Microarray profiling of phage-display selections for rapid mapping of transcription factor-DNA interactions.
Modern computational methods are revealing putative transcription-factor (TF) binding sites at an extraordinary rate. However, the major challenge in studying transcriptional networks is to map these regulatory element predictions to the protein transcription factors that bind them. We have develope...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-04-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC2659770?pdf=render |
| _version_ | 1818838334422646784 |
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| author | Gordon Freckleton Soyeon I Lippman James R Broach Saeed Tavazoie |
| author_facet | Gordon Freckleton Soyeon I Lippman James R Broach Saeed Tavazoie |
| author_sort | Gordon Freckleton |
| collection | DOAJ |
| description | Modern computational methods are revealing putative transcription-factor (TF) binding sites at an extraordinary rate. However, the major challenge in studying transcriptional networks is to map these regulatory element predictions to the protein transcription factors that bind them. We have developed a microarray-based profiling of phage-display selection (MaPS) strategy that allows rapid and global survey of an organism's proteome for sequence-specific interactions with such putative DNA regulatory elements. Application to a variety of known yeast TF binding sites successfully identified the cognate TF from the background of a complex whole-proteome library. These factors contain DNA-binding domains from diverse families, including Myb, TEA, MADS box, and C2H2 zinc-finger. Using MaPS, we identified Dot6 as a trans-active partner of the long-predicted orphan yeast element Polymerase A & C (PAC). MaPS technology should enable rapid and proteome-scale study of bi-molecular interactions within transcriptional networks. |
| first_indexed | 2024-12-19T03:36:45Z |
| format | Article |
| id | doaj.art-e06dc62bd0cd45d2aa3506b0f9a80f30 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-19T03:36:45Z |
| publishDate | 2009-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-e06dc62bd0cd45d2aa3506b0f9a80f302022-12-21T20:37:21ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042009-04-0154e100044910.1371/journal.pgen.1000449Microarray profiling of phage-display selections for rapid mapping of transcription factor-DNA interactions.Gordon FreckletonSoyeon I LippmanJames R BroachSaeed TavazoieModern computational methods are revealing putative transcription-factor (TF) binding sites at an extraordinary rate. However, the major challenge in studying transcriptional networks is to map these regulatory element predictions to the protein transcription factors that bind them. We have developed a microarray-based profiling of phage-display selection (MaPS) strategy that allows rapid and global survey of an organism's proteome for sequence-specific interactions with such putative DNA regulatory elements. Application to a variety of known yeast TF binding sites successfully identified the cognate TF from the background of a complex whole-proteome library. These factors contain DNA-binding domains from diverse families, including Myb, TEA, MADS box, and C2H2 zinc-finger. Using MaPS, we identified Dot6 as a trans-active partner of the long-predicted orphan yeast element Polymerase A & C (PAC). MaPS technology should enable rapid and proteome-scale study of bi-molecular interactions within transcriptional networks.http://europepmc.org/articles/PMC2659770?pdf=render |
| spellingShingle | Gordon Freckleton Soyeon I Lippman James R Broach Saeed Tavazoie Microarray profiling of phage-display selections for rapid mapping of transcription factor-DNA interactions. PLoS Genetics |
| title | Microarray profiling of phage-display selections for rapid mapping of transcription factor-DNA interactions. |
| title_full | Microarray profiling of phage-display selections for rapid mapping of transcription factor-DNA interactions. |
| title_fullStr | Microarray profiling of phage-display selections for rapid mapping of transcription factor-DNA interactions. |
| title_full_unstemmed | Microarray profiling of phage-display selections for rapid mapping of transcription factor-DNA interactions. |
| title_short | Microarray profiling of phage-display selections for rapid mapping of transcription factor-DNA interactions. |
| title_sort | microarray profiling of phage display selections for rapid mapping of transcription factor dna interactions |
| url | http://europepmc.org/articles/PMC2659770?pdf=render |
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