Surface characterizations and cell growth of rough zirconia surfaces immobilized with different BMP-2 concentrations using natural crosslinker genipin
Zirconia (ZrO2) is widely used in the dental implant industry, due to its good mechanical properties and biocompatibility. However, the bioinert nature of ZrO2 may hinder osseointegration. This study investigated the responses of human bone marrow mesenchymal stem cells (hMSCs) to sandblasted ZrO2 s...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-11-01
|
| Series: | Materials & Design |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127522008383 |
| _version_ | 1829455702299508736 |
|---|---|
| author | Jing-Ying Chen Ying-Sui Sun Diem Thuy Nguyen Chia-Fei Liu Her-Hsiung Huang |
| author_facet | Jing-Ying Chen Ying-Sui Sun Diem Thuy Nguyen Chia-Fei Liu Her-Hsiung Huang |
| author_sort | Jing-Ying Chen |
| collection | DOAJ |
| description | Zirconia (ZrO2) is widely used in the dental implant industry, due to its good mechanical properties and biocompatibility. However, the bioinert nature of ZrO2 may hinder osseointegration. This study investigated the responses of human bone marrow mesenchymal stem cells (hMSCs) to sandblasted ZrO2 surfaces immobilized with bone morphogenetic protein-2 (BMP-2) using the natural crosslinker genipin. The immobilization of BMP on sandblasted ZrO2 surfaces increased the surface roughness but did not alter the wettability. The focal adhesion activation and later-stage mineralization of hMSCs were higher on BMP-2-modified ZrO2 surfaces than on untreated surfaces. The BMP-2-modified ZrO2 surfaces with low (100 ng/mL) and high (500 ng/mL) concentrations showed no significant difference in later-stage osteogenic response. However, ZrO2 surfaces with low BMP-2 concentration earlier stimulated the p38 (mitogen-activated protein kinase pathway) protein expression, osteogenic early marker osteopontin expression, and later-stage mineralization. This simple but effective process to immobilize BMP-2 on rough ZrO2 surfaces can benefit future dental implant applications. |
| first_indexed | 2024-04-11T08:33:46Z |
| format | Article |
| id | doaj.art-e06c4d0e1a7d446bbc06852b013bcf09 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-04-11T08:33:46Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-e06c4d0e1a7d446bbc06852b013bcf092022-12-22T04:34:23ZengElsevierMaterials & Design0264-12752022-11-01223111216Surface characterizations and cell growth of rough zirconia surfaces immobilized with different BMP-2 concentrations using natural crosslinker genipinJing-Ying Chen0Ying-Sui Sun1Diem Thuy Nguyen2Chia-Fei Liu3Her-Hsiung Huang4Department of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, Taiwan; School of Dental Technology, Taipei Medical University, Taipei 110, TaiwanDepartment of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, Taiwan; Institute of Oral Biology, National Yang Ming Chiao Tung University, Taipei 112, Taiwan; Department of Bioinformatics and Medical Engineering, Asia University, Taichung 413, Taiwan; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan; Department of Stomatology, Taipei Veterans General Hospital, Taipei 112, Taiwan; Department of Education and Research, Taipei City Hospital, Taipei 103, Taiwan; Corresponding author at: Department of Dentistry, National Yang Ming Chiao Tung University, No.155, Sec.2, Li-Nong Street, Taipei 112, Taiwan.Zirconia (ZrO2) is widely used in the dental implant industry, due to its good mechanical properties and biocompatibility. However, the bioinert nature of ZrO2 may hinder osseointegration. This study investigated the responses of human bone marrow mesenchymal stem cells (hMSCs) to sandblasted ZrO2 surfaces immobilized with bone morphogenetic protein-2 (BMP-2) using the natural crosslinker genipin. The immobilization of BMP on sandblasted ZrO2 surfaces increased the surface roughness but did not alter the wettability. The focal adhesion activation and later-stage mineralization of hMSCs were higher on BMP-2-modified ZrO2 surfaces than on untreated surfaces. The BMP-2-modified ZrO2 surfaces with low (100 ng/mL) and high (500 ng/mL) concentrations showed no significant difference in later-stage osteogenic response. However, ZrO2 surfaces with low BMP-2 concentration earlier stimulated the p38 (mitogen-activated protein kinase pathway) protein expression, osteogenic early marker osteopontin expression, and later-stage mineralization. This simple but effective process to immobilize BMP-2 on rough ZrO2 surfaces can benefit future dental implant applications.http://www.sciencedirect.com/science/article/pii/S0264127522008383Cell growthZirconiaBone morphogenetic protein-2GenipinNatural crosslinker |
| spellingShingle | Jing-Ying Chen Ying-Sui Sun Diem Thuy Nguyen Chia-Fei Liu Her-Hsiung Huang Surface characterizations and cell growth of rough zirconia surfaces immobilized with different BMP-2 concentrations using natural crosslinker genipin Materials & Design Cell growth Zirconia Bone morphogenetic protein-2 Genipin Natural crosslinker |
| title | Surface characterizations and cell growth of rough zirconia surfaces immobilized with different BMP-2 concentrations using natural crosslinker genipin |
| title_full | Surface characterizations and cell growth of rough zirconia surfaces immobilized with different BMP-2 concentrations using natural crosslinker genipin |
| title_fullStr | Surface characterizations and cell growth of rough zirconia surfaces immobilized with different BMP-2 concentrations using natural crosslinker genipin |
| title_full_unstemmed | Surface characterizations and cell growth of rough zirconia surfaces immobilized with different BMP-2 concentrations using natural crosslinker genipin |
| title_short | Surface characterizations and cell growth of rough zirconia surfaces immobilized with different BMP-2 concentrations using natural crosslinker genipin |
| title_sort | surface characterizations and cell growth of rough zirconia surfaces immobilized with different bmp 2 concentrations using natural crosslinker genipin |
| topic | Cell growth Zirconia Bone morphogenetic protein-2 Genipin Natural crosslinker |
| url | http://www.sciencedirect.com/science/article/pii/S0264127522008383 |
| work_keys_str_mv | AT jingyingchen surfacecharacterizationsandcellgrowthofroughzirconiasurfacesimmobilizedwithdifferentbmp2concentrationsusingnaturalcrosslinkergenipin AT yingsuisun surfacecharacterizationsandcellgrowthofroughzirconiasurfacesimmobilizedwithdifferentbmp2concentrationsusingnaturalcrosslinkergenipin AT diemthuynguyen surfacecharacterizationsandcellgrowthofroughzirconiasurfacesimmobilizedwithdifferentbmp2concentrationsusingnaturalcrosslinkergenipin AT chiafeiliu surfacecharacterizationsandcellgrowthofroughzirconiasurfacesimmobilizedwithdifferentbmp2concentrationsusingnaturalcrosslinkergenipin AT herhsiunghuang surfacecharacterizationsandcellgrowthofroughzirconiasurfacesimmobilizedwithdifferentbmp2concentrationsusingnaturalcrosslinkergenipin |