Performance and gut health status of broilers fed diets supplemented with two graded levels of a monoglyceride blend
ABSTRACT: The present study was undertaken to evaluate the efficacy of 2 graded levels (0.03 and 0.05% of diet) of a monoglyceride blend containing butyric, caprylic, and capric acids in broilers’ diet for optimizing gut structure and animal growth performance. For this purpose, a total of 210, one-...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-02-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122006538 |
| _version_ | 1811175510969942016 |
|---|---|
| author | Pinar Sacakli Özge Özgenç Çınar Ahmet Ceylan Muhammad Shazaib Ramay Josoa André Harijaona Alev Gurol Bayraktaroglu Yauheni Shastak Ali Calik |
| author_facet | Pinar Sacakli Özge Özgenç Çınar Ahmet Ceylan Muhammad Shazaib Ramay Josoa André Harijaona Alev Gurol Bayraktaroglu Yauheni Shastak Ali Calik |
| author_sort | Pinar Sacakli |
| collection | DOAJ |
| description | ABSTRACT: The present study was undertaken to evaluate the efficacy of 2 graded levels (0.03 and 0.05% of diet) of a monoglyceride blend containing butyric, caprylic, and capric acids in broilers’ diet for optimizing gut structure and animal growth performance. For this purpose, a total of 210, one-day-old male Ross 308 broiler chicks were randomly allocated to 3 experimental treatments using 7 replicates each and 10 birds/replicate. The treatment groups involved supplementation of blend of short and medium chain fatty acids at the level of 0, 0.03, and 0.05% of the diet for 42 d. The incorporation of mixes of monoglycerides into broilers’ diet linearly improved BWG between d 0 and 21 (P = 0.034). At the end of trial, however, no significant changes were observed in performance indexes (BWG, FI, FCR). Jejunal morphometric parameters (villus height, crypt depth, and their ratio) remained unaltered with the monoglyceride supplementation on d 21. The results further showed that monoglycerides supplementation increased the goblet cell counts along the jejunal villi (P = 0.034) and crypt regions (P = 0.022), as well as it effectively modulated the mRNA abundances of tight junction protein (ZO-1, P = 0.033) and nutrient transporters (SGLT, PePT1; P = 0.005, 0.023, respectively) in the jejunum. Moreover, the downregulation in mRNA abundance of TNFα (P = 0.030) was observed with the monoglyceride supplementation. The SCFAs analysis of cecal contents showed no notable differences with monoglyceride blend supplementation when compared to the unsupplemented group. Collectively, high goblet cell numbers in the jejunum along with downregulation of the mRNA abundances of pro-inflammatory cytokines, upregulation of tight junction proteins, and nutrient transporters showed favorable responses of low doses of monoglycerides blend in broiler feeding. Further studies should be conducted in different rearing conditions to examine the effectiveness of such low levels of a monoglyceride blend in the modulation of gut structure, its functionality and animal performance. |
| first_indexed | 2024-04-10T19:38:10Z |
| format | Article |
| id | doaj.art-e0681c55e2bf4336944941b25ad173e0 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-10T19:38:10Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-e0681c55e2bf4336944941b25ad173e02023-01-30T04:11:24ZengElsevierPoultry Science0032-57912023-02-011022102359Performance and gut health status of broilers fed diets supplemented with two graded levels of a monoglyceride blendPinar Sacakli0Özge Özgenç Çınar1Ahmet Ceylan2Muhammad Shazaib Ramay3Josoa André Harijaona4Alev Gurol Bayraktaroglu5Yauheni Shastak6Ali Calik7Department of Animal Nutrition & Nutritional Diseases, Faculty of Veterinary Medicine, Ankara University, Ankara, 06110, TurkeyDepartment of Histology Embryology, Faculty of Veterinary Medicine, Ankara University, Ankara, 06110, TurkeyDepartment of Histology Embryology, Faculty of Veterinary Medicine, Ankara University, Ankara, 06110, TurkeyDepartment of Animal Nutrition & Nutritional Diseases, Faculty of Veterinary Medicine, Ankara University, Ankara, 06110, TurkeyDepartment of Animal Nutrition & Nutritional Diseases, Faculty of Veterinary Medicine, Ankara University, Ankara, 06110, TurkeyDepartment of Histology Embryology, Faculty of Veterinary Medicine, Ankara University, Ankara, 06110, TurkeyBASF SE, 67056 Ludwigshafen, GermanyDepartment of Animal Nutrition & Nutritional Diseases, Faculty of Veterinary Medicine, Ankara University, Ankara, 06110, Turkey; Corresponding author:ABSTRACT: The present study was undertaken to evaluate the