Preparation and Application of Time-resolved Fluorescent Nanospheres Based Rapid Quantitative Detection Card of Fumonisin B1
In order to meet the needs of rapid quantitative detection of fumonisin B1 (FB1) in grains, this paper takes fumonisin B1 as the research object, and creates a rapid quantitative detection card of FB1 on the basis of time-resolved fluorescent nanospheres, with no need to adjust the PH of samples be...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academy of National Food and Strategic Reserves Administration
2022-07-01
|
| Series: | Liang you shipin ke-ji |
| Subjects: | |
| Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20220425?st=article_issue |
| _version_ | 1797869509621579776 |
|---|---|
| author | LIU Hong-mei ZHENG Meng-yao YUAN Hua-shan WANG Ping-dong ZHAO Wan XIAO Li-wen YE Jin WANG Song-xue |
| author_facet | LIU Hong-mei ZHENG Meng-yao YUAN Hua-shan WANG Ping-dong ZHAO Wan XIAO Li-wen YE Jin WANG Song-xue |
| author_sort | LIU Hong-mei |
| collection | DOAJ |
| description | In order to meet the needs of rapid quantitative detection of fumonisin B1 (FB1) in grains, this paper takes fumonisin B1 as the research object, and creates a rapid quantitative detection card of FB1 on the basis of time-resolved fluorescent nanospheres, with no need to adjust the PH of samples before detection. The detection performance of the detection card in grains (rice, corn, wheat) was analyzed and compared with enzyme-linked immunosorbent assay kit and high performance liquid chromatography. The LOD for different samples was in the range of 35.37~37.17 μg/kg, and the LOQ was 117.9~123.9 μg/kg, which meant good sensitivity; the linear range was 250~6 000 μg/kg, while the correlation coefficient R2 was in the range of 0.997 2~0.999 5; the spiking recoveries at 6 spiked levels in different samples were in the range of 83.38%~114.66%, with the coefficient of variation (CV) less than 15%, which meant good accuracy and repeatability. When the national standard liquid chromatography and FB1 fluorescence quantitative rapid detection card simultaneously detects different samples contaminated by FB1, the coincidence of the detection results was in the ranger of 92.53%~106.26%, with the CV less than 10.37%. The cross-reaction rate of other five common mycotoxins was less than 5%. Meanwhile, the difference between the spiked concentration and their average detected concentration was extremely significant, indicating good specificity. Hence, the FB1 fluorescence quantitative detection card prepared in this study could meet the needs of on-site rapid quantitative detection of fumonisin B1 in grains. |
| first_indexed | 2024-04-10T00:13:49Z |
| format | Article |
| id | doaj.art-e06a580006ac4be296873a0a265f4037 |
| institution | Directory Open Access Journal |
| issn | 1007-7561 |
| language | English |
| last_indexed | 2024-04-10T00:13:49Z |
| publishDate | 2022-07-01 |
| publisher | Academy of National Food and Strategic Reserves Administration |
| record_format | Article |
| series | Liang you shipin ke-ji |
| spelling | doaj.art-e06a580006ac4be296873a0a265f40372023-03-16T07:17:06ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612022-07-0130416417110.16210/j.cnki.1007-7561.2022.04.022Preparation and Application of Time-resolved Fluorescent Nanospheres Based Rapid Quantitative Detection Card of Fumonisin B1LIU Hong-mei0ZHENG Meng-yao1YUAN Hua-shan2WANG Ping-dong3ZHAO Wan4XIAO Li-wen5YE Jin6WANG Song-xue7Institute of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 102629, China;Institute of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 102629, China;Nanjing Storage Grain Oil Quality Supervision Center Co., Ltd., Nanjing, Jiangsu 211151, China;Nanjing Storage Grain Oil Quality Supervision Center Co., Ltd., Nanjing, Jiangsu 211151, China;Nanjing Microtest Biotechnology Co., Ltd., Nanjing, Jiangsu 210031, ChinaNanjing Microtest Biotechnology Co., Ltd., Nanjing, Jiangsu 210031, ChinaInstitute of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 102629, ChinaInstitute of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 102629, China In order to meet the needs of rapid quantitative detection of fumonisin B1 (FB1) in