Experimental models of autism spectrum disorders on the example of the use of brain organelles
Introduction ASD are heterogeneous pathological conditions characterized by difficulties in establishing social contacts and the manifestation of repetitive behavior. An atypical trajectory of brain maturation, impaired neurogenesis, synaptogenesis, and an imbalance in the excitatory and inhibitory...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2023-03-01
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| Series: | European Psychiatry |
| Online Access: | https://www.cambridge.org/core/product/identifier/S0924933823010349/type/journal_article |
| _version_ | 1797616676207853568 |
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| author | A. Sidenkova |
| author_facet | A. Sidenkova |
| author_sort | A. Sidenkova |
| collection | DOAJ |
| description |
Introduction
ASD are heterogeneous pathological conditions characterized by difficulties in establishing social contacts and the manifestation of repetitive behavior. An atypical trajectory of brain maturation, impaired neurogenesis, synaptogenesis, and an imbalance in the excitatory and inhibitory systems of the CNS form the morphofunctional basis of the ASD.
Objectives
To understand the functioning of this complexly organized system in time and space, a three-dimensional model is needed. The closest in vitro model of the human brain from early embryonic stages to aging is brain organoids. Human brain organoids are self-organizing three-dimensional cell aggregates derived from pluripotent stem cells (hiPSCs)
Methods
Organelles generalize neurogenesis, gliogenesis, synaptogenesis, cell migration and cell differentiation, gyrification of the cerebral cortex, and reflect the connections of brain regions.
Results
The use of telencephalon organelles in the RAS model revealed a deficit in neuronal migration, acceleration and disruption of cell cycle synchronization, aberrant cell proliferation, abundant synaptogenesis, temporary deviations in the development of the cortex, increased branching of neurons, unbalanced inhibitory differentiation of neurons, high activity of ion channels is a consequence of a violation of FOXG1 activity. Organelles generalize neurogenesis, gliogenesis, synaptogenesis, cell migration and cell differentiation, gyrification of the cerebral cortex, and reflect the connections of brain regions.The use of telencephalon organelles in the RAS model revealed a deficit in neuronal migration, acceleration and disruption of cell cycle synchronization, aberrant cell proliferation, abundant synaptogenesis, temporary deviations in the development of the cortex, increased branching of neurons, unbalanced inhibitory differentiation of neurons, high activity of ion channels is a consequence of a violation of FOXG1 activity .
Conclusions
hiPSCs can provide insight into the cellular mechanisms underlying ASD as a neuropsychiatric disorder, providing access to the development of platforms for in vitro drug screening and individualized patient therapy.
Disclosure of Interest
None Declared |
| first_indexed | 2024-03-11T07:43:43Z |
| format | Article |
| id | doaj.art-e06318e5331345c6a26e0d488a0c2994 |
| institution | Directory Open Access Journal |
| issn | 0924-9338 1778-3585 |
| language | English |
| last_indexed | 2024-03-11T07:43:43Z |
| publishDate | 2023-03-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | European Psychiatry |
| spelling | doaj.art-e06318e5331345c6a26e0d488a0c29942023-11-17T05:08:04ZengCambridge University PressEuropean Psychiatry0924-93381778-35852023-03-0166S483S48310.1192/j.eurpsy.2023.1034Experimental models of autism spectrum disorders on the example of the use of brain organellesA. Sidenkova0Psychiatry, Ural State Medical University, Yekaterinburg, Russian Federation Introduction ASD are heterogeneous pathological conditions characterized by difficulties in establishing social contacts and the manifestation of repetitive behavior. An atypical trajectory of brain maturation, impaired neurogenesis, synaptogenesis, and an imbalance in the excitatory and inhibitory systems of the CNS form the morphofunctional basis of the ASD. Objectives To understand the functioning of this complexly organized system in time and space, a three-dimensional model is needed. The closest in vitro model of the human brain from early embryonic stages to aging is brain organoids. Human brain organoids are self-organizing three-dimensional cell aggregates derived from pluripotent stem cells (hiPSCs) Methods Organelles generalize neurogenesis, gliogenesis, synaptogenesis, cell migration and cell differentiation, gyrification of the cerebral cortex, and reflect the connections of brain regions. Results The use of telencephalon organelles in the RAS model revealed a deficit in neuronal migration, acceleration and disruption of cell cycle synchronization, aberrant cell proliferation, abundant synaptogenesis, temporary deviations in the development of the cortex, increased branching of neurons, unbalanced inhibitory differentiation of neurons, high activity of ion channels is a consequence of a violation of FOXG1 activity. Organelles generalize neurogenesis, gliogenesis, synaptogenesis, cell migration and cell differentiation, gyrification of the cerebral cortex, and reflect the connections of brain regions.The use of telencephalon organelles in the RAS model revealed a deficit in neuronal migration, acceleration and disruption of cell cycle synchronization, aberrant cell proliferation, abundant synaptogenesis, temporary deviations in the development of the cortex, increased branching of neurons, unbalanced inhibitory differentiation of neurons, high activity of ion channels is a consequence of a violation of FOXG1 activity . Conclusions hiPSCs can provide insight into the cellular mechanisms underlying ASD as a neuropsychiatric disorder, providing access to the development of platforms for in vitro drug screening and individualized patient therapy. Disclosure of Interest None Declaredhttps://www.cambridge.org/core/product/identifier/S0924933823010349/type/journal_article |
| spellingShingle | A. Sidenkova Experimental models of autism spectrum disorders on the example of the use of brain organelles European Psychiatry |
| title | Experimental models of autism spectrum disorders on the example of the use of brain organelles |
| title_full | Experimental models of autism spectrum disorders on the example of the use of brain organelles |
| title_fullStr | Experimental models of autism spectrum disorders on the example of the use of brain organelles |
| title_full_unstemmed | Experimental models of autism spectrum disorders on the example of the use of brain organelles |
| title_short | Experimental models of autism spectrum disorders on the example of the use of brain organelles |
| title_sort | experimental models of autism spectrum disorders on the example of the use of brain organelles |
| url | https://www.cambridge.org/core/product/identifier/S0924933823010349/type/journal_article |
| work_keys_str_mv | AT asidenkova experimentalmodelsofautismspectrumdisordersontheexampleoftheuseofbrainorganelles |