Enumeration of Viable Non-Culturable Vibrio cholerae Using Droplet Digital PCR Combined With Propidium Monoazide Treatment

Enumeration of Viable Non-Culturable Vibrio cholerae Using Droplet Digital PCR Combined With Propidium Monoazide Treatment

Many bacterial species, including Vibrio cholerae (the pathogen that causes cholera), enter a physiologically viable but non-culturable (VBNC) state at low temperature or in conditions of low nutrition; this is a survival strategy to resist environmental stress. Identification, detection, and differ...

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Bibliographic Details
Main Authors: Shuo Zhao, Jingyun Zhang, Zhe Li, Yu Han, Biao Kan
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-11-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
viable but non-cultivable state
ddPCR
qPCR
viable cell counting
propidium monoazide
Vibrio cholerae
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2021.753078/full

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