grains, this paper takes fumonisin B1 as the research object, and creates a rapid quantitative detection card of FB1 on the basis of time-resolved fluorescent nanospheres, with no need to adjust the PH of samples before detection. The detection performance of the detection card in grains (rice, corn, wheat) was analyzed and compared with enzyme-linked immunosorbent assay kit and high performance liquid chromatography. The LOD for different samples was in the range of 35.37~37.17 μg/kg, and the LOQ was 117.9~123.9 μg/kg, which meant good sensitivity; the linear range was 250~6 000 μg/kg, while the correlation coefficient R2 was in the range of 0.997 2~0.999 5; the spiking recoveries at 6 spiked levels in different samples were in the range of 83.38%~114.66%, with the coefficient of variation (CV) less than 15%, which meant good accuracy and repeatability. When the national standard liquid chromatography and FB1 fluorescence quantitative rapid detection card simultaneously detects different samples contaminated by FB1, the coincidence of the detection results was in the ranger of 92.53%~106.26%, with the CV less than 10.37%. The cross-reaction rate of other five common mycotoxins was less than 5%. Meanwhile, the difference between the spiked concentration and their average detected concentration was extremely significant, indicating good specificity. Hence, the FB1 fluorescence quantitative detection card prepared in this study could meet the needs of on-site rapid quantitative detection of fumonisin B1 in grains.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20220425?st=article_issuefumonisin b1rapid quantitative detectiontime-resolved fluorescentnanospheresfluorescence quantitative immunochromatography |
| spellingShingle | LIU Hong-mei ZHENG Meng-yao YUAN Hua-shan WANG Ping-dong ZHAO Wan XIAO Li-wen YE Jin WANG Song-xue Preparation and Application of Time-resolved Fluorescent Nanospheres Based Rapid Quantitative Detection Card of Fumonisin B1 Liang you shipin ke-ji fumonisin b1 rapid quantitative detection time-resolved fluorescent nanospheres fluorescence quantitative immunochromatography |
| title | Preparation and Application of Time-resolved Fluorescent Nanospheres Based Rapid Quantitative Detection Card of Fumonisin B1 |
| title_full | Preparation and Application of Time-resolved Fluorescent Nanospheres Based Rapid Quantitative Detection Card of Fumonisin B1 |
| title_fullStr | Preparation and Application of Time-resolved Fluorescent Nanospheres Based Rapid Quantitative Detection Card of Fumonisin B1 |
| title_full_unstemmed | Preparation and Application of Time-resolved Fluorescent Nanospheres Based Rapid Quantitative Detection Card of Fumonisin B1 |
| title_short | Preparation and Application of Time-resolved Fluorescent Nanospheres Based Rapid Quantitative Detection Card of Fumonisin B1 |
| title_sort | preparation and application of time resolved fluorescent nanospheres based rapid quantitative detection card of fumonisin b1 |
| topic | fumonisin b1 rapid quantitative detection time-resolved fluorescent nanospheres fluorescence quantitative immunochromatography |
| url | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20220425?st=article_issue |
| work_keys_str_mv | AT liuhongmei preparationandapplicationoftimeresolvedfluorescentnanospheresbasedrapidquantitativedetectioncardoffumonisinb1 AT zhengmengyao preparationandapplicationoftimeresolvedfluorescentnanospheresbasedrapidquantitativedetectioncardoffumonisinb1 AT yuanhuashan preparationandapplicationoftimeresolvedfluorescentnanospheresbasedrapidquantitativedetectioncardoffumonisinb1 AT wangpingdong preparationandapplicationoftimeresolvedfluorescentnanospheresbasedrapidquantitativedetectioncardoffumonisinb1 AT zhaowan preparationandapplicationoftimeresolvedfluorescentnanospheresbasedrapidquantitativedetectioncardoffumonisinb1 AT xiaoliwen preparationandapplicationoftimeresolvedfluorescentnanospheresbasedrapidquantitativedetectioncardoffumonisinb1 AT yejin preparationandapplicationoftimeresolvedfluorescentnanospheresbasedrapidquantitativedetectioncardoffumonisinb1 AT wangsongxue preparationandapplicationoftimeresolvedfluorescentnanospheresbasedrapidquantitativedetectioncardoffumonisinb1 |