SQSTM1/p62 interacts with FKBP38 and regulates cell cycle in Cashmere goat foetal fibroblasts
SQSTM1 (sequestosome 1, also known as p62) is a multifunctional scaffold protein implicated in diverse cell physiology processes, such as autophagy, cell signalling, and protein turnover. FKBP38 (FK506-binding protein 38) is a member of FKBPs family and plays a key role in various cellular processes...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Journal of Applied Animal Research |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09712119.2018.1495643 |
| _version_ | 1828324270205304832 |
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| author | Qiburi He Yanfeng Wang Keyu Zhao Uyanga Binderiya Lili Bao Pingping Zhao Dandan Yan Huifang Hao Xudong Guo Zhigang Wang |
| author_facet | Qiburi He Yanfeng Wang Keyu Zhao Uyanga Binderiya Lili Bao Pingping Zhao Dandan Yan Huifang Hao Xudong Guo Zhigang Wang |
| author_sort | Qiburi He |
| collection | DOAJ |
| description | SQSTM1 (sequestosome 1, also known as p62) is a multifunctional scaffold protein implicated in diverse cell physiology processes, such as autophagy, cell signalling, and protein turnover. FKBP38 (FK506-binding protein 38) is a member of FKBPs family and plays a key role in various cellular processes, including signalling transduction, embryonic development and apoptosis. In order to explore the role of SQSTM1/p62 gene in cell-cycle progression and proliferation of goat foetal fibroblast (GFbs), SQSTM1/p62 gene was cloned and characterized. Furthermore, the yeast two-hybrid screening system was used to identify the interaction between SQSTM1/p62 and FKBP38. The results suggested that SQSTM1/p62 directly interacts with FKBP38. Overexpression vector pIRES-EGFP-SQSTM1/p62 and shRNA eukaryotic expression vector pRNAT-U6.1-shSQSTM1/p62 which harboured siRNA targeting the SQSTM1/p62 mRNA were constructed. The overexpression of SQSTM1/p62 gene in GFbs significantly increase the S-phase cells compared with control cells (p < .05). Furthermore, SQSTM1/p62 gene silencing in GFbs leads to a significant decrease of S-phase cells and cell cycle arrest compared with control cells (p < .05). These data indicate that SQSTM1/p62 gene plays an important role in cell-cycle and proliferation of Cashmere GFbs. Abbreviations: Aba: aureobasidin A; EIF1: eukaryotic translation initiation factor 1; FKBP: FK506-binding protein; FKBP38: FK506-binding protein 38; GFbs: goat foetal fibroblast; mTOR: mammalian target of rapamycin; PDLIM7: PDZ and LIM domain 7; SD: standard dropout; SQSTM1/p62: sequestosome 1 |
| first_indexed | 2024-04-13T19:03:04Z |
| format | Article |
| id | doaj.art-e0710bb2decb413a86054b7d3c8b8e5d |
| institution | Directory Open Access Journal |
| issn | 0971-2119 0974-1844 |
| language | English |
| last_indexed | 2024-04-13T19:03:04Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Applied Animal Research |
| spelling | doaj.art-e0710bb2decb413a86054b7d3c8b8e5d2022-12-22T02:34:03ZengTaylor & Francis GroupJournal of Applied Animal Research0971-21190974-18442018-01-014611247125210.1080/09712119.2018.14956431495643SQSTM1/p62 interacts with FKBP38 and regulates cell cycle in Cashmere goat foetal fibroblastsQiburi He0Yanfeng Wang1Keyu Zhao2Uyanga Binderiya3Lili Bao4Pingping Zhao5Dandan Yan6Huifang Hao7Xudong Guo8Zhigang Wang9Inner Mongolia UniversityInner Mongolia UniversityInner Mongolia UniversityInner Mongolia UniversityInner Mongolia UniversityInner Mongolia UniversityInner Mongolia UniversityInner Mongolia UniversityInner Mongolia UniversityInner Mongolia UniversitySQSTM1 (sequestosome 1, also known as p62) is a multifunctional scaffold protein implicated in diverse cell physiology processes, such as autophagy, cell signalling, and protein turnover. FKBP38 (FK506-binding protein 38) is a member of FKBPs family and plays a key role in various cellular processes, including signalling transduction, embryonic development and apoptosis. In order to explore the role of SQSTM1/p62 gene in cell-cycle progression and proliferation of goat foetal fibroblast (GFbs), SQSTM1/p62 gene was cloned and characterized. Furthermore, the yeast two-hybrid screening system was used to identify the interaction between SQSTM1/p62 and FKBP38. The results suggested that SQSTM1/p62 directly interacts with FKBP38. Overexpression vector pIRES-EGFP-SQSTM1/p62 and shRNA eukaryotic expression vector pRNAT-U6.1-shSQSTM1/p62 which harboured siRNA targeting the SQSTM1/p62 mRNA were constructed. The overexpression of SQSTM1/p62 gene in GFbs significantly increase the S-phase cells compared with control cells (p < .05). Furthermore, SQSTM1/p62 gene silencing in GFbs leads to a significant decrease of S-phase cells and cell cycle arrest compared with control cells (p < .05). These data indicate that SQSTM1/p62 gene plays an important role in cell-cycle and proliferation of Cashmere GFbs. Abbreviations: Aba: aureobasidin A; EIF1: eukaryotic translation initiation factor 1; FKBP: FK506-binding protein; FKBP38: FK506-binding protein 38; GFbs: goat foetal fibroblast; mTOR: mammalian target of rapamycin; PDLIM7: PDZ and LIM domain 7; SD: standard dropout; SQSTM1/p62: sequestosome 1http://dx.doi.org/10.1080/09712119.2018.1495643Cell cycleSQSTM1/p62FKBP38goat foetal fibroblasts |
| spellingShingle | Qiburi He Yanfeng Wang Keyu Zhao Uyanga Binderiya Lili Bao Pingping Zhao Dandan Yan Huifang Hao Xudong Guo Zhigang Wang SQSTM1/p62 interacts with FKBP38 and regulates cell cycle in Cashmere goat foetal fibroblasts Journal of Applied Animal Research Cell cycle SQSTM1/p62 FKBP38 goat foetal fibroblasts |
| title | SQSTM1/p62 interacts with FKBP38 and regulates cell cycle in Cashmere goat foetal fibroblasts |
| title_full | SQSTM1/p62 interacts with FKBP38 and regulates cell cycle in Cashmere goat foetal fibroblasts |
| title_fullStr | SQSTM1/p62 interacts with FKBP38 and regulates cell cycle in Cashmere goat foetal fibroblasts |
| title_full_unstemmed | SQSTM1/p62 interacts with FKBP38 and regulates cell cycle in Cashmere goat foetal fibroblasts |
| title_short | SQSTM1/p62 interacts with FKBP38 and regulates cell cycle in Cashmere goat foetal fibroblasts |
| title_sort | sqstm1 p62 interacts with fkbp38 and regulates cell cycle in cashmere goat foetal fibroblasts |
| topic | Cell cycle SQSTM1/p62 FKBP38 goat foetal fibroblasts |
| url | http://dx.doi.org/10.1080/09712119.2018.1495643 |
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