Expansion of fibroblast cell sheets using a modified MEEK micrografting technique for wound healing applications
Abstract Cell sheet engineering, a scaffold-free approach to fabricate functional tissue constructs from several cell monolayers, has shown promise in tissue regeneration and wound healing. Unfortunately, these cell sheets are often too small to provide sufficient wound area coverage. In this study,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-21913-x |
| _version_ | 1798044534188277760 |
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| author | Kanokaon Benchaprathanphorn Phongphot Sakulaue Wanwipa Siriwatwechakul Pornprom Muangman Kusuma Chinaroonchai Nantaporn Namviriyachote Kwanchanok Viravaidya-Pasuwat |
| author_facet | Kanokaon Benchaprathanphorn Phongphot Sakulaue Wanwipa Siriwatwechakul Pornprom Muangman Kusuma Chinaroonchai Nantaporn Namviriyachote Kwanchanok Viravaidya-Pasuwat |
| author_sort | Kanokaon Benchaprathanphorn |
| collection | DOAJ |
| description | Abstract Cell sheet engineering, a scaffold-free approach to fabricate functional tissue constructs from several cell monolayers, has shown promise in tissue regeneration and wound healing. Unfortunately, these cell sheets are often too small to provide sufficient wound area coverage. In this study, we describe a process to enlarge cell sheets using MEEK micrografting, a technique extensively used to expand skin autografts for large burn treatments. Human dermal fibroblast cell sheets were placed on MEEK’s prefolded gauze without any use of adhesive, cut along the premarked lines and stretched out at various expansion ratios (1:3, 1:6 and 1:9), resulting in regular distribution of many square islands of fibroblasts at a much larger surface area. The cellular processes essential for wound healing, including reattachment, proliferation, and migration, of the fibroblasts on expanded MEEK gauze were superior to those on nylon dressing which served as a control. The optimal expansion ratio with the highest migration rate was 1:6, possibly due to the activation of chemical signals caused by mechanical stretching and an effective intercellular communication distance. Therefore, the combination of cell sheet engineering with the MEEK micrografting technique could provide high quality cells with a large coverage area, which would be particularly beneficial in wound care applications. |
| first_indexed | 2024-04-11T23:05:45Z |
| format | Article |
| id | doaj.art-e05eba1be7ae41da853c5f75fa5757fe |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T23:05:45Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e05eba1be7ae41da853c5f75fa5757fe2022-12-22T03:58:02ZengNature PortfolioScientific Reports2045-23222022-11-0112111110.1038/s41598-022-21913-xExpansion of fibroblast cell sheets using a modified MEEK micrografting technique for wound healing applicationsKanokaon Benchaprathanphorn0Phongphot Sakulaue1Wanwipa Siriwatwechakul2Pornprom Muangman3Kusuma Chinaroonchai4Nantaporn Namviriyachote5Kwanchanok Viravaidya-Pasuwat6Biological Engineering Program, Faculty of Engineering, King Mongkut’s University of Technology ThonburiSchool of Bio-Chemical Engineering and Technology, Sirindhorn International Institute of Technology, Thammasat UniversitySchool of Bio-Chemical Engineering and Technology, Sirindhorn International Institute of Technology, Thammasat UniversityTrauma Division, Department of Surgery, Faculty of Medicine, Siriraj Hospital, Mahidol UniversityTrauma Division, Department of Surgery, Faculty of Medicine, Siriraj Hospital, Mahidol UniversityTrauma Division, Department of Surgery, Faculty of Medicine, Siriraj Hospital, Mahidol UniversityBiological Engineering Program, Faculty of Engineering, King Mongkut’s University of Technology ThonburiAbstract Cell sheet engineering, a scaffold-free approach to fabricate functional tissue constructs from several cell monolayers, has shown promise in tissue regeneration and wound healing. Unfortunately, these cell sheets are often too small to provide sufficient wound area coverage. In this study, we describe a process to enlarge cell sheets using MEEK micrografting, a technique extensively used to expand skin autografts for large burn treatments. Human dermal fibroblast cell sheets were placed on MEEK’s prefolded gauze without any use of adhesive, cut along the premarked lines and stretched out at various expansion ratios (1:3, 1:6 and 1:9), resulting in regular distribution of many square islands of fibroblasts at a much larger surface area. The cellular processes essential for wound healing, including reattachment, proliferation, and migration, of the fibroblasts on expanded MEEK gauze were superior to those on nylon dressing which served as a control. The optimal expansion ratio with the highest migration rate was 1:6, possibly due to the activation of chemical signals caused by mechanical stretching and an effective intercellular communication distance. Therefore, the combination of cell sheet engineering with the MEEK micrografting technique could provide high quality cells with a large coverage area, which would be particularly beneficial in wound care applications.https://doi.org/10.1038/s41598-022-21913-x |
| spellingShingle | Kanokaon Benchaprathanphorn Phongphot Sakulaue Wanwipa Siriwatwechakul Pornprom Muangman Kusuma Chinaroonchai Nantaporn Namviriyachote Kwanchanok Viravaidya-Pasuwat Expansion of fibroblast cell sheets using a modified MEEK micrografting technique for wound healing applications Scientific Reports |
| title | Expansion of fibroblast cell sheets using a modified MEEK micrografting technique for wound healing applications |
| title_full | Expansion of fibroblast cell sheets using a modified MEEK micrografting technique for wound healing applications |
| title_fullStr | Expansion of fibroblast cell sheets using a modified MEEK micrografting technique for wound healing applications |
| title_full_unstemmed | Expansion of fibroblast cell sheets using a modified MEEK micrografting technique for wound healing applications |
| title_short | Expansion of fibroblast cell sheets using a modified MEEK micrografting technique for wound healing applications |
| title_sort | expansion of fibroblast cell sheets using a modified meek micrografting technique for wound healing applications |
| url | https://doi.org/10.1038/s41598-022-21913-x |
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