Enteroviral central nervous system infections in patients with Lyme neuroborreliosis
Patients with Lyme neuroborreliosis (LNB) are rarely tested for the presence of neurovirulent viruses other than tick-borne encephalitis virus (TBEV); however, such coinfections could be of clinical importance. The aim of the study was to search for the presence of neurotropic viruses in a LNB patie...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Ticks and Tick-Borne Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1877959X23001346 |
| _version_ | 1797676321963245568 |
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| author | Karol Perlejewski Marek Radkowski Agnieszka Pawełczyk Małgorzata Rydzanicz Tomasz Dzieciątkowski Michał Makowiecki Marcin Paciorek Renata Welc-Falęciak Andrzej Horban Tomasz Laskus |
| author_facet | Karol Perlejewski Marek Radkowski Agnieszka Pawełczyk Małgorzata Rydzanicz Tomasz Dzieciątkowski Michał Makowiecki Marcin Paciorek Renata Welc-Falęciak Andrzej Horban Tomasz Laskus |
| author_sort | Karol Perlejewski |
| collection | DOAJ |
| description | Patients with Lyme neuroborreliosis (LNB) are rarely tested for the presence of neurovirulent viruses other than tick-borne encephalitis virus (TBEV); however, such coinfections could be of clinical importance. The aim of the study was to search for the presence of neurotropic viruses in a LNB patients.Fourteen patients admitted with signs and symptoms of neuroinfection who were eventually diagnosed to have LNB (according to the guidelines of the European Federation of Neurological Societies) were subjects of the study. Sera and cerebrospinal fluid (CSF) collected at the time of initial presentation were tested for viral pathogens most common in our geographical area: human enteroviruses (EV), herpes simplex virus type 1 and 2, varicella-zoster virus, Epstein-Barr virus, cytomegalovirus, human herpesvirus type 6, human adenoviruses, and TBEV using PCR/RT-PCR and serological assays. RNA and DNA-based metagenomic next-generation sequencing (mNGS) was used to detect other viral pathogens.EV was detected in CSF from two (14 %) LNB patients and viral loads were similar (220 and 270 copies/ml). The mMGS analysis were performed on CSFs from 10 patients and generated a total 213,750,885 NGS reads, 0.05 % of which were viral. However, none of potential pathogens fulfilled the criteria for positive viral detection by mNGS.Using a number of PCR/RT-PCR assays and mNGS we identified EV infection in two out of 14 LNB patients. The possible co-occurrence of enterovirus and Lyme neuroborreliosis infections may warrant further research. |
| first_indexed | 2024-03-11T22:27:19Z |
| format | Article |
| id | doaj.art-e068cc597d524cdda775c39ea93e409f |
| institution | Directory Open Access Journal |
| issn | 1877-9603 |
| language | English |
| last_indexed | 2024-03-11T22:27:19Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ticks and Tick-Borne Diseases |
| spelling | doaj.art-e068cc597d524cdda775c39ea93e409f2023-09-24T05:14:41ZengElsevierTicks and Tick-Borne Diseases1877-96032023-11-01146102253Enteroviral central nervous system infections in patients with Lyme neuroborreliosisKarol Perlejewski0Marek Radkowski1Agnieszka Pawełczyk2Małgorzata Rydzanicz3Tomasz Dzieciątkowski4Michał Makowiecki5Marcin Paciorek6Renata Welc-Falęciak7Andrzej Horban8Tomasz Laskus9Department of Immunopathology of Infectious and Parasitic Diseases, Medical University of Warsaw, Pawińskiego 3c, Warsaw 02-106, Poland; Corresponding author.Department of Immunopathology of Infectious and Parasitic Diseases, Medical University of Warsaw, Pawińskiego 3c, Warsaw 02-106, PolandDepartment of Immunopathology of Infectious and Parasitic Diseases, Medical University of Warsaw, Pawińskiego 3c, Warsaw 02-106, PolandDepartment of the Medical Genetics, Medical University of Warsaw, Pawińskiego 3c, Warsaw 02-106, PolandDepartment of Microbiology, Medical University of Warsaw, Chalubińskiego 5, Warsaw 02-004, PolandDepartment of Adults Infectious Diseases, Medical University of Warsaw, Wolska 37, Warsaw 01-201, PolandDepartment of Adults Infectious Diseases, Medical University of Warsaw, Wolska 37, Warsaw 01-201, PolandDepartment of Parasitology, Faculty of Biology, University of Warsaw, Miecznikowa 1, Warsaw 02-096, PolandDepartment of Adults Infectious Diseases, Medical University of Warsaw, Wolska 37, Warsaw 01-201, PolandDepartment of Adults Infectious Diseases, Medical University of Warsaw, Wolska 37, Warsaw 01-201, PolandPatients with Lyme neuroborreliosis (LNB) are rarely tested for the presence of neurovirulent viruses other than tick-borne encephalitis virus (TBEV); however, such coinfections could be of clinical importance. The aim of the study was to search for the presence of neurotropic viruses in a LNB patients.Fourteen patients admitted with signs and symptoms of neuroinfection who were eventually diagnosed to have LNB (according to the guidelines of the European Federation of Neurological Societies) were subjects of the study. Sera and cerebrospinal fluid (CSF) collected at the time of initial presentation were tested for viral pathogens most common in our geographical area: human enteroviruses (EV), herpes simplex virus type 1 and 2, varicella-zoster virus, Epstein-Barr virus, cytomegalovirus, human herpesvirus type 6, human adenoviruses, and TBEV using PCR/RT-PCR and serological assays. RNA and DNA-based metagenomic next-generation sequencing (mNGS) was used to detect other viral pathogens.EV was detected in CSF from two (14 %) LNB patients and viral loads were similar (220 and 270 copies/ml). The mMGS analysis were performed on CSFs from 10 patients and generated a total 213,750,885 NGS reads, 0.05 % of which were viral. However, none of potential pathogens fulfilled the criteria for positive viral detection by mNGS.Using a number of PCR/RT-PCR assays and mNGS we identified EV infection in two out of 14 LNB patients. The possible co-occurrence of enterovirus and Lyme neuroborreliosis infections may warrant further research.http://www.sciencedirect.com/science/article/pii/S1877959X23001346Lyme neuroborreliosisBorreliaCoinfectionEnterovirus |
| spellingShingle | Karol Perlejewski Marek Radkowski Agnieszka Pawełczyk Małgorzata Rydzanicz Tomasz Dzieciątkowski Michał Makowiecki Marcin Paciorek Renata Welc-Falęciak Andrzej Horban Tomasz Laskus Enteroviral central nervous system infections in patients with Lyme neuroborreliosis Ticks and Tick-Borne Diseases Lyme neuroborreliosis Borrelia Coinfection Enterovirus |
| title | Enteroviral central nervous system infections in patients with Lyme neuroborreliosis |
| title_full | Enteroviral central nervous system infections in patients with Lyme neuroborreliosis |
| title_fullStr | Enteroviral central nervous system infections in patients with Lyme neuroborreliosis |
| title_full_unstemmed | Enteroviral central nervous system infections in patients with Lyme neuroborreliosis |
| title_short | Enteroviral central nervous system infections in patients with Lyme neuroborreliosis |
| title_sort | enteroviral central nervous system infections in patients with lyme neuroborreliosis |
| topic | Lyme neuroborreliosis Borrelia Coinfection Enterovirus |
| url | http://www.sciencedirect.com/science/article/pii/S1877959X23001346 |
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