A Mixture of Topical Forms of Polydeoxyribonucleotide, Vitamin C, and Niacinamide Attenuated Skin Pigmentation and Increased Skin Elasticity by Modulating Nuclear Factor Erythroid 2-like 2
It is well-known that increased oxidative stress caused by ultraviolet B (UV-B) radiation induces melanogenesis and activates metalloproteinases (MMPs), which degrade collagen and elastin fibers, leading to decreased skin elasticity. Various antioxidant agents, such as vitamin C and niacinamide, hav...
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MDPI AG
2022-02-01
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| author | Hyoung Moon Kim Kyung-A Byun Seyeon Oh Jin Young Yang Hyun Jun Park Moon Suk Chung Kuk Hui Son Kyunghee Byun |
| author_facet | Hyoung Moon Kim Kyung-A Byun Seyeon Oh Jin Young Yang Hyun Jun Park Moon Suk Chung Kuk Hui Son Kyunghee Byun |
| author_sort | Hyoung Moon Kim |
| collection | DOAJ |
| description | It is well-known that increased oxidative stress caused by ultraviolet B (UV-B) radiation induces melanogenesis and activates metalloproteinases (MMPs), which degrade collagen and elastin fibers, leading to decreased skin elasticity. Various antioxidant agents, such as vitamin C and niacinamide, have been evaluated for use as treatments for photoaging or skin pigmentation. In this study, we evaluated the ability of a topical liquid formula of polydeoxyribonucleotide (PDRN), vitamin C, and niacinamide (PVN) delivered via a microneedling therapy system (MTS) to attenuate photoaging and pigmentation by increasing nuclear factor erythroid 2-like 2 (NRF2)/heme oxygenase-1 (HO-1) and decreasing MMP expression in a UV-B-radiated animal model. The effects of the PVN were compared with those of individual PDRN and hydroquinone (HQ) compounds. The expression of NRF2/HO-1 significantly increased in response to HQ, PDRN, and PVN in UV-B-radiated animal skin. The activity of nicotinamide adenine dinucleotide phosphate hydrogen oxidase decreased in response to HQ, PDRN, and PVN, and the superoxide dismutase activity increased. The expression of tumor protein p53 and microphthalmia-associated transcription factor and tyrosinase activity decreased in response to HQ, PDRN, and PVN, and this decrease was accompanied by decreased melanin content in the skin. The expression of nuclear factor kappa-light-chain enhancer of activated B cells and MMP2/3/9 decreased in response to HQ, PDRN, and PVN in UV-B-radiated skin. However, the expression of collagen type I α1 chain and the amount of collagen fibers that were evaluated by Masson’s trichrome staining increased in response to HQ, PDRN, and PVN. The contents of elastin fibers, fibrillin 1/2 and fibulin 5 increased in response to HQ, PDRN, and PVN. In conclusion, PVN delivered via MTS led to decreased melanogenesis and destruction of collagen and elastin fibers by MMPs, and, thus, PVN decreased skin pigmentation and increased skin elasticity. |
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| language | English |
| last_indexed | 2024-03-09T21:21:14Z |
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| spelling | doaj.art-e067785e419442218e28dac85985f3672023-11-23T21:21:31ZengMDPI AGMolecules1420-30492022-02-01274127610.3390/molecules27041276A Mixture of Topical Forms of Polydeoxyribonucleotide, Vitamin C, and Niacinamide Attenuated Skin Pigmentation and Increased Skin Elasticity by Modulating Nuclear Factor Erythroid 2-like 2Hyoung Moon Kim0Kyung-A Byun1Seyeon Oh2Jin Young Yang3Hyun Jun Park4Moon Suk Chung5Kuk Hui Son6Kyunghee Byun7Department of Anatomy & Cell Biology, College of Medicine, Gachon University, Incheon 21936, KoreaDepartment of Anatomy & Cell Biology, College of Medicine, Gachon University, Incheon 21936, KoreaFunctional Cellular Networks Laboratory, Graduate School and Lee Gil Ya Cancer and Diabetes Institute, College of Medicine, Gachon University, Incheon 21999, KoreaFunctional Cellular Networks Laboratory, Graduate School and Lee Gil Ya Cancer and Diabetes Institute, College of Medicine, Gachon University, Incheon 21999, KoreaMaylin Anti-Aging Center Apgujeong, Seoul 06005, KoreaI’ll Global Co., Inc., Seoul 06532, KoreaDepartment of Thoracic and Cardiovascular Surgery, Gil Medical