao Wang6Xiang Huang7Meiqin Yan8Xueqing Wu9Department of Reproductive Medicine Center, Children's Hospital of Shanxi and Women Health Center of Shanxi Affiliated of Shanxi Medical University Taiyuan ChinaDepartment of Reproductive Medicine Center, Children's Hospital of Shanxi and Women Health Center of Shanxi Affiliated of Shanxi Medical University Taiyuan ChinaDepartment of Reproductive Medicine Center, Children's Hospital of Shanxi and Women Health Center of Shanxi Affiliated of Shanxi Medical University Taiyuan ChinaDepartment of Medical College Datong University of Shanxi Datong ChinaDepartment of Reproductive Medicine Center, Children's Hospital of Shanxi and Women Health Center of Shanxi Affiliated of Shanxi Medical University Taiyuan ChinaDepartment of Reproductive Medicine Center, Children's Hospital of Shanxi and Women Health Center of Shanxi Affiliated of Shanxi Medical University Taiyuan ChinaDepartment of Reproductive Medicine Center, Children's Hospital of Shanxi and Women Health Center of Shanxi Affiliated of Shanxi Medical University Taiyuan ChinaDepartment of Reproductive Medicine Center, Children's Hospital of Shanxi and Women Health Center of Shanxi Affiliated of Shanxi Medical University Taiyuan ChinaDepartment of Science and Education Division Children's Hospital of Shanxi and Women Health Center of Shanxi Taiyuan ChinaDepartment of Reproductive Medicine Center, Children's Hospital of Shanxi and Women Health Center of Shanxi Affiliated of Shanxi Medical University Taiyuan ChinaAbstract Background To investigate the interchromosomal effect (ICE) in chromosome translocation carriers. Methods Data on preimplantation genetic testing aneuploidy and structural rearrangements (translocation) were retrospectively collected and classified into a reciprocal translocation group, a Robertsonian translocation group and a control group. According to the carrier's gender and age, all cases underwent further subgroup difference analysis of de novo abnormal embryo rates and the number of chromosomes involved in de novo abnormal embryos. Results Among the 283 couples who participated in this study, 1076 blastocysts from 352 cycles were collected, and 246 de novo abnormal embryos were included. There was a significant difference in the rate of de novo abnormal embryos among the three groups (p < .05) but no significant difference in the number of de novo abnormal chromosomes in the abnormal embryos (p > .05). Gender and age (classified by 35 years old) had no effect on the de novo abnormal embryo ratios among the translocation carriers (p > .05). However, the de novo abnormal ratio increased with age. The embryo constitution reflected no significant difference between the translocation groups (p > .05). Conclusion The ICE was detected for the translocation carriers. The de novo abnormal embryo ratio increased with age. Gender had no effect on the de novo abnormal embryo ratio. Translocation status played a more important role than age and gender.https://doi.org/10.1002/mgg3.2017agegenderinterchromosomal effectnext‐generation sequencingpreimplantation genetic testingtranslocation |
| spellingShingle | Junmei Fan Xueluo Zhang Yanhua Chen Junkun Zhang Lei Zhang Xingyu Bi Jinbao Wang Xiang Huang Meiqin Yan Xueqing Wu Exploration of the interchromosomal effects in preimplantation genetic testing for structural rearrangements based on next‐generation sequencing Molecular Genetics & Genomic Medicine age gender interchromosomal effect next‐generation sequencing preimplantation genetic testing translocation |
| title | Exploration of the interchromosomal effects in preimplantation genetic testing for structural rearrangements based on next‐generation sequencing |
| title_full | Exploration of the interchromosomal effects in preimplantation genetic testing for structural rearrangements based on next‐generation sequencing |
| title_fullStr | Exploration of the interchromosomal effects in preimplantation genetic testing for structural rearrangements based on next‐generation sequencing |
| title_full_unstemmed | Exploration of the interchromosomal effects in preimplantation genetic testing for structural rearrangements based on next‐generation sequencing |
| title_short | Exploration of the interchromosomal effects in preimplantation genetic testing for structural rearrangements based on next‐generation sequencing |
| title_sort | exploration of the interchromosomal effects in preimplantation genetic testing for structural rearrangements based on next generation sequencing |
| topic | age gender interchromosomal effect next‐generation sequencing preimplantation genetic testing translocation |
| url | https://doi.org/10.1002/mgg3.2017 |
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