efficacy of 2 graded levels (0.03 and 0.05% of diet) of a monoglyceride blend containing butyric, caprylic, and capric acids in broilers’ diet for optimizing gut structure and animal growth performance. For this purpose, a total of 210, one-day-old male Ross 308 broiler chicks were randomly allocated to 3 experimental treatments using 7 replicates each and 10 birds/replicate. The treatment groups involved supplementation of blend of short and medium chain fatty acids at the level of 0, 0.03, and 0.05% of the diet for 42 d. The incorporation of mixes of monoglycerides into broilers’ diet linearly improved BWG between d 0 and 21 (P = 0.034). At the end of trial, however, no significant changes were observed in performance indexes (BWG, FI, FCR). Jejunal morphometric parameters (villus height, crypt depth, and their ratio) remained unaltered with the monoglyceride supplementation on d 21. The results further showed that monoglycerides supplementation increased the goblet cell counts along the jejunal villi (P = 0.034) and crypt regions (P = 0.022), as well as it effectively modulated the mRNA abundances of tight junction protein (ZO-1, P = 0.033) and nutrient transporters (SGLT, PePT1; P = 0.005, 0.023, respectively) in the jejunum. Moreover, the downregulation in mRNA abundance of TNFα (P = 0.030) was observed with the monoglyceride supplementation. The SCFAs analysis of cecal contents showed no notable differences with monoglyceride blend supplementation when compared to the unsupplemented group. Collectively, high goblet cell numbers in the jejunum along with downregulation of the mRNA abundances of pro-inflammatory cytokines, upregulation of tight junction proteins, and nutrient transporters showed favorable responses of low doses of monoglycerides blend in broiler feeding. Further studies should be conducted in different rearing conditions to examine the effectiveness of such low levels of a monoglyceride blend in the modulation of gut structure, its functionality and animal performance.http://www.sciencedirect.com/science/article/pii/S0032579122006538short- & medium chain fatty acidsintestinal histomorphologytight junction proteinscytokinesnutrient transporters |
| spellingShingle | Pinar Sacakli Özge Özgenç Çınar Ahmet Ceylan Muhammad Shazaib Ramay Josoa André Harijaona Alev Gurol Bayraktaroglu Yauheni Shastak Ali Calik Performance and gut health status of broilers fed diets supplemented with two graded levels of a monoglyceride blend Poultry Science short- & medium chain fatty acids intestinal histomorphology tight junction proteins cytokines nutrient transporters |
| title | Performance and gut health status of broilers fed diets supplemented with two graded levels of a monoglyceride blend |
| title_full | Performance and gut health status of broilers fed diets supplemented with two graded levels of a monoglyceride blend |
| title_fullStr | Performance and gut health status of broilers fed diets supplemented with two graded levels of a monoglyceride blend |
| title_full_unstemmed | Performance and gut health status of broilers fed diets supplemented with two graded levels of a monoglyceride blend |
| title_short | Performance and gut health status of broilers fed diets supplemented with two graded levels of a monoglyceride blend |
| title_sort | performance and gut health status of broilers fed diets supplemented with two graded levels of a monoglyceride blend |
| topic | short- & medium chain fatty acids intestinal histomorphology tight junction proteins cytokines nutrient transporters |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122006538 |
| work_keys_str_mv | AT pinarsacakli performanceandguthealthstatusofbroilersfeddietssupplementedwithtwogradedlevelsofamonoglycerideblend AT ozgeozgenccınar performanceandguthealthstatusofbroilersfeddietssupplementedwithtwogradedlevelsofamonoglycerideblend AT ahmetceylan performanceandguthealthstatusofbroilersfeddietssupplementedwithtwogradedlevelsofamonoglycerideblend AT muhammadshazaibramay performanceandguthealthstatusofbroilersfeddietssupplementedwithtwogradedlevelsofamonoglycerideblend AT josoaandreharijaona performanceandguthealthstatusofbroilersfeddietssupplementedwithtwogradedlevelsofamonoglycerideblend AT alevgurolbayraktaroglu performanceandguthealthstatusofbroilersfeddietssupplementedwithtwogradedlevelsofamonoglycerideblend AT yauhenishastak performanceandguthealthstatusofbroilersfeddietssupplementedwithtwogradedlevelsofamonoglycerideblend AT alicalik performanceandguthealthstatusofbroilersfeddietssupplementedwithtwogradedlevelsofamonoglycerideblend |