Center, Gachon University, Incheon 21565, KoreaDepartment of Anatomy & Cell Biology, College of Medicine, Gachon University, Incheon 21936, KoreaIt is well-known that increased oxidative stress caused by ultraviolet B (UV-B) radiation induces melanogenesis and activates metalloproteinases (MMPs), which degrade collagen and elastin fibers, leading to decreased skin elasticity. Various antioxidant agents, such as vitamin C and niacinamide, have been evaluated for use as treatments for photoaging or skin pigmentation. In this study, we evaluated the ability of a topical liquid formula of polydeoxyribonucleotide (PDRN), vitamin C, and niacinamide (PVN) delivered via a microneedling therapy system (MTS) to attenuate photoaging and pigmentation by increasing nuclear factor erythroid 2-like 2 (NRF2)/heme oxygenase-1 (HO-1) and decreasing MMP expression in a UV-B-radiated animal model. The effects of the PVN were compared with those of individual PDRN and hydroquinone (HQ) compounds. The expression of NRF2/HO-1 significantly increased in response to HQ, PDRN, and PVN in UV-B-radiated animal skin. The activity of nicotinamide adenine dinucleotide phosphate hydrogen oxidase decreased in response to HQ, PDRN, and PVN, and the superoxide dismutase activity increased. The expression of tumor protein p53 and microphthalmia-associated transcription factor and tyrosinase activity decreased in response to HQ, PDRN, and PVN, and this decrease was accompanied by decreased melanin content in the skin. The expression of nuclear factor kappa-light-chain enhancer of activated B cells and MMP2/3/9 decreased in response to HQ, PDRN, and PVN in UV-B-radiated skin. However, the expression of collagen type I α1 chain and the amount of collagen fibers that were evaluated by Masson’s trichrome staining increased in response to HQ, PDRN, and PVN. The contents of elastin fibers, fibrillin 1/2 and fibulin 5 increased in response to HQ, PDRN, and PVN. In conclusion, PVN delivered via MTS led to decreased melanogenesis and destruction of collagen and elastin fibers by MMPs, and, thus, PVN decreased skin pigmentation and increased skin elasticity.https://www.mdpi.com/1420-3049/27/4/1276niacinamidenuclear factor erythroid-2-related factor 2polydeoxyribonucleotideskin pigmentationskin pigmentationvitamin C |
| spellingShingle | Hyoung Moon Kim Kyung-A Byun Seyeon Oh Jin Young Yang Hyun Jun Park Moon Suk Chung Kuk Hui Son Kyunghee Byun A Mixture of Topical Forms of Polydeoxyribonucleotide, Vitamin C, and Niacinamide Attenuated Skin Pigmentation and Increased Skin Elasticity by Modulating Nuclear Factor Erythroid 2-like 2 Molecules niacinamide nuclear factor erythroid-2-related factor 2 polydeoxyribonucleotide skin pigmentation skin pigmentation vitamin C |
| title | A Mixture of Topical Forms of Polydeoxyribonucleotide, Vitamin C, and Niacinamide Attenuated Skin Pigmentation and Increased Skin Elasticity by Modulating Nuclear Factor Erythroid 2-like 2 |
| title_full | A Mixture of Topical Forms of Polydeoxyribonucleotide, Vitamin C, and Niacinamide Attenuated Skin Pigmentation and Increased Skin Elasticity by Modulating Nuclear Factor Erythroid 2-like 2 |
| title_fullStr | A Mixture of Topical Forms of Polydeoxyribonucleotide, Vitamin C, and Niacinamide Attenuated Skin Pigmentation and Increased Skin Elasticity by Modulating Nuclear Factor Erythroid 2-like 2 |
| title_full_unstemmed | A Mixture of Topical Forms of Polydeoxyribonucleotide, Vitamin C, and Niacinamide Attenuated Skin Pigmentation and Increased Skin Elasticity by Modulating Nuclear Factor Erythroid 2-like 2 |
| title_short | A Mixture of Topical Forms of Polydeoxyribonucleotide, Vitamin C, and Niacinamide Attenuated Skin Pigmentation and Increased Skin Elasticity by Modulating Nuclear Factor Erythroid 2-like 2 |
| title_sort | mixture of topical forms of polydeoxyribonucleotide vitamin c and niacinamide attenuated skin pigmentation and increased skin elasticity by modulating nuclear factor erythroid 2 like 2 |
| topic | niacinamide nuclear factor erythroid-2-related factor 2 polydeoxyribonucleotide skin pigmentation skin pigmentation vitamin C |
| url | https://www.mdpi.com/1420-3049/27/4/1276 |
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