Dynamic Expression of the Homeobox Factor PBX1 during Mouse Testis Development
Members of the pre-B-cell leukemia transcription factor (PBX) family of homeoproteins are mainly known for their involvement in hematopoietic cell differentiation and in the development of leukemia. The four PBX proteins, PBX1, PBX2, PBX3 and PBX4, belong to the three amino acid loop extension (TALE...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Endocrines |
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| Online Access: | https://www.mdpi.com/2673-396X/3/1/2 |
| _version_ | 1797471895761715200 |
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| author | Vanessa Moisan Catherine Brousseau Jacques J. Tremblay |
| author_facet | Vanessa Moisan Catherine Brousseau Jacques J. Tremblay |
| author_sort | Vanessa Moisan |
| collection | DOAJ |
| description | Members of the pre-B-cell leukemia transcription factor (PBX) family of homeoproteins are mainly known for their involvement in hematopoietic cell differentiation and in the development of leukemia. The four PBX proteins, PBX1, PBX2, PBX3 and PBX4, belong to the three amino acid loop extension (TALE) superfamily of homeoproteins which are important transcriptional cofactors in several developmental processes involving homeobox (HOX) factors. Mutations in the human <i>PBX1</i> gene are responsible for cases of gonadal dysgenesis with absence of male sex differentiation while <i>Pbx1</i> inactivation in the mouse causes a failure in Leydig cell differentiation and function. However, no data is available regarding the expression profile of this transcription factor in the testis. To fill this knowledge gap, we have characterized PBX1 expression during mouse testicular development. Real time PCRs and Western blots confirmed the presence <i>Pbx1</i> mRNA and PBX1 protein in different Leydig and Sertoli cell lines. The cellular localization of the PBX1 protein was determined by immunohistochemistry and immunofluorescence on mouse testis sections at different embryonic and postnatal developmental stages. PBX1 was detected in interstitial cells and in peritubular myoid cells from embryonic life until puberty. Most interstitial cells expressing PBX1 do not express the Leydig cell marker CYP17A1, indicating that they are not differentiated and steroidogenically active Leydig cells. In adults, PBX1 was mainly detected in Sertoli cells. The presence of PBX1 in different somatic cell populations during testicular development further supports a direct role for this transcription factor in testis cell differentiation and in male reproductive function. |
| first_indexed | 2024-03-09T19:54:33Z |
| format | Article |
| id | doaj.art-e0588305bfdc45a790e4ae584edf81f0 |
| institution | Directory Open Access Journal |
| issn | 2673-396X |
| language | English |
| last_indexed | 2024-03-09T19:54:33Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Endocrines |
| spelling | doaj.art-e0588305bfdc45a790e4ae584edf81f02023-11-24T01:01:58ZengMDPI AGEndocrines2673-396X2022-01-0131162810.3390/endocrines3010002Dynamic Expression of the Homeobox Factor PBX1 during Mouse Testis DevelopmentVanessa Moisan0Catherine Brousseau1Jacques J. Tremblay2Reproduction, Mother and Child Health, Room T3-67, CHU de Québec-Université Laval Research Centre, 2705 Laurier Blvd., Québec, QC G1V 4G2, CanadaReproduction, Mother and Child Health, Room T3-67, CHU de Québec-Université Laval Research Centre, 2705 Laurier Blvd., Québec, QC G1V 4G2, CanadaReproduction, Mother and Child Health, Room T3-67, CHU de Québec-Université Laval Research Centre, 2705 Laurier Blvd., Québec, QC G1V 4G2, CanadaMembers of the pre-B-cell leukemia transcription factor (PBX) family of homeoproteins are mainly known for their involvement in hematopoietic cell differentiation and in the development of leukemia. The four PBX proteins, PBX1, PBX2, PBX3 and PBX4, belong to the three amino acid loop extension (TALE) superfamily of homeoproteins which are important transcriptional cofactors in several developmental processes involving homeobox (HOX) factors. Mutations in the human <i>PBX1</i> gene are responsible for cases of gonadal dysgenesis with absence of male sex differentiation while <i>Pbx1</i> inactivation in the mouse causes a failure in Leydig cell differentiation and function. However, no data is available regarding the expression profile of this transcription factor in the testis. To fill this knowledge gap, we have characterized PBX1 expression during mouse testicular development. Real time PCRs and Western blots confirmed the presence <i>Pbx1</i> mRNA and PBX1 protein in different Leydig and Sertoli cell lines. The cellular localization of the PBX1 protein was determined by immunohistochemistry and immunofluorescence on mouse testis sections at different embryonic and postnatal developmental stages. PBX1 was detected in interstitial cells and in peritubular myoid cells from embryonic life until puberty. Most interstitial cells expressing PBX1 do not express the Leydig cell marker CYP17A1, indicating that they are not differentiated and steroidogenically active Leydig cells. In adults, PBX1 was mainly detected in Sertoli cells. The presence of PBX1 in different somatic cell populations during testicular development further supports a direct role for this transcription factor in testis cell differentiation and in male reproductive function.https://www.mdpi.com/2673-396X/3/1/2homeoproteinLeydig cellSertoli celltestiscell differentiation |
| spellingShingle | Vanessa Moisan Catherine Brousseau Jacques J. Tremblay Dynamic Expression of the Homeobox Factor PBX1 during Mouse Testis Development Endocrines homeoprotein Leydig cell Sertoli cell testis cell differentiation |
| title | Dynamic Expression of the Homeobox Factor PBX1 during Mouse Testis Development |
| title_full | Dynamic Expression of the Homeobox Factor PBX1 during Mouse Testis Development |
| title_fullStr | Dynamic Expression of the Homeobox Factor PBX1 during Mouse Testis Development |
| title_full_unstemmed | Dynamic Expression of the Homeobox Factor PBX1 during Mouse Testis Development |
| title_short | Dynamic Expression of the Homeobox Factor PBX1 during Mouse Testis Development |
| title_sort | dynamic expression of the homeobox factor pbx1 during mouse testis development |
| topic | homeoprotein Leydig cell Sertoli cell testis cell differentiation |
| url | https://www.mdpi.com/2673-396X/3/1/2 |
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